Deduction of minimum sysmbiosis island gene sets for Lotus rhizobia

• Project/Area Number: 23510235
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Genome biology
• Research Institution: Nara Women's University
• Principal Investigator: SAEKI Kazuhiko 奈良女子大学, 自然科学系, 教授 (40201511)
• Co-Investigator(Renkei-kenkyūsha): SATO Shusei 東北大学, 生命科学研究科, 准教授 (70370921)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 植物微生物相互作用 / 共生 / 窒素固定 / 根粒形成 / 遺伝子水平伝搬

Research Abstract

In an attempt to deduce the rhizobial minimum symbiosis gene sets for mutualism between Mesorhizobium loti and Lotus species, we compared nucleotide sequences of symbiosis islands of three M. loti strains and found 155 genes are completely conserved among them. We made controllable promoters and new selectable markers to be used with M. loti strains. We also made a temperature sensitive derivative of the broad-host range vector pBBR1MCS2, by mutagenesis of its rep gene. In addition, we performed Next Generation Sequencing analysis of several M. loti strains and mapped the obtained reads on the genome sequence of the strain MAFF303099. The results supported the divergence of main chromosomes among M. loti strains with conserved core structure of symbiosis island.

Research Products

• [Journal Article] LjMATE1 : a citrate transporter responsible for iron supply to the nodule infection zone of Lotus japonicus (2013)
  • Author(s): Takanashi K, Yokosho K, Saeki K, Sugiyama A, Sato S, Tabata S, Ma JF and Yazaki K
  • Journal Title: Plant and Cell Physiology Volume: 54(4) Pages: 585-594
  • NAID: 10031163850
  • Peer Reviewed
• [Journal Article] Commonalities and Differences among Symbiosis Islands of Three <i>Mesorhizobium loti</i> Strains (2013)
  • Author(s): Kasai-Maita, H, Hirakawa H, Nakamura Y, Kaneko, T, Miki K, Maruya J, Okazaki S, Tabata S, Saeki K and Sato S
  • Journal Title: Microbes and Environments Volume: 28 Issue: 2 Pages: 275-278
  • DOI: 10.1264/jsme2.ME12201
  • NAID: 10031158633
  • ISSN: 1342-6311, 1347-4405
  • Peer Reviewed
• [Presentation] Core gene sets and evolution of symbiosis islands in rhizobial isolates from Lotus japonicus indigenous to Japan (2013)
  • Author(s): Saeki K, Kasai-Maita H, Kubota K, Kaneko T, Hirakawa H, Tabata S, Sato S
  • Organizer: 18th International Congress on Nitrogen Fixation, Phoenix Seagaia Resort ; World Convention Center Summit
  • Place of Presentation: Miyazaki, Japan
• [Presentation] ミヤコグサ根粒菌野生系統の耐塩性について (2013)
  • Author(s): 窪田和奈, 眞板寛子, 平川英樹, 佐藤修正, 佐伯和彦
  • Organizer: 植物微生物研究会・第23回研究交流会
  • Place of Presentation: 岡崎コンファレンスセンター(愛知県岡崎市)
• [Presentation] ミヤコグサ根粒菌(Mesorhizobium loti)12株における遺伝子構造の比較解析 (2013)
  • Author(s): 眞板寛子、平川英樹、佐伯和彦、田畑哲之、佐藤修正
  • Organizer: 第6回日本ゲノム微生物学会年会
  • Place of Presentation: 立教大学池袋キャンパス
• [Presentation] Core gene sets and evolution of symbiosis islands in rhizobial isolates from Lotus japonicas indigenous to Japan (2013)
  • Author(s): Kazuhiko Saeki, Hiroko Kasai-Maita, Kazuna Kubota, Takakazu Kaneko, Hideki Hirakawa, Satoshi Tabata, Shusei Sato
  • Organizer: 18th International Congress on Nitrogen Fixation
  • Place of Presentation: Phoenix Seagaia Resort; World Convention Center Summit, Miyazaki, Japan
• [Presentation] ミヤコグサ根粒菌野生系統の耐塩性について (2013)
  • Author(s): 窪田和奈, 眞板寛子, 平川英樹, 佐藤修正, 佐伯和彦
  • Organizer: 植物微生物研究会・第23回研究交流会
  • Place of Presentation: 岡崎コンファレンスセンター（愛知県岡崎市）
• [Presentation] ミヤコグサ根粒菌を宿主とした人為制御可能な発現ベクターの開発と応用 (2012)
  • Author(s): 白井理恵、中村公美,副島麻衣,岡崎伸、佐伯和彦
  • Organizer: 植物微生物研究会・第22回研究交流会
  • Place of Presentation: 神戸大学百年記念館
• [Presentation] ミヤコグサ根粒菌共生アイランドの伝搬と進化 : 宿主モデル系統 MG20自生地採集株群に焦点をあてて (2012)
  • Author(s): 佐伯和彦、谷佳美、池田翠,金子貴一,眞板寛子,平川英樹,田畑哲之、佐藤修正
  • Organizer: 植物微生物研究会・第22回研究交流会
  • Place of Presentation: 神戸大学百年記念館
• [Presentation] ミヤコグサ根粒菌共生アイランドの伝搬と進化：宿主モデル系統MG20 自生地採集株群に焦点をあてて (2012)
  • Author(s): 佐伯和彦、谷佳美、池田翠，金子貴一，眞板寛子，平川英樹，田畑哲之、佐藤修正
  • Organizer: 植物微生物研究会・第22回研究交流会
  • Place of Presentation: 神戸大学百年記念館
• [Presentation] ミヤコグサ根粒菌(Mesorhizobium loti) 12株における遺伝子構造の比較解析 (2012)
  • Author(s): 眞板寛子、平川英樹、佐伯和彦、田畑哲之、佐藤修正
  • Organizer: 第6回日本ゲノム微生物学会年会
  • Place of Presentation: 立教大学池袋キャンパス