Development of new tumor markers based on microRNA expression in urine of the patients with urothelial carcinoma

• Project/Area Number: 23501298
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor diagnosis
• Research Institution: Kagoshima University
• Principal Investigator: enokida hideki 鹿児島大学, 医歯(薬)学総合研究科, 准教授 (80347103)
• Co-Investigator(Kenkyū-buntansha): 川上 一盛 鹿児島大学, 医学部・歯学部附属病院, 助教 (00404517)
• Research Collaborator: YOSHINO HIROFUMI 鹿児島大学, 医歯学総合研究科, 大学院生 ; HIDAKA HIDEO 鹿児島大学, 医歯学総合研究科, 大学院生 ; YAMASAKI TAKESHI 鹿児島大学, 医歯学総合研究科, 大学院生
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: microRNA / 膀胱癌 / 診断マーカー

Outline of Final Research Achievements

We developed novel urine markers for detecting urothelial carcinoma. Retrospective examination using 100 bladder cancer, 49 healthy volunteers, and 25 urinary tract infection patients revealed that miR-96 and miR-183 could distinguish cancer from benign disease with good sensitivity and specificity. These miRNAs expressions were well correlated with tumor stage and grade as recurrence free survival of non-muscle invasive blader cancer.
As for prospective examination of the out patients with macroscopic hematuria revealed that miR-X was a promising tumor markers with less false posive affected by macroscopic hematuria.

