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A Mechanism for Mitochondrial DNA Copy Number-regulated Simultaneous Expression of Proteins in Respiratory Complexes.

Research Project

Project/Area Number 23510237
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Genome biology
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

LING Feng  独立行政法人理化学研究所, 吉田化学遺伝学研究室, 専任研究員 (70281665)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords出芽酵母 / DNA組換え / ミトコンドリアDNA / ミトコンドリア融合 / 酸化ストレス応答 / ローリングサークル型複製 / DNA損傷 / Mhr1タンク質 / コンカテマー / DNA二本鎖切断 / 呼吸鎖複合体 / ミトコンドリア / ローリングサークル型DNA複製 / ミトコンドリアヌクレアーゼ / Saccharomyces cerevisiae / mitochondrial fusion / replication / zygote / Concatemer / Rolling-circle type / 遺伝学 / 遺伝子 / 核酸 / ストレス / 品質管理 / DNA複製
Research Abstract

We found that mitochondrial fusion induces dissociation of Complex V of the respiratory chain and ROS generation, which increases mitochondrial DNA (mtDNA) copy number in a manner dependent on the homologous DNA pairing protein Mhr1 and also increases in the mtDNA-encoded subunits of Complex V. These results suggested that activation of recombination-dependent replication during mitochondrial fusion plays important roles in maintenance of mitochondrial genomes by preventing generation of mitochondria lacking mtDNA. We further uncovered a mechanism in which relative expression levels of the DNA damage inducible protein Din7 to Mhr1 determine the choice of replication or repair after pairing of homologous DNA by Mhr1.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (18 results)

All 2014 2013 2012 2011 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (9 results) (of which Invited: 3 results)

  • [Journal Article] Din7 and Mhr1 expression levels regulate double-strand-break-induced replication and recombination of mtDNA at ori5 in yeast2013

    • Author(s)
      Feng Ling, Akiko Hori, Ayako Yoshitani, Rong Niu, Minoru Yoshida, and Takehiko Shibata
    • Journal Title

      Nucleic Acids Research

      Pages: 5799-5816

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Din7 and Mhr1 expression levels regulate double-strand-break–induced replication and recombination of mtDNA at ori5 in yeast2013

    • Author(s)
      Feng Ling, Akiko Hori, Ayako Yoshitani, Rong Niu, Minoru Yoshida and Takehiko Shibata,
    • Journal Title

      Nucleic Acids Research

      Volume: 41 (11) Pages: 5799-5816

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Din7 and Mhr1 expression levels regulate double-strand-break-induced replication and recombination of mtDNA at ori5 in yeast2013

    • Author(s)
      *Feng Ling, Akiko Hori, Ayako Yoshitani, Rong Niu, Minoru Yoshida and Takehiko Shibata
    • Journal Title

      Nucleic Acids Research

      Volume: 41(8) Issue: 11 Pages: 5799-5816

    • DOI

      10.1093/nar/gkt273

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mitochondrial fission proteins Fis1 and Mdv1, but not Dnm1, play a role in maintenance of heteroplasmy in budding yeast2012

    • Author(s)
      Elliot Bradshaw, Minoru Yoshida and Feng Ling
    • Journal Title

      FEBS Letters

      Volume: 586(8) Pages: 1245-1251

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Increased 4977-bp deletion in mitochondrial DNA upon ATM/Chk2 checkpoint activation in HeLa cells2012

    • Author(s)
      Rong Niu, Minoru Yoshida and Feng Ling
    • Journal Title

      PLoS One

      Volume: 7

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Increases in Mitochondrial DNA Content and 4977-bp Deletion Upon ATM/Chk2 Checkpoint Activation in HeLa Cells2012

    • Author(s)
      Rong Niu, Minoru Yoshida and *Feng Ling
    • Journal Title

      PLoS ONE

      Volume: 7(7)

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mitochondrial fusion increases the mitochondrial DNA copy number in budding yeast2011

    • Author(s)
      Akiko Hori, Minoru Yoshida and Feng Ling
    • Journal Title

      Genes to Cells

      Volume: 16 Pages: 527-544

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Gene conversion through double-strand break-induced DNA replication and mitochondrial homoplasmy2011

