| Project/Area Number |
23510146
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Microdevices/Nanodevices
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| Research Institution | University of Hyogo |
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Principal Investigator |
TAKEO MASAHIRO 兵庫県立大学, 工学(系)研究科(研究院), 准教授 (40236443)
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| Co-Investigator(Kenkyū-buntansha) |
UTSUMI Yuichi 兵庫県立大学, 高度産業科学技術研究所, 教授 (80326298)
YUSA Shinichi 兵庫県立大学, 大学院工学研究科, 准教授 (00301432)
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| Co-Investigator(Renkei-kenkyūsha) |
NEGORO Seiji 兵庫県立大学, 大学院工学研究科, 教授 (90156159)
KATO Dai-ichiro 兵庫県立大学, 大学院工学研究科, 助教 (60423901)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | ELISA / nonylphenol / 分析機 / 内分泌撹乱物質 / マイクロリアクター / ポータブル分析機 / 環境分析 / 食品分析 / ノニルフェノール / ニトロフェノール / 抗原固定化 |
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| Research Abstract |
We developed a portable ELISA machine for the measurement of an endocrine disruptor, nonylphenol. A microfilter, which was made from PMMA-based polymer with nonylphenyl group as a side chain, was set in a vertically-stacked microreactor. The microreactor was set in a newly-developed automatic fluidic and detection system in a 25cm-cubic case and competitive ELISA was performed using this small system. As a result, we could construct a good standard curve with negative correlation in a concentration range of 0.01-1.00 microgram/l and the average coefficient of variation of the data obtained was 4.2%. These results were comparable to the sensitivity and repeatability of commercial sensitive analytical machines.
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