Application of novel tumor associated carbohydrate antigen as tumor marker

• Project/Area Number: 23501303
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor diagnosis
• Research Institution: Research Institute, Osaka Medical Center for Cancer and Cardiovascular Disaeses
• Principal Investigator: MIYAMOTO Yasuhide 地方独立行政法人大阪府立病院機構大阪府立成人病センター(研究所), その他部局等, 研究員 (90322742)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 糖鎖 / 腫瘍マーカー / 癌

Research Abstract

We aimed to generate monoclonal antibodies specifically recognize tumor associated carbohydrate antigen, Sialyl Type 1H (ST1H), which are expressed in cancers of Lewis-negative individuals. Synthesized ST1H glycosphingolipid or colon cancer cells expressing ST1H were immunized to mice. The antibodies produced by the hybridoma were screened by enzyme linked immunosorbent assay (ELISA). Monoclonal antibodies that specifically recognize ST1H, but not isomers of ST1H such as ST2H, Sialyl Lewis A and Sialyl Lewis X, were obtained.

Research Products

• [Journal Article] Accumulation of free Neu5Ac-containing complex-type N-glycans in human pancreatic cancers (2013)
  • Author(s): Yabu, M., Korekane, H., Takahashi, H., Ohigashi, H., Ishikawa, O., Miyamoto, Y.
  • Journal Title: Glycoconj J Volume: 30(3) Issue: 3 Pages: 247-256
  • DOI: 10.1007/s10719-012-9435-9
  • Peer Reviewed
• [Journal Article] Occurrence of free deaminoneuraminic acid (KDN)-containing complex-type N-glycans in human prostate cancers (2013)
  • Author(s): Yabu, M., Korekane, H., Hatano, K., Kaneda, Y., Nonomura, N., Sato, C., Kitajima, K., Miyamoto, Y.
  • Journal Title: Glycobiology Volume: 23(6)(cws132 [pii]10.1093/glycob/cws132) Pages: 634-642
  • Peer Reviewed
• [Journal Article] 13-cis retinoic acid can enhance the anti-tumor activity of non-replicating Sendai virus particle against neuroblastoma (2013)
  • Author(s): Motonari Nomura, Takashi Shimbo, Yasuhide Miyamoto, Masahiro Fukuzawa, and Yasufumi Kaneda
  • Journal Title: Cancer Science Volume: 104 Issue: 2 Pages: 238-244
  • DOI: 10.1111/cas.12063
  • Peer Reviewed
• [Journal Article] Identification of Ectonucleotide Pyrophosphatase/Phosphodiesterase 3 (ENPP3) as a Regulator of N-Acetylglucosaminyltransferase GnT-IX (GnT-Vb) (2013)
  • Author(s): Korekane H, Park JY, Matsumoto A, Nakajima K, Takamatsu S, Ohtsubo K, Miyamoto Y, Hanashima S, Kanekiyo K, Kitazume S, Yamaguchi Y, Matsuo I, Taniguchi N
  • Journal Title: J. Biol. Chem. Volume: 288(39) Issue: 39 Pages: 27912-27926
  • DOI: 10.1074/jbc.m113.474304
  • Peer Reviewed
• [Journal Article] Occurrence of free deaminoneuraminic acid (KDN)-containing complex-type N-glycans in human prostate cancers. (2013)
  • Author(s): Yabu, M., Korekane, H., Hatano, K., Kaneda, Y., Nonomura, N., Sato, C., Kitajima, K., Miyamoto, Y
  • Journal Title: Glycobiology Volume: 23(6) Pages: 634-642
  • Peer Reviewed
• [Journal Article] Identification of Ectonucleotide Pyrophosphatase/Phosphodiesterase 3 (ENPP3) as a Regulator of N-Acetylglucosaminyltransferase GnT-IX (GnT-Vb). (2013)
  • Author(s): Korekane, H., Park, J.Y., Matsumoto, A., Nakajima, K., Takamatsu, S., Ohtsubo, K., Miyamoto, Y., Hanashima, S., Kanekiyo, K., Kitazume, S., Yamaguchi, Y., Matsuo, I., Taniguchi, N.
