| Project/Area Number |
23501293
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Tumor diagnosis
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| Research Institution | Hokkaido University |
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Principal Investigator |
YOKOO Hideki 北海道大学, 大学病院, 助教 (70399947)
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| Co-Investigator(Kenkyū-buntansha) |
KAMIYAMA Toshiya 北海道大学, 大学院医学研究科, 准教授 (80322816)
NAKANISHI Kazuaki 北海道大学, 大学院医学研究科, 非常勤講師 (80374338)
KAKISAKA Tatsuhiko 北海道大学, 北海道大学病院, 医員 (40583092)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 肝細胞癌 / バイオマーカー / E-FABP / 発現解析 |
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| Research Abstract |
We analyzed the correlation between the expression of FABP5 and malignant behavior of HCC using HCC tissues and cell lines. A total of 243 HCC samples were subjected to immunohistochemistry, and protein expression of FABP5 in HCC cell lines was assessed by Western blot. The intensity of immunostaining in HCC tissues were divided into a strong staining group and a weak staining group. In the strong group, 5-year overall survival rate was 59.5%, compared with 89.9% in the weak group , and 5-year relapse free survival rate in the strong group was 23.7%, compared with 47.2% in the weak group . In vitro, Western blot showed protein expression of highly invasive cell lines was high, while lowly invasive cell was low. The knockdown of FABP5 in highly invasive cell lines also inhibited cell proliferation, migration and invasiveness significantly. FABP5 was closely related with malignancy, and also behaved as a significant prognostic and recurrence factor for HCC patients.
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