Analysis of the new gene in connection with permeation of the lung adenocarcinoma in the genome PCR method and the FISH method
| Project/Area Number |
23501294
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Tumor diagnosis
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| Research Institution | University of Tsukuba |
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Principal Investigator |
MINAMI YUKO 筑波大学, 医学医療系, 准教授 (20466676)
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| Co-Investigator(Kenkyū-buntansha) |
NOGUCHI Masayuki 筑波大学, 医学医療系, 教授 (00198582)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 小型肺腺癌 / 3q26 / ECT2 / FISH / qPCR / 免疫染色 / 予後関連遺伝子 / Lung / Adenocarcinoma / adenocarcinoma in situ / early invasive / 早期肺腺癌 / Array-CGH解析 / 予後因子 / 免疫組織化学染色 / 定量的ゲノムPCR法 / CGH / 3q / 定量的リアルタイムPCR法 / 遺伝子増幅 / FISH法 |
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| Research Abstract |
Genetic abnormality in early-stage lung adenocarcinoma was examined to search for new prognostic biomarkers. In situ lung adenocarcinomas and small but invasive adenocarcinomas were examined by array-comparative genomic hybridization (array-CGH), and candidate genes of interest were screened. To examine gene abnormalities, various types of lung carcinoma were examined by quantitative real-time genomic PCR (qPCR) and immunohistochemistry (IHC). Array-CGH demonstrated frequent amplification at chromosome 3q26, and we focused on the epithelial cell transforming sequence 2 (ECT2) oncogene. Overexpression of ECT2 in invasive adenocarcinoma was associated with disease-free survival and overall survival of patients with lung adenocarcinoma. These results were verified using another set of early-stage adenocarcinomas resected at another hospital. Abnormality of the ECT2 gene would be applicable as a new biomarker for prognostication of patients with lung adenocarcinoma.
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Report
(4 results)
Research Products
(16 results)
