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Survey of anti-desiccation and ant-freezing genes in Antarctic nematodes by comparative genome analysis

Research Project

Project/Area Number 23510239
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Genome biology
Research Institution大学共同利用機関法人情報・システム研究機構(新領域融合研究センター及びライフサイ

Principal Investigator

KAGOSHIMA Hiroshi  大学共同利用機関法人情報・システム研究機構(新領域融合研究センター及びライフサイ, 大学共同利用機関等の部局等, プロジェクト特任研究員 (00550063)

Co-Investigator(Renkei-kenkyūsha) TOYODA Atsushi   (10267495)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords比較ゲノム / 極限環境 / 乾燥耐性 / 凍結耐性 / 線形動物 / 緩歩動物 / 南極 / 国際情報交換(ニュージーランド、英国) / 乾燥耐性遺伝子 / 凍結耐性遺伝子 / 線虫 / クマムシ / 国際情報交換(イギリス:ニュージーランド) / 環境耐性遺伝子 / 国際情報交流 / イギリス:ニュージーランド
Research Abstract

The Antarctic nematode, Panagrolaimus davidi, is a remarkable organism that tolerates desiccation and intracellular freezing. To elucidate responsible genes for the tolerances, I have performed large-scale transcriptome and genome analyses in collaboration with the researchers in UK and New Zealand, and identified 25,875 transcripts. We picked up a candidate gene family for the tolerances, LEA genes, which are reported only from organisms living in extreme environments. Although even these special organisms have one or a few LEA genes normally, P. davidi has excess number of (at least 14 types of) LEA genes. Promotion of this research may facilitate the application to preserve internal organs and living bodies in frozen and/or dry state.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (38 results)

All 2014 2013 2012 2011 Other

All Journal Article (14 results) (of which Peer Reviewed: 14 results) Presentation (23 results) (of which Invited: 3 results) Remarks (1 results)

  • [Journal Article] Co-expression of the transcription factors CEH-14 and TTX-1 regulates AFD neuron-specific genes gcy-8 and gcy-18 in C. elegans2014

    • Author(s)
      Kagoshima H and Kohara Y
    • Journal Title

      Dev Biol

      Volume: (Accepted with minor revision)

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular analysis of the cold tolerant Antarctic nematode2014

    • Author(s)
      Thorne M, Kagoshima H, Clark M, Marshall C and Wharton D
    • Journal Title

      Panagrolaimus davidi, PLoS ONE

      Volume: (Accepted with minor revision)

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The LIM homeobox gene ceh-14 regulates neurite outgrowth in select neurons.2013

    • Author(s)
      Kagoshima H, Cassata G, Tong YG, Pujol N, Niklaus G, Burglin TR
    • Journal Title

      Developmental Biology

      Volume: 380 Issue: 2 Pages: 314-323

    • DOI

      10.1016/j.ydbio.2013.04.009

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular and morphological analysis of Antarctic tardigrade, Acutuncus antarcticus2013

    • Author(s)
      Kagoshima, H, Imura, S and Suzuki, AC
    • Journal Title

      Journal of Limnology

      Volume: 72(s1) Issue: 1s Pages: 15-23

    • DOI

      10.4081/jlimnol.2013.s1.e3

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 南極生物における凍結・乾燥耐性研究の現状2013

    • Author(s)
      鹿児島 浩
    • Journal Title

      極限生物学会誌

      Volume: Vol. 12, No 2 Pages: 71-78

    • NAID

      40020070876

    • URL

      http://www.extremophiles.jp/gakkaishi.html

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Studies on anti-desiccation and anti-freezng mechanisms in Antarctic organisms.2013

    • Author(s)
      Kagoshima H
    • Journal Title

      Journal of Japanese Society for Extremophiles

      Volume: 12 Pages: 71-78

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The LIM homeobox gene ceh-14 regulates neurite outgrowth in select neurons.2013

    • Author(s)
      Kagoshima H, Cassata G, Tong YG, Pujol N, Niklaus G, Burglin TR.
    • Journal Title

