Development of novel cancer immunotherapy targeting tumo vascular mimicry

Research Project

Project/Area Number	23501288
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Tumor immunology
Research Institution	Teikyo University
Principal Investigator	UTOGUCHI NAOKI 帝京大学, 薬学部, 教授 (80276633)
Project Period (FY)	2011 – 2013
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000) Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords	免疫療法 / ワクチン療法 / 擬似血管
Research Abstract	We established the isolation method of tumor vascular mimicry from solid tumor. As a result, after collagenase digestion, using the combination of MACS and Percoll density gradient, we succeeded to isolate of tumor vascular mimicry to a high purity. Further, by culturing the mouse melanoma on Matrigel, the tumor cells formed the tumor vascular mimicry in vitro. Tumor vascular mimicry cells were immunized mice were constructed of single-chain antibody phage display libraries incorporating the antibody gene. We are currently performing a selection of an antibody that specifically binds to the pseudo blood vessel.