| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Ubiquitin chains with distinct linkage pattern are said to give specific biological functions to their substrate proteins. To understand these relationships between “linkages and functions”, we tried to identify ubiquitinated proteins with specific chain linkage patterns by modifying our original enrichment method for polyubiquitinated substrates. As result, we succeeded in identifying the linkage specific substrates for lys48 and lys63, which are main linkage points and already extensively characterized, but not for minor linkage points, which are major targets of this study.
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