      Developmenta Biology

      Volume: in press

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Antarctic tardigrade culture from moss samples near Syowa Station, East Antarctica.2013

    • Author(s)
      Kagoshima H, Imura S, Suzuki AC.
    • Journal Title

      Journal of Limnology

      Volume: 72(s1) Pages: 15-23

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Multi-decadal survival of an Antarctic nematode, Plectus murrayi, in a -20℃ stored moss sample2012

    • Author(s)
      Kagoshima H, Kito K, Aizu T, Shin-i T, Kanda H, Kobayashi S, Toyoda A, Fujiyama A, Kohara Y, Convey P, Niki H
    • Journal Title

      Cryo Letters

      Volume: 33(4) Pages: 280-288

    • URL

      http://www.cryoletters.org/Abstracts/vol_33_4_2012.htm#280

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Diversity of RuBisCO gene responsible for CO_2 fixation in an Antarcticmoss pillar.2012

    • Author(s)
      Nakai R, Abe T, Baba T, Imura S, Kagoshima H, Kanda H, Kohara Y, Koi A, Niki H, Yanagihara K, Naganuma T
    • Journal Title

      Polar Biology

      Volume: 35 Issue: 11 Pages: 1641-1650

    • DOI

      10.1007/s00300-012-1204-5

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Eukaryotic phylotypes in aquatic moss pillars inhabiting a freshwater lake in East Antarctica, based on 18S rRNA gene analysis.2012

    • Author(s)
      Nakai R, Abe T, Baba T, Imura S, Kagoshima H, Kanda H, Kohara Y, Koi A, Niki H, Yanagihara K, Naganuma T
    • Journal Title

      Polar Biology

      Volume: 35 Issue: 10 Pages: 1495-1504

    • DOI

      10.1007/s00300-012-1188-1

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Microflorae of aquatic moss pillars in a freshwater lake, East Antarctica, based on fatty acid and 16S rRNA gene analyses2012

    • Author(s)
      Ryosuke Nakai, Takashi Abe, Tomoya Baba, Satoshi Imura, Hiroshi Kagoshima, Hiroshi Kanda, Atsuko Kanekiyo, Yuji Kohara, Akiko Koi, Keiko Nakamura, Takanori Narita, Hironori Niki, Katsuhiko Yanagihara, Takeshi Naganuma
    • Journal Title

      Polar Biology

      Volume: 35 Issue: 3 Pages: 425-433

    • DOI

      10.1007/s00300-011-1090-2

    • Related Report
      2013 Final Research Report 2012 Research-status Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Multi-decadal survival of an Antarctic nematode, Plectus murrayi, in a -20°C stored moss sample.2012

    • Author(s)
      Kagoshima H, Kito K, Aizu T, Shin-i T, Kanda H, Kobayashi S, Toyoda A, Fujiyama A, Kohara Y, Convey P, Niki H.
    • Journal Title

      Cryo Letters

      Volume: 33(4) Pages: 280-288

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Eukaryotic phylotypes in aquatic moss pillars inhabiting a freshwater lake in East Antarctica, based on 18S rRNA gene analysis.2012

    • Author(s)
      Nakai, R., Abe, T., Baba, T., Imura, S., Kagoshima, H., Kanda, H., Kohara, Y., Koi, A., Niki, H., Yanagihara, K. and Naganuma, T.
    • Journal Title

      Polar Biology

      Volume: -

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 南極線虫 Panagrolaimus davidi の乾燥耐性遺伝子の機能解析2013

    • Author(s)
      鹿児島 浩、小原 雄治、仁木 宏典
    • Organizer
      日本分子生物学会第36回年会
    • Place of Presentation
      ポートピアホテル
    • Year and Date
      2013-12-03
    • Related Report
      2013 Final Research Report
  • [Presentation] Genome and transcriptome analysis of the parthenogenetic nematode Diploscapter coronatus2013

