Analyses of genomic structure of gene amplification regions in human cancer cells and disease causation
| Project/Area Number |
23510241
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Medical genome science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SATOH HITOSHI 東京大学, 新領域創成科学研究科, 准教授 (70183829)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 染色体 / FISH / 遺伝子増幅 / 癌 / ゲノム構造 / がん / ゲノム / 原発生体腔内リンパ腫 / 乳癌細胞株 / 肺癌細胞株 / 原発性体腔内リンパ腫 |
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| Outline of Final Research Achievements |
In general, it happened apparently that a variety of gene amplification and deletion may interfere its expression and regulation. In the present study, I applied FISH technique to detect how the NOTCH3 gene is amplified in a mammary gland ductal carcinoma cell line of HC1143 and to make it narrow down a possible amplified genomic region commonly found in two of cell lines derived from primary effusion lymphomas. As a result, amplification of NOTCH3 gene was certified in HC1143 cells and a possible amplified region was narrowed down within the 1q25 region in PEL cell lines. However, disease causing candidate genes are not yet identified.
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Report
(4 results)
Research Products
(7 results)
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[Journal Article] Isolation and characterization of mesenchymal stem cells from human umbilical cord blood: Reevaluation of critical factors for successful isolation and high ability to proliferate and differentiate to chondrocytes as compared to mesenchymal stem cells from bone marrow and adipose tissue.2011
Author(s)
Xiaohong Zhang, M Hirai, S Cantero, R Ciubotariu, L Dobrila, A Hirsh, K Igura, Hitoshi Satoh, I Yokomi, T Nishimura, S Yamaguchi, K Yoshimura, P Rubinstein, and Tsuneo A. Takahashi
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Journal Title
Journal of Cellular Biochemistry
Volume: 112
Issue: 4
Pages: 1206-1218
DOI
Related Report
Peer Reviewed
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