Development of the method to evaluate the marine ecosystem of mutagen derived from a microbe in the low temperature environment
| Project/Area Number |
23510025
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Environmental impact assessment/Environmental policy
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| Research Institution | Ibaraki University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 環境変異源 / 微生物 / 低温環境 / 酸化損傷塩基 / MutT / 大腸菌 / 枯草菌 / 乳酸菌 / 光合成細菌 / 低温細菌 / シアノバクテリア |
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| Research Abstract |
We analyzed the synthesis and accumulation of oxidative damage base in the cell in low temperature environment. After isolating deep sea bacteria each from the Boso ( 6,000m) and south mariana area ( 2,900m), and comparing resolution gene MutT of the oxidative damage base with the Escherichia coli origin gene, the specific activity of MutT showed a high value of approximately 2 times than Escherichia coli and the substrate specificity for the oxidation damage base was more remarkable than Escherichia coli and was high. Then, as a result of 8-OH-G quantity in microbe genomic DNA in the deep sea which collected sea water in MR09-04 (JAMSTEC) to check 8-OH-G production, there were more deep sea microbes approximately 10 times than an microbe from surface sea water.
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Report
(4 results)
Research Products
(26 results)
