• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of the elementary processes in the light-driven Cl- pump of halorhodopsin

Research Project

Project/Area Number 23510251
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Living organism molecular science
Research InstitutionHokkaido University

Principal Investigator

KIKUKAWA Takashi  北海道大学, 先端生命科学研究科(研究院), 講師 (20281842)

Co-Investigator(Kenkyū-buntansha) MIYAUCHI Seiji  東邦大学, 薬学部, 教授 (30202352)
Co-Investigator(Renkei-kenkyūsha) TAMOGAMI Jun  松山大学, 薬学部, 助教 (30580089)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsハロロドプシン / フォトサイクル / アニオンポンプ / 膜蛋白質 / レチナール / イオンポンプ
Research Abstract

Halorhodopsin (HR) is an inward light-driven Cl- pump in the membrane of halophilic archaea. Illumination triggers the photoreaction of HR called photocycle where the intermediates of K, L1, L2, N, O, NpHR' form and decay sequentially. During this cyclic reaction, HR releases Cl- to the cytoplasmic (CP) medium and captures another Cl- from the extracellular medium. To examine the elementary processes for Cl- translocation, we analyzed the photocycle using the flash-photolysis technique and obtained the following results: 1) Cl- release and uptake occur during N to O and O to NpHR' transitions, respectively. The subsequent NpHR' to original NpHR transition involves Cl- displacement inside the protein. 2) The Cl- binding affinity decreases at the Cl- releasing step. This decreased binding affinity contributes to maintain the Cl- pumping activity even in the presence of the interior negative membrane potential.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (56 results)

All 2014 2013 2012 2011 Other

All Journal Article (13 results) (of which Peer Reviewed: 13 results) Presentation (40 results) Book (3 results)

  • [Journal Article] Role of Thr218 in the Light-Driven Anion Pump Halorhodopsin from Natronomonas pharaonis2013

    • Author(s)
      Shibasaki, K., Shigemura, H., Kikukawa, T., Kamiya, M., Aizawa, T., Kawano, K., Kamo, N., Demura, M
    • Journal Title

      Biochemistry

      Volume: 52 Issue: 51 Pages: 9257-9268

    • DOI

      10.1021/bi401295e

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Thermodynamic parameters of anion binding to halorhodopsin from Natronomonas pharaonis by isothermal titration calorimetry2013

    • Author(s)
      Hayashi, Saori
    • Journal Title

      Biophysical Chemistry

      Volume: 172 Pages: 61-67

    • DOI

      10.1016/j.bpc.2013.01.001

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Physicochemical Studies of Bacteriorhodopsin Reconstituted in Partially Fluorinated Phosphatidylcholine Bilayers2013

    • Author(s)
      Yoshino, M., Kikukawa, T., Takahashi, H., Takagi, T., Yokoyama, Y., Amii, H., Baba, T., Kanamori, T., Sonoyama, M.
    • Journal Title

      J. Phys. Chem. B

      Volume: 117 Issue: 18 Pages: 5422-5429

    • DOI

      10.1021/jp311665z

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Exporing the active site site structure of a photoreceptor protein by raman optical activity2013

    • Author(s)
      Unno Masashi, kikukawa takashi, Kumauchi Masato, Kamo Naoki
    • Journal Title

      Journal of Physical Chemistry.

      Volume: 117巻 Issue: 5 Pages: 1321-325

    • DOI

      10.1021/jp4001187

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Salt bridge in the conserved His-Asp cluster in Gloeobacter rhodopsin contributes to trimer formation2013

    • Author(s)
      Tsukamoto, T., Kikukawa, T., Kurata, T., Jung, K-H, Kamo, N., Demura, M.
    • Journal Title

      FEBS Lett.