  • Journal Title: J Biol Chem Volume: 288(39) Pages: 27912-27926
  • Peer Reviewed
• [Journal Article] A strategy for large-scale phosphoproteomics and SRM-based validation of human breast cancer tissue samples. (2012)
  • Author(s): Narumi R, Murakami T, Kuga T, Adachi J, Shiromizu T, Muraoka S, Kume H, Kodera Y, Matsumoto M, Nakayama K, Miyamoto Y, Ishitobi M, Inaji H, Kato K, Tomonaga T.
  • Journal Title: J Proteome Res. Volume: 11 Issue: 11 Pages: 5311-5322
  • DOI: 10.1021/pr3005474
  • Peer Reviewed
• [Journal Article] Analysis of polarized secretion of fucosylated alpha-fetoprotein in HepG2 cells. (2012)
  • Author(s): Nakagawa T, Miyoshi E, et al.
  • Journal Title: J. Proteome Res Volume: 11(5) Issue: 5 Pages: 2798-806
  • DOI: 10.1021/pr201154k
  • Peer Reviewed
• [Journal Article] Strategy for SRM-based verification of biomarker candidates discovered by iTRAQ method in limited breast cancer tissue samples. (2012)
  • Author(s): Muraoka S, Kume H, Watanabe S, Adachi J, Kuwano M, Sato M, Kawasaki N, Kodera Y, Ishitobi M, Inaji H, Miyamoto Y, Kato K, Tomonaga T.
  • Journal Title: J Proteome Res. Volume: 11 Issue: 8 Pages: 4201-4210
  • DOI: 10.1021/pr300322q
  • Peer Reviewed
• [Journal Article] Androgen-regulated transcriptional control of sialyltransferases in prostate cancer cells (2012)
  • Author(s): Hatano, K., Miyamoto, Y., Mori, M., Nimura, K., Nakai, Y., Nonomura, N., Kaneda, Y.
  • Journal Title: PLoS One Volume: 7(2)
  • Peer Reviewed
• [Journal Article] Androgen-regulated transcriptional control of sialyltransferases in prostate cancer cells. (2012)
  • Author(s): Hatano, K., Miyamoto, Y., Mori, M., Nimura, K., Nakai, Y., Nonomura, N. Kaneda, Y.
  • Journal Title: PLoS One Volume: 7
  • Peer Reviewed
• [Journal Article] N-Glycosylation profiling of recombinant mouse extracellular superoxide dismutase produced in Chinese hamster ovary cells (2011)
  • Author(s): Korekane H
  • Journal Title: Glycoconj J Volume: 28 Issue: 3-4 Pages: 183-196
  • DOI: 10.1007/s10719-011-9333-6
  • Peer Reviewed
• [Journal Article] Expression of ganglisides, GD1a and sialyl paragloboside, is regulated by NF-κB-dependent transcriptional control of α2,3-sialyltransferase I, II and VI in human castration-resistant prostate cancer cells (2011)
  • Author(s): Hatano K, Miyamoto Y, Nonomura Nand Kaneda Y.
  • Journal Title: Int J Cancer Volume: 129 Issue: 8 Pages: 1838-1847
  • DOI: 10.1002/ijc.25860
  • Peer Reviewed
• [Presentation] 病態解明にむけたグライコミクス解析 (2013)
  • Author(s): 宮本泰豪
  • Organizer: 第32回日本糖質学会
  • Place of Presentation: 大阪市
  • Year and Date: 2013-08-06
• [Presentation] 病態解明に向けたグライコミクス-癌化に伴う糖鎖構造変化の高精度、高感度の解析を目指して (2013)
  • Author(s): 宮本 泰豪
  • Organizer: 第３２回日本糖質学会
  • Place of Presentation: 大阪市
• [Presentation] ヒト癌組織でのシアル酸付加された複合型のN 型遊離糖鎖の蓄積 (2012)
  • Author(s): 藪政彦・是金宏昭・佐藤ちひろ・北島健・宮本泰豪
  • Organizer: 第85回日本生化学大会
  • Place of Presentation: 福岡市
  • Year and Date: 2012-12-16
• [Presentation] 乳癌膜タンパク質の大規模プロテオーム解析とSRMを用いた検証法 (2011)
  • Author(s): 村岡賢・久米秀明・足立淳・宮本泰豪・加藤菊也・小寺義男・朝長毅
  • Organizer: 第７０回日本癌学会学術集会
  • Place of Presentation: 名古屋市
• [Presentation] ヒト癌組織でのシアル酸付加された複合型のN型遊離糖鎖の蓄積
  • Author(s): 藪政彦・是金宏昭・佐藤ちひろ・北島健・宮本泰豪
  • Organizer: 第８５回日本生化学大会
  • Place of Presentation: 福岡市