Responsible genes for the aberrant production of MPO-ANCA
| Project/Area Number |
23510244
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Medical genome science
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| Research Institution | Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology (2012-2014)
Jichi Medical University (2011) |
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Principal Investigator |
HAMANO Yoshitomo 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 東京都健康長寿医療センター研究所, 研究員 (10281354)
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | SCG/Kj / MPO-ANCA / 血管炎 / 半月体形成性糸球体腎炎 / マッピング / ANCA関連血管炎 / 自己免疫 / 自己免疫疾患 |
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| Outline of Final Research Achievements |
The spontaneous crescentic glomerulonephritis-forming/Kinjoh (SCG/Kj) mouse is a unique model of vasculitis with production of MPO-ANCA. Genome wide mapping revealed the non-Fas susceptibility locus of MPO-ANCA on chromosome 1, designated as Man-1. The aim of this study is to identify the responsible gene (s) of Man-1. Interval mapping created single peak of LOD curve on the Gene A, a protein-coding gene with unknown function. Because of the highest LOD score, Gene A was the first positional candidate gene. Recombinant Gene A accelerated the proliferation of murine myeloid cell line Mcl33D. However, transfection of Gene A to Mcl33D did not influenced to expression levels of leukocyte activation markers.
We established SCG/Kj-derived interval congenic mice with B6 background. Fortunately, we obtained mice with recombination between B6 and SCG/Kj at the midpoint of Man-1 interval. These mice will help us to narrow down positional candidate genes on Man-1 interval.
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Report
(5 results)
Research Products
(22 results)
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[Journal Article] Comparison of phenotype and outcome in microscopic polyangiitis between Europe and Japan2014
Author(s)
Furuta, S., Chaudhry, AN., Hamano, Y., Fujimoto, S., Nagafuchi, H., Makino, H., Matsuo, S., Ozaki, S., Endo, T., Muso, E., Ito, C., Kusano, E., Yamagata, M., Ikeda, K., Kashiwakuma, D., Iwamoto, I., Westman, K. and Jayne, D.
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Journal Title
J Rheumatol
Volume: 41(2)
Pages: 325-33
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] A novel autoantibody against moesin in the serum of patients with MPO-ANCA-associated vasculitis.2014
Author(s)
Suzuki K, Nagao T, Itabashi M, Hamano Y, Sugamata R, Yamazaki Y, Yumura W, Tsukita S, Wang PC, Nakayama T, Suzuki K
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Journal Title
Nephrol Dial Transplant.
Volume: 29
Pages: 1168-1177
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Susceptibility QTLs for pathogenic leukocytosis in SCG/Kj mice, a spontaneously occurring crescentic glomerulonephritis and vasculitis model.2014
Author(s)
Hamano Y, Abe M, Matsuoka S, Zhang D, Kondo Y, Kagami Y, Ishigami A, Maruyama N, Tsuruta Y, Yumura W, Suzuki K
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Journal Title
Clin Exp Immunol
Volume: 177
Pages: 353-365
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] A novel autoantibody against moesin in the serum of patients with MPO-ANCA-associated vasculitis.2013
Author(s)
Suzuki K, Nagao T, Itabashi M, Hamano Y, Sugamata R, Yamazaki Y, Yumura W, Tsukita S, Wang PC, Nakayama T, Suzuki K.
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Journal Title
Nephrol Dial Transplant.
Volume: 2013 Dec 5.
Related Report
Peer Reviewed
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[Journal Article] Rituximab Treatment for PR3-ANCA-Positive Membranoproliferative Glomerulonephritis Associated with Adult-Onset Periodic Fever Syndrome.2012
Author(s)
Hamano Y, Yoshizawa H, Sugase T, Miki T, Ohtani N, Hanawa S, Takeshima E, Morishita Y, Saito O, Takemoto F, Muto S, Yumura W, Kusano, E.
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Journal Title
Case Reports in Nephrol Urol
Volume: 2
Pages: 92-101
Related Report
Peer Reviewed
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