Development of a potable ELISA machine using novel antigen-bound polymer
Project/Area Number |
23510146
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Microdevices/Nanodevices
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Research Institution | University of Hyogo |
Principal Investigator |
TAKEO MASAHIRO 兵庫県立大学, 工学(系)研究科(研究院), 准教授 (40236443)
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Co-Investigator(Kenkyū-buntansha) |
UTSUMI Yuichi 兵庫県立大学, 高度産業科学技術研究所, 教授 (80326298)
YUSA Shinichi 兵庫県立大学, 大学院工学研究科, 准教授 (00301432)
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Co-Investigator(Renkei-kenkyūsha) |
NEGORO Seiji 兵庫県立大学, 大学院工学研究科, 教授 (90156159)
KATO Dai-ichiro 兵庫県立大学, 大学院工学研究科, 助教 (60423901)
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Project Period (FY) |
2011 – 2013
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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Keywords | ELISA / nonylphenol / 分析機 / 内分泌撹乱物質 / マイクロリアクター / ポータブル分析機 / 環境分析 / 食品分析 / ノニルフェノール / ニトロフェノール / 抗原固定化 |
Research Abstract |
We developed a portable ELISA machine for the measurement of an endocrine disruptor, nonylphenol. A microfilter, which was made from PMMA-based polymer with nonylphenyl group as a side chain, was set in a vertically-stacked microreactor. The microreactor was set in a newly-developed automatic fluidic and detection system in a 25cm-cubic case and competitive ELISA was performed using this small system. As a result, we could construct a good standard curve with negative correlation in a concentration range of 0.01-1.00 microgram/l and the average coefficient of variation of the data obtained was 4.2%. These results were comparable to the sensitivity and repeatability of commercial sensitive analytical machines.
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Report
(4 results)
Research Products
(8 results)
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[Presentation] Development of automatic ELISA analytical system for a trace amount of nonylphenol7using a novel antigen-bound microfilter2013
Author(s)
M. Takeo, I. Kawaji, A. Nakasuji, T. Tone, Y. Ukita, D. Kato, S. Negoro, S. Yusa, M. Katayama, and Y. Utsumi
Organizer
Proc. of 10^<th> International Workshop on High Aspect Ratio Micro and Nano Structure Technology (HARMNST2013)
Place of Presentation
Berlin, Germany
Related Report
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[Presentation] Development of Automatic ELISA Analytical System for a Trace Amount of Nonylphenol Using a Novel Antigen-Bound Microfilter2013
Author(s)
Masahiro Takeo, Isao Kawaji, Akihiro Nakasuji, Takaaki Tone, Yoshiaki Ukita, Dai-ichiro Kato, Seiji Negoro, Shinichi Yusa, Makoto Katayama, Yuichi Utsumi
Organizer
10th International Workshop on High Aspect Ratio Micro and Nano System Technology (HARMNST2013)
Place of Presentation
Berlin, Germany
Related Report
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[Presentation] Automatic ELISA analytical system for a trace amount of environmental chemicals using a 3-dimensional microreactor with a novel antigen-bound microfilter2012
Author(s)
M. Takeo, I. Kawaji, A. Nakasuji, T. Tone, Y.Ukita, D. Kato, S. Negoro, S. Yusa, M. Katayama, and Y.Utsumi
Organizer
Proc. of the 16^<th> International Conference on Miniaturized Systems for Chemistry and Life Science (μTAS2012)
Place of Presentation
Okinawa, Japan
Related Report
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[Presentation] Automatic ELISA analytical system for a trace amount of environmental chemicals using a 3-dimensional microreactor with a novel antigen-bound microfilter2012
Author(s)
Masahiro Takeo, Isao Kawaji, Akihiro Nakasuji, Takaaki Tone, Yoshiaki Ukita, Dai-ichiro Kato, Seiji Negoro, Shinichi Yusa, Makoto Katayama, and Yuichi Utsumi
Organizer
Proceeding of the 16th International Conference on Miniaturized Systems for Chemistry and Life Science, T.4.114.pdf in USB
Place of Presentation
Okinawa Convention Center, Okinawa, Japan
Related Report
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