Research Project
Grant-in-Aid for Scientific Research (C)
We and other group have shown that TIP60 histone acetyltransferase complex regulates hyperacetylation of histone H2AX and H4 at DNA damage sites, which is crucial for the higherorder chromatin alteration. However, it remains unclear how assembly of TIP60 is regulated at DNA damage sites. We have previosuly identified the bromodomain protein, BRDX, in the purified TIP60 complex. We found that the depletion of BRDX in cells impairs the assembly of TIP60 at DNA damage sites. This finding suggest that BRDX is required for the assembly of TIP60 at DNA damage sites. Moreover, we showed that the acetylation of histone H2AX by TIP60 is required for the retention of NBS1 at DNA damage sites. Since it has been reported that TIP60 functions as anti-cancer signaling molecule, we are now investigating whether chromatin reorganization via TIP60-BRDX complex is involved in the anti-cancer signaling pathway or not.
All 2014 2013 2012 2011 Other
All Journal Article (16 results) (of which Peer Reviewed: 8 results, Open Access: 2 results, Acknowledgement Compliant: 1 results) Presentation (53 results) (of which Invited: 11 results) Book (2 results) Remarks (1 results)
J Cell Sci
Volume: 127 Pages: 763-772
10.1242/jcs.135855
Volume: (Epub ahead of print)
Cancer Sci. 2013 Sep 5
Volume: 104 Issue: 12 Pages: 1593-1609
10.1111/cas.12281
Nucleic Acids Res
120005323036
Int J Oncol
Volume: 42 Pages: 1951-1960
Sci Rep.
Volume: 3 Issue: 1 Pages: 3510-3510
10.1038/srep03510
Volume: 126 Pages: 5284-5292
10.1242/jcs.133744
Nucleic Acids Research
Volume: VOL. 41 Issue: 14 Pages: 6930-6941
10.1093/nar/gkt467
Int J Oncol.
Volume: 42(6) Issue: 6 Pages: 1951-60
10.3892/ijo.2013.1899
J. Cell Sci
Volume: 125 Pages: 3870-3882
Cancer Sci
Volume: 103 Pages: 897-903
Radiat. Res
Volume: 117 Pages: 533-538
J. Cell Sci.
10.1242/jcs.104349
Cancer Science
Volume: 10 Issue: 5 Pages: 2237-2244
10.1111/j.1349-7006.2012.02244.x
Biochemistry
Volume: 50 Pages: 6797-6805
Mol Cell
Volume: 41 Pages: 554-566
http://house.rbc.kyoto-u.ac.jp/mutagenesis2/