Grant-in-Aid for Scientific Research (C)
The spontaneous crescentic glomerulonephritis-forming/Kinjoh (SCG/Kj) mouse is a unique model of vasculitis with production of MPO-ANCA. Genome wide mapping revealed the non-Fas susceptibility locus of MPO-ANCA on chromosome 1, designated as Man-1. The aim of this study is to identify the responsible gene (s) of Man-1. Interval mapping created single peak of LOD curve on the Gene A, a protein-coding gene with unknown function. Because of the highest LOD score, Gene A was the first positional candidate gene. Recombinant Gene A accelerated the proliferation of murine myeloid cell line Mcl33D. However, transfection of Gene A to Mcl33D did not influenced to expression levels of leukocyte activation markers.We established SCG/Kj-derived interval congenic mice with B6 background. Fortunately, we obtained mice with recombination between B6 and SCG/Kj at the midpoint of Man-1 interval. These mice will help us to narrow down positional candidate genes on Man-1 interval.
Nephrol Dial Transplant.
Clin Exp Immunol
La Presse Medicale & Quarterly Medical Review
Volume: 2013 Dec 5.
Case Reports in Nephrol Urol
Attribution of KAKENHI