      Volume: 587 Issue: 4 Pages: 322-327

    • DOI

      10.1016/j.febslet.2012.12.022

    • NAID

      120005228320

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Dynamics of Dangling Bonds of Water Molecules in pharaonis Halorhodopsin during Chloride Ion Transportation2012

    • Author(s)
      Furutani, Y., Fujiwara, K., Kimura, T., Kikukawa, T., Demura, M., Kandori, H
    • Journal Title

      J. Phys. Chem. Lett

      Volume: 3 Issue: 20 Pages: 2964-2969

    • DOI

      10.1021/jz301287n

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Homo-trimer Formation and Dissociation of pharaonis Halorhodopsin in Detergent System2012

    • Author(s)
      Tsukamoto, T., Sasaki, T., Fujimoto, K. J., Kikukawa, T., Kamiya, M., Aizawa, T., Kawano, K., Kamo, N., Demura, M
    • Journal Title

      Biophys. J

      Volume: 102 Issue: 12 Pages: 2906-2915

    • DOI

      10.1016/j.bpj.2012.05.008

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Photoinduced proton release in proteorhodopsin at low pH: The possibility of a decrease in the pKa of Asp2272012

    • Author(s)
      Tamogami Jun, Kikukawa Takashi, NaraToshifumi , Shimono Kazumi, Demura Makoto, Kamo Naoki
    • Journal Title

      Biochemistry

      Volume: 51巻 Issue: 46 Pages: 9290-9301

    • DOI

      10.1021/bi300940p

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] "The influence of halide binding on the hydrogen bonding network in the active site of Salinibacter sensory rhodopsin I"2012

    • Author(s)
      L. Reissig, T. Iwata, T. Kikukawa, M. Demura, N. Kamo, H. Kandori and Y. Sudo
    • Journal Title

      Biochemistry

      Volume: 51 Issue: 44 Pages: 8802-8813

    • DOI

      10.1021/bi3009592

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Photo-induced bleaching of sensory rhodopsin II (phoborhodopsin) from Halobacteium salinarum by hydroxylamine2012

    • Author(s)
      Tamogami Jun
    • Journal Title

      Journal of Photochemistry and Photobiology B

      Volume: 106 Pages: 87-94

    • DOI

      10.1016/j.jphotobiol.2011.10.009

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Expression of salinarum halorhodopsin in Escherichia coli cells : Solubilization in the presence of retinal yields the natural state2011

    • Author(s)
      Yamashita Yasutaka
    • Journal Title

      Biochimica et Biophysica Acta-Biomembranes

      Volume: 1808 Issue: 12 Pages: 2905-2912

    • DOI

      10.1016/j.bbamem.2011.08.035

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Photochemistry of acetabularia rhodopsin II from a marine plant, Aceta bularia acetabulum2011

    • Author(s)
      Kikukawa Takashi
    • Journal Title

      Biochemistry

      Volume: 50 Issue: 41 Pages: 8888-8898

    • DOI

      10.1021/bi2009932

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Crystal structure of the Eukaryotic light-driven proton-pumping rhodopsin, Acetabularia rhodopsin II from Marine Algae2011

    • Author(s)
      Wada Takashi
    • Journal Title

      Journal of Molecular Biology

      Volume: 411 Issue: 5 Pages: 986-998

    • DOI

      10.1016/j.jmb.2011.06.028

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 二量体化した Di-O-Tetradecylphosphatidylcholine への膜タンパ ク質バクテリオロドプシンの再構成2014

    • Author(s)
      土田直之、高木俊之、菊川峰志、高橋 浩、金森敏幸、園山正史
    • Organizer
      日本化学会第94春季年会
    • Place of Presentation
      名古屋大学(名古屋市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Analysis of Cl-release and uptake steps of light-driven Cl-pump Natronomonas pharaonis halorhodopsin2013

    • Author(s)
      Kikukawa, T
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      国立京都国際会館(京都市)
    • Year and Date
      2013-10-29
    • Related Report
      2013 Final Research Report
  • [Presentation] Role of Thr218 in light-driven Cl- pump mechanism of Natronomonas pharaonis halorhodopsin2013

    • Author(s)
      Shibasaki, K
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      国立京都国際会館(京都市)
    • Year and Date
      2013-10-29
    • Related Report
      2013 Final Research Report
  • [Presentation] 部分フッ素化リン脂質・非フッ素化リン脂質二成分混合膜に再構成したバクテリオロドプシンの脂質選択性2013

