Physiological function of paraspeckles: molecular mechanism of assembly and their nuclear distribution

• Project/Area Number: 23510247
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: System genome science
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: SASAKI Yasunori 独立行政法人産業技術総合研究所, バイオメディカル研究部門主任, 研究員 (30312242)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 核内構造体 / パラスペックル / ノンコーディングRNA / ゲノム / ノンコーディングＲＮＡ

Research Abstract

Paraspeckle (PS) is a large RNA-protein complex, consists of several ten RNA binding proteins and NEAT1, a noncoding RNA indispensable for the nuclear body assembly. The current study focuses on the molecular mechanism and nuclear distribution of PS to ellucidate paraspeckles' physiological function.
We established a mouse cell constitutively expressing human NEAT1 RNA so that exogenous 'human PS' can be formed in addition to endogenous 'mouse PS'. In this heterologous system, 'human PS', a chimeric sturacutre of human RNA and mouse proteins was assembled independent of 'mouse PS'. Upon administration of a proteasome inhibitor, both humanPS and mouse PS enlarged independently. Furthermore, we observed that Neat1 loci (endogenous) and NEAT1 loci (exogenous), encoded separate chromosomes relocated vicinity, suggesting PS could be a driving force to recruit attached loci to close proximity.

Research Products

• [Journal Article] Alternative 3'-end processing of long noncoding RNA initiates construction of nuclear paraspeckles (2012)
  • Author(s): Naganuma T, Nakagawa S, Tanigawa A, Sasaki YF, Goshima N, Hirose T
  • Journal Title: EMBO J Volume: 31巻 Pages: 4020-4034
  • Peer Reviewed
• [Journal Article] Alternative 3'-end processing of long noncoding RNA initiates construction of nuclear paraspeckles (2012)
  • Author(s): Naganuma, T., Nakagawa, S., Tanigawa, A., Sasaki, Y. F., Goshima, N., and Hirose, T
  • Journal Title: EMBO J Volume: 31 Issue: 20 Pages: 4020-4034
  • DOI: 10.1038/emboj.2012.251
  • Peer Reviewed
• [Presentation] ノンコーディング RNA NEAT1 の Xenopus オーソログ (2014)
  • Author(s): 佐々木保典、他9名
  • Organizer: XCIJ 首都圏支部会
  • Place of Presentation: 横浜
• [Presentation] ノンコーディング RNA NEAT1のXenopus オーソログ (2012)
  • Author(s): 佐々木保典、小川朝子、谷川明恵、小沼泰子、伊藤弓弦、廣瀬哲郎
  • Organizer: 日本 RNA 学会
  • Place of Presentation: 仙台
• [Presentation] ノンコーディングRNA NEAT1のXenopusオーソログ (2012)
  • Author(s): 佐々木保典、小川朝子、谷川明恵、小沼泰子、伊藤弓弦、廣瀬哲郎
  • Organizer: 日本RNA学会
  • Place of Presentation: 仙台
• [Presentation] ノンコーディングRNA NEAT1のXenopusオーソログ
  • Author(s): 佐々木保典 他9名
  • Organizer: ＸＣＩＪ首都圏支部会
  • Place of Presentation: 横浜