Survey of anti-desiccation and ant-freezing genes in Antarctic nematodes by comparative genome analysis

• Project/Area Number: 23510239
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Genome biology
• Research Institution: 大学共同利用機関法人情報・システム研究機構(新領域融合研究センター及びライフサイ
• Principal Investigator: KAGOSHIMA Hiroshi 大学共同利用機関法人情報・システム研究機構(新領域融合研究センター及びライフサイ, 大学共同利用機関等の部局等, プロジェクト特任研究員 (00550063)
• Co-Investigator(Renkei-kenkyūsha): TOYODA Atsushi (10267495)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 比較ゲノム / 極限環境 / 乾燥耐性 / 凍結耐性 / 線形動物 / 緩歩動物 / 南極 / 国際情報交換（ニュージーランド、英国） / 乾燥耐性遺伝子 / 凍結耐性遺伝子 / 線虫 / クマムシ / 国際情報交換（イギリス：ニュージーランド） / 環境耐性遺伝子 / 国際情報交流 / イギリス：ニュージーランド

Research Abstract

The Antarctic nematode, Panagrolaimus davidi, is a remarkable organism that tolerates desiccation and intracellular freezing. To elucidate responsible genes for the tolerances, I have performed large-scale transcriptome and genome analyses in collaboration with the researchers in UK and New Zealand, and identified 25,875 transcripts. We picked up a candidate gene family for the tolerances, LEA genes, which are reported only from organisms living in extreme environments. Although even these special organisms have one or a few LEA genes normally, P. davidi has excess number of (at least 14 types of) LEA genes. Promotion of this research may facilitate the application to preserve internal organs and living bodies in frozen and/or dry state.

Research Products

• [Journal Article] Co-expression of the transcription factors CEH-14 and TTX-1 regulates AFD neuron-specific genes gcy-8 and gcy-18 in C. elegans (2014)
  • Author(s): Kagoshima H and Kohara Y
  • Journal Title: Dev Biol Volume: (Accepted with minor revision)
  • Peer Reviewed
• [Journal Article] Molecular analysis of the cold tolerant Antarctic nematode (2014)
  • Author(s): Thorne M, Kagoshima H, Clark M, Marshall C and Wharton D
  • Journal Title: Panagrolaimus davidi, PLoS ONE Volume: (Accepted with minor revision)
  • Peer Reviewed
• [Journal Article] The LIM homeobox gene ceh-14 regulates neurite outgrowth in select neurons. (2013)
  • Author(s): Kagoshima H, Cassata G, Tong YG, Pujol N, Niklaus G, Burglin TR
  • Journal Title: Developmental Biology Volume: 380 Issue: 2 Pages: 314-323
  • DOI: 10.1016/j.ydbio.2013.04.009
  • Peer Reviewed
• [Journal Article] Molecular and morphological analysis of Antarctic tardigrade, Acutuncus antarcticus (2013)
  • Author(s): Kagoshima, H, Imura, S and Suzuki, AC
  • Journal Title: Journal of Limnology Volume: 72(s1) Issue: 1s Pages: 15-23
  • DOI: 10.4081/jlimnol.2013.s1.e3
  • Peer Reviewed
• [Journal Article] 南極生物における凍結・乾燥耐性研究の現状 (2013)
  • Author(s): 鹿児島 浩
  • Journal Title: 極限生物学会誌 Volume: Vol. 12, No 2 Pages: 71-78
  • NAID: 40020070876
  • URL: http://www.extremophiles.jp/gakkaishi.html
  • Peer Reviewed
• [Journal Article] Studies on anti-desiccation and anti-freezng mechanisms in Antarctic organisms. (2013)
  • Author(s): Kagoshima H
  • Journal Title: Journal of Japanese Society for Extremophiles Volume: 12 Pages: 71-78
  • Peer Reviewed
• [Journal Article] The LIM homeobox gene ceh-14 regulates neurite outgrowth in select neurons. (2013)
  • Author(s): Kagoshima H, Cassata G, Tong YG, Pujol N, Niklaus G, Burglin TR.