    • Author(s)
      吉野 賢、菊川 峰志、高橋 浩、 高木 俊之、網井 秀樹、金森 敏幸、 園山 正史
    • Organizer
      第13回に本蛋白質科学会年会
    • Place of Presentation
      とりぎん文化会館(鳥取市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 光駆動型 Cl- ポンプハロロドプシンの光反応サイクルにおける荷電性残基の役割2013

    • Author(s)
      大塚 瞭、菊川 峰志、神谷 昌克、 相沢 智康、河野 敬一、加茂 直樹、 出村 誠
    • Organizer
      第13回に本蛋白質科学会年会
    • Place of Presentation
      とりぎん文化会館(鳥取市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ハロロドプシンの光反応サイクルに現れる膜電位依存性2013

    • Author(s)
      日下部 力、菊川 峰志、神谷 昌克、 相沢 智康、河野 敬一、井原 邦夫、 加茂 直樹、出村 誠
    • Organizer
      第13回に本蛋白質科学会年会
    • Place of Presentation
      とりぎん文化会館(鳥取市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Photochemical reaction in Acetabularia rhodopsin I2013

    • Author(s)
      Tamogami, J., Kikukawa, T., Shimono, K., Kimura-Someya, T., Shirouzu, M., Yokoyama, S., Kamo, N.
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      国立京都国際会館(京都市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Analysis of Cl- release and uptake steps of light-driven Cl- pump Natronomonas pharaonis halorhodopsin2013

    • Author(s)
      Kikukawa, T., Kusakabe, C., Kokubo, A., Tsukamoto, T., Kamiya, M., Aizawa, T., Ihara, K., Kamo, N., Demura, M.
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      国立京都国際会館(京都市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Role of Thr218 in light-driven Cl- pump mechanism of Natronomonas pharaonis halorhodopsin2013

    • Author(s)
      Shibasaki, K., Shigemura, H., Kikukawa, T., Kamiya, M., Aizawa, T., Kamo, N., Demura, M.
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      国立京都国際会館(京都市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] A Biophysical Study of Bacteriorhodopsin in Pseudocyclic Phosphatidylcholine Liposome2013

    • Author(s)
      Sonoyama, M., Yoshioka, S., Tsuchida, N., Takagi, T., Takahashi, H., Kikukawa, T., Kanamori, T.
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      国立京都国際会館(京都市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Low affinity of bacteriorhodopsin to partially fluorinated phosphatidylcholine in reconstituted membrane2013

    • Author(s)
      Yoshino, M., Kanayama, K., Kikukawa, T., Takagi, T., Takahashi, H., Yokoyama, Y., Amii, H., Kanamori, T., Somoyama, M.
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      国立京都国際会館(京都市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] リン脂質リポソームに再構成したバクテリオロドプシンの構造・機能に対するアシル鎖部分フッ素化の影響2013

    • Author(s)
      吉野賢、菊川峰志、高橋浩、高木俊之、横山泰範、網井秀樹、金森敏之、園山正史
    • Organizer
      第64回コロイドおよび界面化学討論会
    • Place of Presentation
      名古屋工業大学(名古屋市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Native-like reconstitution of bacteriorhodopsin into novel partially fluorinated phosphatidylcholine lipid bilayer2013

    • Author(s)
      Sonoyama, M., Yoshino, M., Kikukawa, T., Takahashi, H., Takagi, T., Yokoyama, Y., Amii, H., Kanamori, T.
    • Organizer
      The International Conference on Structural Genomics 2013
    • Place of Presentation
      京王プラザホテル(札幌市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 部分フッ素化リン脂質を含む二成分系リン脂質混合膜における 膜タンパク質バクテリオロドプシンの分配挙動2013

