Improved immunogenicity of fusions between ethanol-treated cancer cells and dendritic cells exposed to dual TLR stimulation
| Project/Area Number |
23501289
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Tumor immunology
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| Research Institution | Jikei University School of Medicine |
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Principal Investigator |
KOIDO Shigeo 東京慈恵会医科大学, 医学部, 准教授 (70266617)
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| Co-Investigator(Kenkyū-buntansha) |
HOMMA Sadamu 東京慈恵会医科大学, 医学部, 教授 (50192323)
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 樹状細胞 / がん細胞 / がん免疫 / 細胞障害性Ｔ細胞 / がんワクチン / 融合細胞 / WT1 / 細胞障害性T細胞 / 膵癌 / トール様受容体 / 高免疫原性 / 樹状細胞ワクチン / 癌細胞 / TGF-beta / 細胞融合 / 癌 / 免疫療法 / 細胞傷害性Ｔ細胞 / 抑制性Ｔ細胞 / 免疫原性 / ワクチン / エタノール |
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| Outline of Final Research Achievements |
Fusions of ethanol-treated tumor cells and dual Toll-like receptors (TLRs)-stimulated dendritic cells (DCs) (E-tumor/FCs) inhibited the production of multiple immunesuppressive soluble factors including TGF-β1 and up-regulated the production of IL-12p70 and HSP90α. Importantly, E-tumor/FCs activated T cells capable of producing high levels of IFN-γ, resulting in augmented MUC1-specific cytotoxic T lymphocyte (CTL) induction. Collectively, our results illustrate the synergy between ethanol-treated whole tumor cells and dual TLRs-stimulated DCs in inducing augmented CTL responses in vitro by fusion cell preparations. The alternative system is simple and may provide a platform for adoptive immunotherapy.
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Report
(5 results)
Research Products
(24 results)
