| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Research Abstract |
Paraspeckle (PS) is a large RNA-protein complex, consists of several ten RNA binding proteins and NEAT1, a noncoding RNA indispensable for the nuclear body assembly. The current study focuses on the molecular mechanism and nuclear distribution of PS to ellucidate paraspeckles' physiological function.
We established a mouse cell constitutively expressing human NEAT1 RNA so that exogenous 'human PS' can be formed in addition to endogenous 'mouse PS'. In this heterologous system, 'human PS', a chimeric sturacutre of human RNA and mouse proteins was assembled independent of 'mouse PS'. Upon administration of a proteasome inhibitor, both humanPS and mouse PS enlarged independently. Furthermore, we observed that Neat1 loci (endogenous) and NEAT1 loci (exogenous), encoded separate chromosomes relocated vicinity, suggesting PS could be a driving force to recruit attached loci to close proximity.
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