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Analysis of the new gene in connection with permeation of the lung adenocarcinoma in the genome PCR method and the FISH method

Research Project

Project/Area Number 23501294
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Tumor diagnosis
Research InstitutionUniversity of Tsukuba

Principal Investigator

MINAMI YUKO  筑波大学, 医学医療系, 准教授 (20466676)

Co-Investigator(Kenkyū-buntansha) NOGUCHI Masayuki  筑波大学, 医学医療系, 教授 (00198582)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords小型肺腺癌 / 3q26 / ECT2 / FISH / qPCR / 免疫染色 / 予後関連遺伝子 / Lung / Adenocarcinoma / adenocarcinoma in situ / early invasive / 早期肺腺癌 / Array-CGH解析 / 予後因子 / 免疫組織化学染色 / 定量的ゲノムPCR法 / CGH / 3q / 定量的リアルタイムPCR法 / 遺伝子増幅 / FISH法
Research Abstract

Genetic abnormality in early-stage lung adenocarcinoma was examined to search for new prognostic biomarkers. In situ lung adenocarcinomas and small but invasive adenocarcinomas were examined by array-comparative genomic hybridization (array-CGH), and candidate genes of interest were screened. To examine gene abnormalities, various types of lung carcinoma were examined by quantitative real-time genomic PCR (qPCR) and immunohistochemistry (IHC). Array-CGH demonstrated frequent amplification at chromosome 3q26, and we focused on the epithelial cell transforming sequence 2 (ECT2) oncogene. Overexpression of ECT2 in invasive adenocarcinoma was associated with disease-free survival and overall survival of patients with lung adenocarcinoma. These results were verified using another set of early-stage adenocarcinomas resected at another hospital. Abnormality of the ECT2 gene would be applicable as a new biomarker for prognostication of patients with lung adenocarcinoma.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (16 results)

All 2014 2013 2012 2011 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (13 results) Remarks (1 results)

  • [Journal Article] ECT2 amplification and overexpression as a new prognostic biomarker for early-stage lung adenocarcinoma2014

    • Author(s)
      Yoshihiko Murata, Yuko Minami, Reika Iwakawa, Jun Yokota, Shingo Usui, Koji Tsuta, Kouya Shiraishi, Shingo Sakashita, Kaishi Satomi, Tatsuo Iijima and Masayuki Noguchi
    • Journal Title

      Cancer Science

      Volume: 105 Issue: 4 Pages: 490-497

    • DOI

      10.1111/cas.12363

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] ECT2 serves as a new biomarker for early-stage lung adenocarcinoma. A FISH image for a tumor showing high ECT2 amplification. Red signal shows the ECT2 gene, and green signal shows chromosome 3 enumeration2014

    • Journal Title

      Cancer Sci

      Volume: 105(4) Issue: 4

    • DOI

      10.1111/cas.12384

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] ECT2 発現亢進は肺腺癌の新規予後予測マーカーである2013

    • Author(s)
      村田佳彦、南優子、岩川麗香、横田淳、野口雅之
    • Organizer
      第54回日本肺癌学会総会
    • Place of Presentation
      ホテルニューオータニ東京
    • Related Report
      2013 Final Research Report
  • [Presentation] ECT2 overexpression and gene alteration are associated with the outcome of early-stage lung adenocarcinoma2013

    • Author(s)
      Minami Y, Murata Y, Iwakawa R, Yokota J, Noguchi M
    • Organizer
      15th World Conference on Lung Cancer
    • Place of Presentation
      Australia
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] 肺癌における3q26 領域の遺伝子増幅の意義2013

    • Author(s)
      村田佳彦、南優子、野口雅之
    • Organizer
      第102回日本病理学会総会
    • Place of Presentation
      ホテルロイトン札幌
    • Related Report
      2013 Final Research Report
  • [Presentation] 肺癌における3q26領域の遺伝子増幅の意義2013

    • Author(s)
      村田佳彦、南優子、野口雅之
    • Organizer
      日本病理学会
    • Place of Presentation
      札幌
    • Related Report
      2013 Annual Research Report
  • [Presentation] ECT2発現亢進は肺腺癌の新規予後予測マーカーである2013

    • Author(s)
      村田佳彦、南優子、野口雅之
    • Organizer
      日本肺癌学会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] 肺癌における3q26 領域の遺伝子増幅2012

    • Author(s)
      村田佳彦、南優子、薄井真悟、野口雅之
    • Organizer
      第53回日本肺癌学会
    • Place of Presentation
      岡山コンベンションセンター
    • Related Report
      2013 Final Research Report
  • [Presentation] 肺癌における3q26 領域の遺伝子増幅(Genomic amplification at 3q26 region in lung carcinoma)2012

    • Author(s)
      村田佳彦、南優子、野口雅之
    • Organizer
      第71回日本癌学会総会
    • Place of Presentation
      ホテルロイトン札幌
    • Related Report
      2013 Final Research Report
  • [Presentation] 肺癌における3q26領域の遺伝子増幅2012

    • Author(s)
      村田佳彦、南優子、野口雅之
    • Organizer
      日本癌学会
    • Place of Presentation
      北海道札幌市
    • Related Report
      2012 Research-status Report
  • [Presentation] 肺がんにおける3q26領域の遺伝子増幅2012

    • Author(s)
      村田佳彦、南優子、薄井真悟、野口雅之
    • Organizer
      日本肺癌学会
    • Place of Presentation
      岡山県岡山市
    • Related Report
      2012 Research-status Report
  • [Presentation] 小型肺腺癌のArray CGH 解析2011

    • Author(s)
      南優子、村田佳彦、野口雅之
    • Organizer
      第52回日本肺癌学会総会
    • Place of Presentation
      大阪国際会議場
    • Related Report
      2013 Final Research Report
  • [Presentation] Array CGH analysis of small-sized adenocarcinoma of the lung2011

    • Author(s)
      Minami Y, Murata Y, Noguchi M
    • Organizer
      14^<th> World conference on lung
    • Place of Presentation
      Amsterdam, The Netherlands
    • Related Report
      2013 Final Research Report
  • [Presentation] Array CGH analysis od small-sized adenocarcinoma of the lung2011

    • Author(s)
      南優子
    • Organizer
      World Conference on Lung Cancer
    • Place of Presentation
      Amsterdam RAI, The Netherlands
    • Related Report
      2011 Research-status Report
  • [Presentation] 小型肺腺癌のArray CGH解析2011

    • Author(s)
      南優子
    • Organizer
      日本肺癌学会総会
    • Place of Presentation
      大阪国際会議場、大阪府
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.md.tsukuba.ac.jp/diagpatho/

    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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