A Study of an Advanced Membrane Model for Cell-Mimetic Microchemical Systems

• Project/Area Number: 23510143
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Microdevices/Nanodevices
• Research Institution: Shinshu University
• Principal Investigator: OKUMURA Yukihisa 信州大学, 工学部, 教授 (40243042)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2013: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
• Keywords: 巨大脂質膜ベシクル / 巨大リポソーム / マイクロ化学システム / 人工細胞膜モデル / 巨大ベシクル / マイクロマニピュレーション / エレクトロフォーメーション / 内部膜コンパートメント / リポソーム

Research Abstract

A use of an oligovesicular vesicle as a novel advanced model of artificial cell membrane was examined. The reliability of the procedure to construct the model was improved. Formation of multiple inner vesicles that were to be the membrane foundation of inner chemical subsystems was possible.

Research Products

• [Journal Article] Electroformation of Giant Vesicles and Transformation to Oligovesicular Vesicles (2013)
  • Author(s): Yukihisa Okumura
  • Journal Title: Advances in Planar Lipid Bilayers and Liposomes Volume: 18 Pages: 63-79
  • DOI: 10.1016/b978-0-12-411515-6.00003-5
• [Journal Article] Immobilization of Giant Vesicles onto Hydrophobized Polymer Gel Particles (2011)
  • Author(s): Yukihisa Okumura, Satoshi Yabushita, Aya Watanabe
  • Journal Title: Bull. Chem. Soc. Jpn Volume: 84 Issue: 10 Pages: 1144-1146
  • DOI: 10.1246/bcsj.20110083
  • NAID: 130004152809
• [Journal Article] Electroformation of Giant Vesicles and Electrode Polarity (2011)
  • Author(s): Yukihisa Okumura, Yuuichi Iwata
  • Journal Title: Bull. Chem. Soc. Jpn Volume: 84 Issue: 10 Pages: 1147-1149
  • DOI: 10.1246/bcsj.20110097
  • NAID: 130004152814
• [Journal Article] Effect of Counter Electrode in Electroformation of Giant Vesicles (2011)
  • Author(s): Yukihisa Okumura, Shuuhei Oana
  • Journal Title: Membranes Volume: 1 Issue: 4 Pages: 345-353
  • DOI: 10.3390/membranes1040345
  • NAID: 120007101108
• [Journal Article] Rapid Electroformation of Giant Vesicles (2011)
  • Author(s): Yukihisa Okumura, Koji Urita
  • Journal Title: Chem. Lett Volume: 40 Issue: 5 Pages: 530-532
  • DOI: 10.1246/cl.2011.530
  • NAID: 130004426220
• [Journal Article] Formation of Oligovesicular Vesicles by Micromanipulation (2011)
  • Author(s): Yukihisa Okumura, Tohru Ohmiya, Toshiki Yamazaki
  • Journal Title: Membranes Volume: 1 Issue: 4 Pages: 265-274
  • DOI: 10.3390/membranes1040265
  • NAID: 120007101109
• [Journal Article] Electroformation of Giant Vesicles on Indium Tin Oxide (ITO)-Coated Poly(ethylene terephthalate) (PET) Electrodes (2011)
  • Author(s): Yukihisa Okumura, Yuuichi Iwata
  • Journal Title: Membranes Volume: 1 Issue: 2 Pages: 109-118
  • DOI: 10.3390/membranes1020109
  • NAID: 120007101110
• [Journal Article] Electroformation of Giant Vesicles on a Polymer Mesh (2011)
  • Author(s): Yukihisa Okumura, Takuya Sugiyama
  • Journal Title: Membranes Volume: 1 Issue: 3 Pages: 184-194
  • DOI: 10.3390/membranes1030184
  • NAID: 120007101094
• [Presentation] 巨大ベシクルのエレクトロフォーメーションと印加電圧の効果 (2014)
  • Author(s): 奥村幸久
  • Organizer: 日本化学会第94春季年会
  • Place of Presentation: 名古屋
  • Year and Date: 2014-03-29
• [Presentation] 巨大ベシクルのエレクトロフォーメーションと印加電圧の効果 (2014)
  • Author(s): 奥村幸久
  • Organizer: 日本化学会第94春季年会
  • Place of Presentation: 名古屋大学
• [Presentation] 巨大ベシクルの迅速なエレクトロフォーメーション (2013)
  • Author(s): 赤津智広・奥村幸久
  • Organizer: 日本化学会第93春季年会
  • Place of Presentation: 草津
  • Year and Date: 2013-03-23
• [Presentation] 巨大ベシクルからオリゴベシキュラーベシクルの形成 (2013)
  • Author(s): 堀内駿・奥村幸久
  • Organizer: 日本化学会第93春季年会
  • Place of Presentation: 草津
  • Year and Date: 2013-03-23
• [Presentation] 巨大ベシクルのマイクロマニピュレーションと膜の性状 (2012)
  • Author(s): 山崎稔季・奥村幸久
  • Organizer: 日本化学会第92春季年会
  • Place of Presentation: 横浜
  • Year and Date: 2012-03-27