  • Journal Title: Developmenta Biology Volume: in press
  • Peer Reviewed
• [Journal Article] Antarctic tardigrade culture from moss samples near Syowa Station, East Antarctica. (2013)
  • Author(s): Kagoshima H, Imura S, Suzuki AC.
  • Journal Title: Journal of Limnology Volume: 72(s1) Pages: 15-23
  • Peer Reviewed
• [Journal Article] Multi-decadal survival of an Antarctic nematode, Plectus murrayi, in a -20℃ stored moss sample (2012)
  • Author(s): Kagoshima H, Kito K, Aizu T, Shin-i T, Kanda H, Kobayashi S, Toyoda A, Fujiyama A, Kohara Y, Convey P, Niki H
  • Journal Title: Cryo Letters Volume: 33(4) Pages: 280-288
  • URL: http://www.cryoletters.org/Abstracts/vol_33_4_2012.htm#280
  • Peer Reviewed
• [Journal Article] Diversity of RuBisCO gene responsible for CO_2 fixation in an Antarcticmoss pillar. (2012)
  • Author(s): Nakai R, Abe T, Baba T, Imura S, Kagoshima H, Kanda H, Kohara Y, Koi A, Niki H, Yanagihara K, Naganuma T
  • Journal Title: Polar Biology Volume: 35 Issue: 11 Pages: 1641-1650
  • DOI: 10.1007/s00300-012-1204-5
  • Peer Reviewed
• [Journal Article] Eukaryotic phylotypes in aquatic moss pillars inhabiting a freshwater lake in East Antarctica, based on 18S rRNA gene analysis. (2012)
  • Author(s): Nakai R, Abe T, Baba T, Imura S, Kagoshima H, Kanda H, Kohara Y, Koi A, Niki H, Yanagihara K, Naganuma T
  • Journal Title: Polar Biology Volume: 35 Issue: 10 Pages: 1495-1504
  • DOI: 10.1007/s00300-012-1188-1
  • Peer Reviewed
• [Journal Article] Microflorae of aquatic moss pillars in a freshwater lake, East Antarctica, based on fatty acid and 16S rRNA gene analyses (2012)
  • Author(s): Ryosuke Nakai, Takashi Abe, Tomoya Baba, Satoshi Imura, Hiroshi Kagoshima, Hiroshi Kanda, Atsuko Kanekiyo, Yuji Kohara, Akiko Koi, Keiko Nakamura, Takanori Narita, Hironori Niki, Katsuhiko Yanagihara, Takeshi Naganuma
  • Journal Title: Polar Biology Volume: 35 Issue: 3 Pages: 425-433
  • DOI: 10.1007/s00300-011-1090-2
  • Peer Reviewed
• [Journal Article] Multi-decadal survival of an Antarctic nematode, Plectus murrayi, in a -20°C stored moss sample. (2012)
  • Author(s): Kagoshima H, Kito K, Aizu T, Shin-i T, Kanda H, Kobayashi S, Toyoda A, Fujiyama A, Kohara Y, Convey P, Niki H.
  • Journal Title: Cryo Letters Volume: 33(4) Pages: 280-288
  • Peer Reviewed
• [Journal Article] Eukaryotic phylotypes in aquatic moss pillars inhabiting a freshwater lake in East Antarctica, based on 18S rRNA gene analysis. (2012)
  • Author(s): Nakai, R., Abe, T., Baba, T., Imura, S., Kagoshima, H., Kanda, H., Kohara, Y., Koi, A., Niki, H., Yanagihara, K. and Naganuma, T.
