| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
An cationic lipid based adjuvant for inducing cytotoxic T cells (CTL) with protein antigens was prepared and developed to give strong CTL inducing activities by immunizing chicken Ovalbumin (OVA) protein as a model antigen. OVA proteins were expressed and purified in E.Coli and human mammalian cells, examined CTL inducing activities, and the strongest activity was observed with chicken derived antigen, followed by human cells and E.coli in this order.
When the protein antigens were expressed in human cell lines, the modification of glycan structures by addition of glycan synthesis inhibitor increased the CTL inducing activity of antigens with cationic adjuvants.
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