    • Author(s)
      Hiraki H, Kagoshima H, Ueta Y, Kraus C, Schiffer P, Schierenberg E, Kohara Y
    • Organizer
      日本分子生物学会第36回年会
    • Place of Presentation
      ポートピアホテル
    • Year and Date
      2013-12-03
    • Related Report
      2013 Final Research Report
  • [Presentation] Functional analysis of anti-desiccation gene in Antarctic nematode Panagrolaimus davidi2013

    • Author(s)
      Kagoshima H, Kohara Y, Niki H
    • Organizer
      The Fourth Symposium on Polar Science
    • Place of Presentation
      国立極地研究所
    • Year and Date
      2013-11-12
    • Related Report
      2013 Final Research Report
  • [Presentation] Panagrolaimus davidi の乾燥耐性タンパク質の機能解析2013

    • Author(s)
      鹿児島 浩、小原 雄治、仁木 宏典. 南極線虫
    • Organizer
      第14回極限環境生物学会シンポジウム
    • Place of Presentation
      東洋大学白山キャンパス
    • Year and Date
      2013-06-15
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] The homeobox transcription factors, CEH-14 and TTX-1 regulate the AFD neuron specific gene expression of gcy-8 and gcy-18 in C. elegans2013

    • Author(s)
      Kagoshima H, Kohara Y
    • Organizer
      19th International C. elegans Meeting
    • Place of Presentation
      UCLA, Los Angeles, USA
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] LEA genes in Antarctic nematode, Panagrolaimus davidi2013

    • Author(s)
      Kagoshima H, Kohara Y
    • Organizer
      19th International C. elegans Meeting
    • Place of Presentation
      UCLA, Los Angeles, USA
    • Related Report
      2013 Annual Research Report 2013 Final Research Report 2012 Research-status Report
  • [Presentation] 南極線虫Panagrolaimus davidiの乾燥耐性タンパク質の機能解析2013

    • Author(s)
      鹿児島浩、小原雄治、仁木宏典
    • Organizer
      第14回極限環境生物学会シンポジウム
    • Place of Presentation
      東洋大学、白山キャンパス、東京
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] The homeobox transcription factors, CEH-14 and TTX-1 regulate the AFD neuron-specific gene expression of gcy-8 and gcy-18 in C. elegans.2013

    • Author(s)
      Kagoshima H, Kohara Y
    • Organizer
      19th International C. elegans Meeting
    • Place of Presentation
      UCLA, Los Angeles, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] Functional analysis of anti-desiccation gene in Antarctic nematode Panagrolaimus davidi.2013

    • Author(s)
      Kagoshima H, Kohara Y, Niki H
    • Organizer
      The Fourth Symposium on Polar Science
    • Place of Presentation
      国立極地研究所、東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] Genome and transcriptome analysis of the parthenogenetic nematode Diploscapter coronatus2013

    • Author(s)
      Hiraki H, Kagoshima H, Ueta Y, Kraus C, Schiffer P, Schierenberg E, Kohara Y
    • Organizer
      日本分子生物学会第36回年会
    • Place of Presentation
      ポートピアホテル、兵庫
    • Related Report
      2013 Annual Research Report
  • [Presentation] 南極線虫Panagrolaimus davidi の乾燥耐性遺伝子の機能解析2013

    • Author(s)
      鹿児島 浩、小原 雄治、仁木 宏典
    • Organizer
      日本分子生物学会第36回年会
    • Place of Presentation
      ポートピアホテル、兵庫
    • Related Report
      2013 Annual Research Report
  • [Presentation] 南極線虫Panagrolaimus davidiの乾燥耐性タンパク質の機能解析2013

    • Author(s)
      鹿児島 浩、小原 雄治、仁木 宏典
    • Organizer
      第14回 極限環境生物学会シンポジウム
    • Place of Presentation
      東洋大学 白山キャンパス
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] The homeobox transcription factors, CEH-14 and TTX-1, regulate AFD thermosensory neuron-specific expression of gcy-8 and gcy-18 in C. elegans2012

    • Author(s)
      Kagoshima H, Kohara Y
    • Organizer
      日本分子生物学会第35回年会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡
    • Year and Date
      2012-12-12
    • Related Report
      2013 Final Research Report
  • [Presentation] Functional analysis of anti-desiccation proteins from Antarctic nematode, Panagrolaimus davidi, and Japanese anhydrobiotic tardigrade, Ramazzottius varieornatus2012