  • Journal Title: Polar Biology Volume: -
  • Peer Reviewed
• [Presentation] 南極線虫 Panagrolaimus davidi の乾燥耐性遺伝子の機能解析 (2013)
  • Author(s): 鹿児島 浩、小原 雄治、仁木 宏典
  • Organizer: 日本分子生物学会第36回年会
  • Place of Presentation: ポートピアホテル
  • Year and Date: 2013-12-03
• [Presentation] Genome and transcriptome analysis of the parthenogenetic nematode Diploscapter coronatus (2013)
  • Author(s): Hiraki H, Kagoshima H, Ueta Y, Kraus C, Schiffer P, Schierenberg E, Kohara Y
  • Organizer: 日本分子生物学会第36回年会
  • Place of Presentation: ポートピアホテル
  • Year and Date: 2013-12-03
• [Presentation] Functional analysis of anti-desiccation gene in Antarctic nematode Panagrolaimus davidi (2013)
  • Author(s): Kagoshima H, Kohara Y, Niki H
  • Organizer: The Fourth Symposium on Polar Science
  • Place of Presentation: 国立極地研究所
  • Year and Date: 2013-11-12
• [Presentation] Panagrolaimus davidi の乾燥耐性タンパク質の機能解析 (2013)
  • Author(s): 鹿児島 浩、小原 雄治、仁木 宏典. 南極線虫
  • Organizer: 第14回極限環境生物学会シンポジウム
  • Place of Presentation: 東洋大学白山キャンパス
  • Year and Date: 2013-06-15
  • Invited
• [Presentation] The homeobox transcription factors, CEH-14 and TTX-1 regulate the AFD neuron specific gene expression of gcy-8 and gcy-18 in C. elegans (2013)
  • Author(s): Kagoshima H, Kohara Y
  • Organizer: 19th International C. elegans Meeting
  • Place of Presentation: UCLA, Los Angeles, USA
• [Presentation] LEA genes in Antarctic nematode, Panagrolaimus davidi (2013)
  • Author(s): Kagoshima H, Kohara Y
  • Organizer: 19th International C. elegans Meeting
  • Place of Presentation: UCLA, Los Angeles, USA
• [Presentation] 南極線虫Panagrolaimus davidiの乾燥耐性タンパク質の機能解析 (2013)
  • Author(s): 鹿児島浩、小原雄治、仁木宏典
  • Organizer: 第14回極限環境生物学会シンポジウム
  • Place of Presentation: 東洋大学、白山キャンパス、東京
  • Invited
• [Presentation] The homeobox transcription factors, CEH-14 and TTX-1 regulate the AFD neuron-specific gene expression of gcy-8 and gcy-18 in C. elegans. (2013)
  • Author(s): Kagoshima H, Kohara Y
  • Organizer: 19th International C. elegans Meeting
  • Place of Presentation: UCLA, Los Angeles, USA
• [Presentation] Functional analysis of anti-desiccation gene in Antarctic nematode Panagrolaimus davidi. (2013)
  • Author(s): Kagoshima H, Kohara Y, Niki H
  • Organizer: The Fourth Symposium on Polar Science
  • Place of Presentation: 国立極地研究所、東京
• [Presentation] Genome and transcriptome analysis of the parthenogenetic nematode Diploscapter coronatus (2013)
  • Author(s): Hiraki H, Kagoshima H, Ueta Y, Kraus C, Schiffer P, Schierenberg E, Kohara Y
  • Organizer: 日本分子生物学会第36回年会
  • Place of Presentation: ポートピアホテル、兵庫
• [Presentation] 南極線虫Panagrolaimus davidi の乾燥耐性遺伝子の機能解析 (2013)
  • Author(s): 鹿児島 浩、小原 雄治、仁木 宏典
  • Organizer: 日本分子生物学会第36回年会
  • Place of Presentation: ポートピアホテル、兵庫
• [Presentation] 南極線虫Panagrolaimus davidiの乾燥耐性タンパク質の機能解析 (2013)
  • Author(s): 鹿児島 浩、小原 雄治、仁木 宏典
  • Organizer: 第14回 極限環境生物学会シンポジウム
  • Place of Presentation: 東洋大学 白山キャンパス
  • Invited
• [Presentation] The homeobox transcription factors, CEH-14 and TTX-1, regulate AFD thermosensory neuron-specific expression of gcy-8 and gcy-18 in C. elegans (2012)
