Dynamics of TIP60 complex in the network of DNA damage response signaling
| Project/Area Number |
23510061
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Kyoto University |
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Principal Investigator |
IKURA TSUYOSHI 京都大学, 放射線生物研究センター, 准教授 (70335686)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | TIP60複合体 / ヒストンアセチル化 / ブロモドメイン蛋白質 / ヒストンH2AX / アセチル化 / TIP60ヒストンアセチル化酵素複合体 / DNA損傷応答 |
|---|
| Research Abstract |
We and other group have shown that TIP60 histone acetyltransferase complex regulates hyperacetylation of histone H2AX and H4 at DNA damage sites, which is crucial for the higherorder chromatin alteration. However, it remains unclear how assembly of TIP60 is regulated at DNA damage sites. We have previosuly identified the bromodomain protein, BRDX, in the purified TIP60 complex. We found that the depletion of BRDX in cells impairs the assembly of TIP60 at DNA damage sites. This finding suggest that BRDX is required for the assembly of TIP60 at DNA damage sites. Moreover, we showed that the acetylation of histone H2AX by TIP60 is required for the retention of NBS1 at DNA damage sites. Since it has been reported that TIP60 functions as anti-cancer signaling molecule, we are now investigating whether chromatin reorganization via TIP60-BRDX complex is involved in the anti-cancer signaling pathway or not.
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Report
(4 results)
Research Products
(72 results)
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[Journal Article] Activation of the SUMO modification system is required for the accumulation of RAD51 at sites containing DNA damage2013
Author(s)
Shima H, Suzuki H, Sun J, Kono K, Shi L, Kinomura A, Horikoshi Y, Ikura T, Ikura M, Kanaar R, Igarashi K, Saitoh H, Kurumizaka H, Tashiro S
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Journal Title
J Cell Sci
Volume: (Epub ahead of print)
Related Report
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[Journal Article] Involvement of homologous recombination in the synergism between cisplatin and poly(ADP-ribose) polymerase inhibition2013
Author(s)
Sakogawa K, Aoki Y, Misumi K, Hamai Y, Emi M, Hihara J, Shi L, Kono K, Horikoshi Y, Sun J, Ikura T, Okada M, Tashiro S
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Journal Title
Cancer Sci. 2013 Sep 5
Volume: 104
Issue: 12
Pages: 1593-1609
DOI
Related Report
Peer Reviewed
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[Journal Article] A novel interplay between the Fanconi anemia core complex and ATR-ATRIP kinase during DNA cross-link repair2013
Author(s)
Tomida J, Itaya A, Shigechi T, Unno J, Uchida E, Ikura M, Masuda Y, Matsuda S, Adachi J, Kobayashi M, Meetei AR, Maehara Y, Yamamoto KI, Kamiya K, Matsuura A, Matsuda T, Ikura T, Ishiai M, Takata M
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Journal Title
Nucleic Acids Res
Volume: (Epub ahead of print)
NAID
Related Report
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[Journal Article] Involvement of ribonucleotide reductase-M1 in 5-fluorouracil-induced DNA damage in esophageal cancer cell lines2013
Author(s)
Aoki, Y., Sakogawa, K., Hihara, J., Emi, M., Hamai,Y., Kono, K., Shi, L., Sun, J., Kitao, H., Ikura, T., Niida, H., Nakanishi, M., Okada, M., Tashiro, S
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Journal Title
Int J Oncol
Volume: 42
Pages: 1951-1960
Related Report
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[Journal Article] Structural basis of a nucleosome containing histone H2A. B/H2A. Bbd that transiently associates with reorganized chromatin.2013
Author(s)
Arimura Y., Kimura H., Oda T., Sato K., Osakabe A., Tachiwana H., Sato Y., Kinugasa Y., Ikura T., Sugiyama M., Sato M., Kurumizaka H.
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Journal Title
Sci Rep.
Volume: 3
Issue: 1
Pages: 3510-3510
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Activation of the SUMO modification system is required for the accumulation of RAD51 at sites containing DNA damage2013
Author(s)
Shima H, Suzuki H, Sun J, Kono K, Shi L, Kinomura A, Horikoshi Y, Ikura T, Ikura M, Kanaar R, Igarashi K, Saitoh H, Kurumizaka H, Tashiro S
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Journal Title
J Cell Sci
Volume: 126
Pages: 5284-5292
DOI
Related Report
Peer Reviewed
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[Journal Article] A novel interplay between the Fanconi anemia core complex and ATR-ATRIP kinase during DNA cross-link repair2013
Author(s)
J Tomida, A Itaya, T Shigechi, J Unno, E Uchida, M Ikura, Y Masuda, S Matsuda J Adachi, M Kobayashi, AR Meetei, Y Maehara, K Yamamoto K Kamiya, A Matsuura, T Matsuda. T Ikura, M Ishiai, M Takata
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Journal Title
Nucleic Acids Research
Volume: VOL. 41
Issue: 14
Pages: 6930-6941
DOI
Related Report
Peer Reviewed
-
[Journal Article] Involvement of ribonucleotide reductase-M1 in 5-fluorouracil induced DNA damage in esophageal cancer cell lines.2013
Author(s)
Aoki Y, Sakogawa K, Hihara J, Emi M, Hamai Y, Kono K, Shi L, Sun J, Kitao H, Ikura T, Niida H, Nakanishi M, Okada M, Tashiro S.
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Journal Title
Int J Oncol.
Volume: 42(6)
Issue: 6
Pages: 1951-60
DOI
Related Report
Peer Reviewed
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[Journal Article] Heterocomplex Formation by Arp4 and ƒÀ-Actin Involved in Integrity of the Brg1 Chromatin Remodeling Complex2012
Author(s)
Nishimoto, N., Watanabe, M., Watanabe, S., Sugimoto, N., Yugawa,T., Ikura, T., Koiwai, O., Kiyono,T and Fujita, M
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Journal Title
J. Cell Sci
Volume: 125
Pages: 3870-3882
Related Report
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[Journal Article] A new system for analyzing ionizing radiation-induced chromosome abnormalities2012
Author(s)
Shi L., Fujioka K., Sun J., Kinomura A., Inaba T., Ikura T., Ohtaki M., Yoshida M., Kodama Y., Livingston G.K., Kamiya K., Tashiro S
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Journal Title
Radiat. Res
Volume: 117
Pages: 533-538
Related Report
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[Journal Article] Purification of the human SMN-GEMIN2 complex and assessment of its stimulation of RAD51-mediated DNA recombination reactions2011
Author(s)
Takaku, M., Tsujita, T., Horikoshi, N., Takizawa, Y., Qing, Y., Hirota, K., Ikura, M., Ikura, T., Takeda, S., Kurumizaka, H
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Journal Title
Biochemistry
Volume: 50
Pages: 6797-6805
Related Report
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[Journal Article] Methionine Adenosyltransferase II Serves As a Transcriptional Corepressor of Maf Oncoprotein2011
Author(s)
Katoh, Y, Ikura, T., Hoshikawa,Y., Tashiro, S., Ohta, M., Kera, Y., Noda, T., and Igarashi, K
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Journal Title
Mol Cell
Volume: 41
Pages: 554-566
Related Report
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