| Project/Area Number |
23510063
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Okayama University |
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Principal Investigator |
NEGISHI Tomoe 岡山大学, 医歯(薬)学総合研究科, 准教授 (80116491)
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| Co-Investigator(Kenkyū-buntansha) |
IBUKI Yuko 静岡県立大学, 食品栄養科学部, 教授 (30236781)
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| Research Collaborator |
FANG Xing
TOYOOKA Tatsushi
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 近紫外光 / 紫外線 / DNA傷害 / 活性酸素種 / ヒストンリン酸化 / ショウジョウバエ / DNA傷害修復 / UVA / 体細胞突然変異 / DNA二本鎖切断 / 酸化傷害 / 尿酸 / ピリミジン二量体 / 長波長紫外線 / 突然変異 / DNA損傷 / 染色体組換え / 細胞毒性 / シグナル伝達 |
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| Outline of Final Research Achievements |
We have investigated the involvement of UVA in mutations and DNA damage in somatic cells using Drosophila melanogaster larvae. 365-nm-LED (a high-dose-rate UVA source) was mutagenic in mei-41 than in mei-9. The mei-41 gene was shown to be an orthologue of the human ATR gene, which is involved in the repair of DSBs through phosphorylation of histone H2Ax. LED-UVA was mutagenic in the urate-null strain at doses that were non-mutagenic in the urate-positive strain. We also observed that reactive oxygen species were produced in larvae by LED-UVA irradiation. At higher doses of LED-UVA, levels of phosphorylated H2AvD (γ-H2AvD) increased significantly in the urate-null strain but not in wild-type. It was reported that ATR functions on DSB repair in D. melanogaster. Taken together, we propose a possible pathway for UVA-induced mutation, whereby DNA double-strand breaks resulting from oxidative stress might be responsible for UVA-induced mutation in somatic cells of D. melanogaster larvae.
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