  • Author(s): Kagoshima H, Kohara Y
  • Organizer: 日本分子生物学会第35回年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡
  • Year and Date: 2012-12-12
• [Presentation] Functional analysis of anti-desiccation proteins from Antarctic nematode, Panagrolaimus davidi, and Japanese anhydrobiotic tardigrade, Ramazzottius varieornatus (2012)
  • Author(s): Kagoshima H, Kunieda T
  • Organizer: 第34回極域生物シンポジウム
  • Place of Presentation: 国立極地研究所
  • Year and Date: 2012-11-27
• [Presentation] The homeobox transcription factors, CEH-14 and TTX-1, regulate AFD thermosensory neuron-specific expression of gcy-8 and gcy-18 in C. elegans. (2012)
  • Author(s): Kagoshima H, Kohara Y
  • Organizer: 日本分子生物学会 第35回年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡
• [Presentation] The homeobox transcription factors, CEH-14 and TTX-1, regulate AFD thermosensory neuron-specific expression of gcy-8 and gcy-18 in C. elegans (2011)
  • Author(s): Kagoshima H and Kohara Y
  • Organizer: 日本分子生物学会第34回年会
  • Place of Presentation: 横浜パシフィコ
  • Year and Date: 2011-12-15
• [Presentation] Multi-decadal survival of an antarctic nematode, Plectus murrayi, in a -20°C stored moss sample (2011)
  • Author(s): Kagoshima H
  • Organizer: 第33回極域生物シンポジウム
  • Place of Presentation: 国立極地研究所
  • Year and Date: 2011-11-18
• [Presentation] Functional genomics of two Antarctic nematodes, Panagrolaimus davidi and Plectus murrayi (2011)
  • Author(s): Kagoshima H and Kohara Y
  • Organizer: 18th Intrnational C. elegans Meeting
  • Place of Presentation: University of California, Los Angeles, USA
  • Year and Date: 2011-06-24
• [Presentation] The homeobox transcription factors, CEH-14 and TTX-1, regulate expression of gcy-8 and gcy-18 in C. elegans (2011)
  • Author(s): Kagoshima H and Kohara Y
  • Organizer: 18th Intrnational C. elegans Meeting
  • Place of Presentation: University of California, Los Angeles, USA
  • Year and Date: 2011-06-24
• [Presentation] The homeobox transcription factors, CEH-14 and TTX-1, regulate expression of gcy-8 and gcy-18 in C. elegans. (2011)
  • Author(s): Kagoshima, H. and Kohara, Y.
  • Organizer: 18th Intrnational C. elegans Meeting
  • Place of Presentation: University of California, Los Angeles, USA
• [Presentation] Functional genomics of two Antarctic nematodes, Panagrolaimus davidi and Plectus murrayi. (2011)
  • Author(s): Kagoshima, H. and Kohara, Y.
  • Organizer: 18th Intrnational C. elegans Meeting
  • Place of Presentation: University of California, Los Angeles, USA
• [Presentation] The homeobox transcription factors, CEH-14 and TTX-1, regulate AFD thermosensory neuron-specific expression of gcy-8 and gcy-18 in C. elegans. (2011)
  • Author(s): Kagoshima, H. and Kohara, Y.
  • Organizer: 日本分子生物学会第34回年会
  • Place of Presentation: 横浜パシフィコ
• [Presentation] Multi-decadal survival of an antarctic nematode, Plectus murrayi, in a -20°C stored moss sample. (2011)
  • Author(s): Kagoshima, H.
  • Organizer: 第33回極域生物シンポジウム
  • Place of Presentation: 国立極地研究所
• [Remarks] ホームページ等 線虫 C. elegans プロモーター配列データベース代表者
  • URL: http://www.shigen.nig.ac.jp/c.elegans/promoter/index.jsp