    • Author(s)
      Kagoshima H, Kunieda T
    • Organizer
      第34回極域生物シンポジウム
    • Place of Presentation
      国立極地研究所
    • Year and Date
      2012-11-27
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] The homeobox transcription factors, CEH-14 and TTX-1, regulate AFD thermosensory neuron-specific expression of gcy-8 and gcy-18 in C. elegans.2012

    • Author(s)
      Kagoshima H, Kohara Y
    • Organizer
      日本分子生物学会 第35回年会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡
    • Related Report
      2012 Research-status Report
  • [Presentation] The homeobox transcription factors, CEH-14 and TTX-1, regulate AFD thermosensory neuron-specific expression of gcy-8 and gcy-18 in C. elegans2011

    • Author(s)
      Kagoshima H and Kohara Y
    • Organizer
      日本分子生物学会第34回年会
    • Place of Presentation
      横浜パシフィコ
    • Year and Date
      2011-12-15
    • Related Report
      2013 Final Research Report
  • [Presentation] Multi-decadal survival of an antarctic nematode, Plectus murrayi, in a -20°C stored moss sample2011

    • Author(s)
      Kagoshima H
    • Organizer
      第33回極域生物シンポジウム
    • Place of Presentation
      国立極地研究所
    • Year and Date
      2011-11-18
    • Related Report
      2013 Final Research Report
  • [Presentation] Functional genomics of two Antarctic nematodes, Panagrolaimus davidi and Plectus murrayi2011

    • Author(s)
      Kagoshima H and Kohara Y
    • Organizer
      18th Intrnational C. elegans Meeting
    • Place of Presentation
      University of California, Los Angeles, USA
    • Year and Date
      2011-06-24
    • Related Report
      2013 Final Research Report
  • [Presentation] The homeobox transcription factors, CEH-14 and TTX-1, regulate expression of gcy-8 and gcy-18 in C. elegans2011

    • Author(s)
      Kagoshima H and Kohara Y
    • Organizer
      18th Intrnational C. elegans Meeting
    • Place of Presentation
      University of California, Los Angeles, USA
    • Year and Date
      2011-06-24
    • Related Report
      2013 Final Research Report
  • [Presentation] The homeobox transcription factors, CEH-14 and TTX-1, regulate expression of gcy-8 and gcy-18 in C. elegans.2011

    • Author(s)
      Kagoshima, H. and Kohara, Y.
    • Organizer
      18th Intrnational C. elegans Meeting
    • Place of Presentation
      University of California, Los Angeles, USA
    • Related Report
      2011 Research-status Report
  • [Presentation] Functional genomics of two Antarctic nematodes, Panagrolaimus davidi and Plectus murrayi.2011

    • Author(s)
      Kagoshima, H. and Kohara, Y.
    • Organizer
      18th Intrnational C. elegans Meeting
    • Place of Presentation
      University of California, Los Angeles, USA
    • Related Report
      2011 Research-status Report
  • [Presentation] The homeobox transcription factors, CEH-14 and TTX-1, regulate AFD thermosensory neuron-specific expression of gcy-8 and gcy-18 in C. elegans.2011

    • Author(s)
      Kagoshima, H. and Kohara, Y.
    • Organizer
      日本分子生物学会第34回年会
    • Place of Presentation
      横浜パシフィコ
    • Related Report
      2011 Research-status Report
  • [Presentation] Multi-decadal survival of an antarctic nematode, Plectus murrayi, in a -20°C stored moss sample.2011

    • Author(s)
      Kagoshima, H.
    • Organizer
      第33回極域生物シンポジウム
    • Place of Presentation
      国立極地研究所
    • Related Report
      2011 Research-status Report
  • [Remarks] ホームページ等 線虫 C. elegans プロモーター配列データベース代表者

    • URL

      http://www.shigen.nig.ac.jp/c.elegans/promoter/index.jsp

    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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