    • Author(s)
      Feng Ling, Tsutomu Mikawa and Takehiko Shibata
    • Journal Title

      Genes

      Volume: 2 Pages: 169-190

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enlightenment of yeast mitochondrial homoplasmy : diversified roles of gene conversion2011

    • Author(s)
      Ling, F., Mikawa, T. and Shibata, T.
    • Journal Title

      Genes (Basel)

      Volume: 2 Issue: 1 Pages: 169-190

    • DOI

      10.3390/genes2010169

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 健全な iPS 細胞樹立に向けたミトコンドリア病患者細胞の mtDNA ホモプラスミー化促進法の開発2014

    • Author(s)
      凌楓、牛栄、柴田武彦、吉田稔、畠山英之、後藤雄一
    • Organizer
      CREST/さきがけ/JST-CIRM 合同シンポジウム
    • Place of Presentation
      東京都江東区青海2-3-6 日本科学未来館
    • Year and Date
      2014-01-15
    • Related Report
      2013 Final Research Report
  • [Presentation] A Novel Mechanism for Mitochondrial DNA Inheritance and its Medical Significance2012

    • Author(s)
      Feng Ling
    • Organizer
      Mitochondrial Genetics Forum at Peking University
    • Place of Presentation
      Peking University First Hospital, Xi-shi-ku St, Beijing 100034, China
    • Year and Date
      2012-08-29
    • Related Report
      2013 Final Research Report
  • [Presentation] Role of Recombination-mediated Replication in Mitochondrial Inheritance2012

    • Author(s)
      Feng Ling
    • Organizer
      The Glycobiology Forum at Jiang Nan University
    • Place of Presentation
      School of Biotechnology, Jiangnan University, No. 188800 Lihu Avenue, Wuxi, Jiangsu, P. C. 214122, China
    • Year and Date
      2012-07-10
    • Related Report
      2013 Final Research Report
  • [Presentation] Recombination-mediated Replication in Mitochondrial DNA Inheritance2012

    • Author(s)
      Feng Ling, Rong Niu, Eliot Bradshaw, Minoru Yoshida
    • Organizer
      Global Conference of Chinese Geneticists
    • Place of Presentation
      Zhejiang University in Hangzhou, Zhejiang Province, China
    • Related Report
      2013 Final Research Report
  • [Presentation] A Novel Mechanism for Mitochondrial Inheritance & Its Biomedical Importance2012

    • Author(s)
      Feng Ling
    • Organizer
      Symposium of Mitochondral Biomedicine
    • Place of Presentation
      Zhejiang University in Hangzhou, Zhejiang Province, China
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] Recombination-mediated Replication in Mitochondrial DNA Inheritance2012

    • Author(s)
      ○Feng Ling, Rong Niu, Eliot Bradshaw, Minoru Yoshida
    • Organizer
      Global Conference of Chinese Geneticists,
    • Place of Presentation
      Zhejiang University in Hangzhou, Zhejiang Province, China
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 健全なiPS細胞樹立に向けたミトコンドリア病患者細胞のmtDNAホモプラスミー化促進法の開発

    • Author(s)
      凌楓、牛栄、柴田武彦、吉田稔、畠山英之、後藤雄一
    • Organizer
      CREST/さきがけ/JST-CIRM 合同シンポジウム
    • Place of Presentation
      東京都江東区青海2-3-6 日本科学未来館
    • Related Report
      2013 Annual Research Report
  • [Presentation] Role of Recombination-mediated Replication in Mitochondrial Inheritance

    • Author(s)
      Feng Ling
    • Organizer
      The Glycobiology Forum at Jiang Nan University, School of Biotechnology, Jiangnan University,
    • Place of Presentation
      No. 188800 Lihu Avenue, Wuxi, Jiangsu, P.C. 214122, China
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] A Novel Mechanism for Mitochondrial DNA Inheritance and its Medical Significanc

    • Author(s)
      Feng Ling
    • Organizer
      Mitochondrial Genetics Forum at Peking University
    • Place of Presentation
      Peking University First Hospital, Xi-shi-ku St, Beijing 100034, China
    • Related Report
      2012 Research-status Report
    • Invited

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Published: 2011-08-05   Modified: 2019-07-29  

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