Research Products

• [Journal Article] Aberrant expression of microRNAs in bladder cancer (2013)
  • Author(s): Yoshino H, Seki N, Itesako T, Chiyomaru T, Nakagawa M, Enokida H, 他2名
  • Journal Title: Nat Rev Urol Volume: 10 Pages: 396-404
  • NAID: 120005353327
  • Peer Reviewed
• [Journal Article] Aberrant expression of microRNAs in bladder cancer. (2013)
  • Author(s): Yoshino H, Seki N, Itesako T, Chiyomaru T, Nakagawa M, Enokida H.
  • Journal Title: Nature Reviews Urology Volume: 104 Issue: 7 Pages: 1411-1419
  • DOI: 10.1038/nrurol.2013.113
  • NAID: 120005353327
  • Peer Reviewed
• [Journal Article] Novel molecular targets regulated by tumor suppressors microRNA-1 and microRNA-133a in bladder cancer (2012)
  • Author(s): Yamasaki T, Yoshino H, Enokida H, Hidaka H, 他7名
  • Journal Title: Int J Oncol Volume: 40 Pages: 1821-1830
  • Peer Reviewed
• [Journal Article] Functional role of LASP1 in cell viability and its regulation by microRNAs in bladder cancer (2012)
  • Author(s): Chiyomaru T, Enokida H, Kawakami K, 他6名.
  • Journal Title: Urol Oncol Volume: 30 Pages: 434-443
  • Peer Reviewed
• [Journal Article] Novel oncogenic function of mesoderm development candidate 1 and its regulation by MiR-574-3p in bladder cancer cell lines (2012)
  • Author(s): Tatarano S, Chiyomaru T, Kawakami K, Enokida H, Yoshino H, Hidaka H, Nohata N, Yamasaki, 他4名
  • Journal Title: Int J Oncol Volume: 40 Pages: 951-959
  • Peer Reviewed
• [Journal Article] Tumor suppressive microRNA-1 mediated novel apoptosis pathways through direct inhibition of splicing factor serine/arginine-rich 9 (SRSF9/SRp30c) in bladder cancer (2012)
  • Author(s): Yoshino H, Enokida H, Chiyomaru T, Tatarano S, Hidaka H, Yamasaki, 他6名
  • Journal Title: Biochem Biophys Res Commun Volume: 417 Pages: 588-593
  • Peer Reviewed
• [Journal Article] Novel oncogenic function of mesoderm development candidate 1 and its regulation by MiR-574-3p in bladder cancer cell lines. (2012)
  • Author(s): Tatarano S, Chiyomaru T, Kawakami K, Enokida H, Yoshino H, Hidaka H, Nohata N, Yamasaki T, Gotanda T, Tachiwada T, Seki N, Nakagawa M.
  • Journal Title: International Journal of Oncology Volume: 40 Issue: 4 Pages: 951-959
  • DOI: 10.3892/ijo.2011.1294
  • Peer Reviewed
• [Journal Article] MiR-96 and miR-183 detection in urine serve as potential tumor markers of urothelial carcinoma: correlation with stage and grade, and comparison with urinary cytology (2011)
  • Author(s): Yamada Y, Enokida H, Kojima S, Kawakami K, Chiyomaru T, Tatarano S, Yoshino H, 他4名
  • Journal Title: Cancer Sci Volume: 102 Pages: 522-529
  • NAID: 10029290902
  • Peer Reviewed
• [Journal Article] MiR-96 and miR-183 detection in urine serve as potential tumor markers of urothelial carcinoma: correlation with stage and grade, and comparison with urinary cytology. (2011)
  • Author(s): Yamada Y et al.
  • Journal Title: Cancer Science Volume: 102 Issue: 3 Pages: 522-529
  • DOI: 10.1111/j.1349-7006.2010.01816.x
  • Peer Reviewed
• [Presentation] microRNA expression signatures in bladder cancer : microRNA-145 function as a tumor suppressor through suppression of MAPK and ErbB signaling pathways (2013)
  • Author(s): Yoshino H, Enokida H
  • Organizer: American Urological Association (AUA) annual meeting
  • Place of Presentation: San Diego
  • Year and Date: 2013-05-21
• [Presentation] microRNAs detection in urine as diagnostic markers and predictors of recurrence of non-muscle invasive bladder cancer (2013)
  • Author(s): Enokida H
  • Organizer: American Urological Association (AUA) annual meeting
  • Place of Presentation: San Diego
  • Year and Date: 2013-05-21
• [Presentation] Molecular role of miR-195/497 cluster in human bladder cancer based on multiple dataset of microRNA expression signatures by deep sequencing (2013)
  • Author(s): Itesako T, Enokida H
  • Organizer: American Urological Association (AUA) annual meeting
  • Place of Presentation: San Diego
  • Year and Date: 2013-05-21
• [Presentation] A translational study of microRNAs detection in urine from outpatients with macroscopic hematuria: a new candidate microRNA from deep sequencing signature of urothelial carcinoma (2013)
  • Author(s): Enokida H
  • Organizer: 2013 American Urological Association Annual Meeting
  • Place of Presentation: San Diego, CA, USA
• [Presentation] Integrated analysis of two genome profiles (CGH and microRNA expression signature) reveled miR-218 and miR-574-3p as tumor suppressors in bladder cancer (2012)
  • Author(s): Tatarano S, Enokida H
  • Organizer: American Urological Association (AUA) annual meeting
  • Place of Presentation: Atlanta
  • Year and Date: 2012-05-21
• [Presentation] Tumor suppressive microRNA-1 directly regulates splicing factor arginine/serine-rich 9 (SRSF9/SRp30c) : a novel apoptotic pathway in bladder cancer (2012)
  • Author(s): Yoshino H, Enokida H
  • Organizer: American Urological Association (AUA) annual meeting
  • Place of Presentation: Atlanta
  • Year and Date: 2012-05-21
• [Presentation] microRNAs detection in urine as diagnostic markers and predictors of recurrence of non-muscle invasive bladder cancer (2012)
  • Author(s): Enokida H
  • Organizer: American Urological Association (AUA) annual meeting
  • Place of Presentation: Atlanta
  • Year and Date: 2012-05-21
• [Presentation] Molecular targets regulated by tumor suppressive microRNA-1 and microRNA-133a in bladder cancer (2012)
  • Author(s): Yamasaki T, Enokida H
  • Organizer: American Urological Association (AUA) annual meeting
  • Place of Presentation: Atlanta
  • Year and Date: 2012-05-21
• [Presentation] microRNAs detection in urine as diagnostic markers and predictors of recurrence of non-muscle invasive bladder cancer. (2012)
  • Author(s): Enokida H.
  • Organizer: 2012 American Urological Association Annual Meeting
  • Place of Presentation: Atlanta, GA, USA
• [Presentation] MicroRNAs (miR-96 and miR-183) detection in urine serves as tumor markers for diagnosis and prognosis in patients with urothelial carcinoma. (2012)
  • Author(s): Enokida H et al
  • Organizer: 2012 Annual meeting of European Association of Urology
  • Place of Presentation: Paris, France
• [Presentation] Functional role of LASP1 in bladder cnacer cell viability and its regulation by microRNAs (2011)
  • Author(s): Chiyomaru T, Enokida H
  • Organizer: American Urological Association (AUA) annual meeting
  • Place of Presentation: Washington DC
  • Year and Date: 2011-05-15
• [Presentation] MicroRNAs (miR-96 and miR-183) detection serves as urine markers in patients with urothelial carcinoma (2011)
  • Author(s): Enokida H
  • Organizer: American Urological Association (AUA) annual meeting
  • Place of Presentation: Washington DC
  • Year and Date: 2011-05-15
• [Presentation] The tumor suppressive miRNA cluster of miR-1 and miR-133a targets oncogenic TAGLN2 gene in bladder cancer (2011)
  • Author(s): Yoshino H, Enokida H
  • Organizer: American Urological Association (AUA) annual meeting
  • Place of Presentation: Washington DC
  • Year and Date: 2011-05-15
• [Remarks] 鹿児島大学大学院医歯学総合研究科腫瘍学講座泌尿器科学分野
  • URL: http://www.kufm.kagoshima-u.ac.jp/~urology/achievements_info.php
• [Remarks] 千葉大学大学院医学研究院先端応用医学講座 機能ゲノム学
  • URL: http://genomejet.jp/research/