    • Author(s)
      吉野賢、金山賢治、高橋浩、高木俊之、菊川峰志、横山泰範、網井秀樹、金森敏之、園山正史
    • Organizer
      第40回生体分子科学討論会
    • Place of Presentation
      大阪大学(吹田市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] When does halorhodopsin capture and release Cl-during photocycle? Examination by spectrum changes of an intrinsic voltage-sensitive dye, bacterioruberin2012

    • Author(s)
      Kikukawa, T
    • Organizer
      15th International Conference on Retinal Proteins
    • Place of Presentation
      Monte Verita Congress Center(Swizarland)
    • Related Report
      2013 Final Research Report
  • [Presentation] When does halorhodopsin capture and release Cl- during photocycle? Examination by spectrum changes of an intrinsic voltage-sensitive dye, bacterioruberin2012

    • Author(s)
      Kikukawa, T., Kokubo, A., Tsukamoto, T., Ihara, K., Kamo, N., Demura, N.
    • Organizer
      15th International Conference on Retinal Proteins
    • Place of Presentation
      Monte Verita Congress Center(Swizarland)
    • Related Report
      2012 Research-status Report
  • [Presentation] The effect of halide binding on the hydrogen bonding network in the active site of Salinibacter sensory rhodopsin I2012

    • Author(s)
      Reissig, L., Iwata, T., Kikukawa, T., Demura, M., Kamo, N., Kandori, H., Sudo, Y.
    • Organizer
      15th International Conference on Retinal Proteins
    • Place of Presentation
      Monte Verita Congress Center(Swizarland)
    • Related Report
      2012 Research-status Report
  • [Presentation] The Proton Release in Proteorhodopsin at Low pH: Decrease in pKa of Asp227 in the excited state2012

    • Author(s)
      Tamogami, J., Kikukawa, T., Nara, T., Shimono, K., Demura, M., Kamo, N.
    • Organizer
      15th International Conference on Retinal Proteins
    • Place of Presentation
      Monte Verita Congress Center(Swizarland)
    • Related Report
      2012 Research-status Report
  • [Presentation] Homo-trimeric structure of Gloeobacter rhodopsin is regulated by the protonation state of Asp121, a counterion of the protonated Schiff base2012

    • Author(s)
      Tsukamoto, T., Kikukawa, T., Kurata, T., Jung, K-H., Kamo, N., Demura, M.
    • Organizer
      15th International Conference on Retinal Proteins
    • Place of Presentation
      Monte Verita Congress Center(Swizarland)
    • Related Report
      2012 Research-status Report
  • [Presentation] Natronomonas pharaonis 由来のセンサリーロドプシンII の光誘起プロトン移動における塩化物イオンの役割2012

    • Author(s)
      Tamogami, J., Iwano, K., Matsuyama, A., Kikukawa, T., Demura, M., Shimono, K., Nara, T., Kamo, N.
    • Organizer
      第50回日本生物物理学会年会
    • Place of Presentation
      名古屋大学(名古屋市)
    • Related Report
      2012 Research-status Report
  • [Presentation] アナべナセンサリーロドプシンの光誘起プロトン移動反応の解析2012

    • Author(s)
      Hasemi, T., Kikukawa, T., Kamiya, M., Aizawa, T., Kawano, K., Jung, K-H., Kamo, N., Demura, M.
    • Organizer
      第50回日本生物物理学会年会
    • Place of Presentation
      名古屋大学(名古屋市)
    • Related Report
      2012 Research-status Report
  • [Presentation] グロエオバクターロドプシンの三量体形成に関する研究2012

    • Author(s)
      Tsukamoto, T., Kikukawa, T., Kamiya, M., Aizawa, T., Kawano, K., Jung, K-H., Kamo, N., Demura, M.
    • Organizer
      第50回日本生物物理学会年会
    • Place of Presentation
      名古屋大学(名古屋市)
    • Related Report
      2012 Research-status Report
  • [Presentation] 時間分解赤外分光法によるファラオニスハロロドプシンの機能発現過程での水の水素結合ダイナミクス2012

    • Author(s)
      Fujiwara, K., Kimura, T., Kikukawa, T., Demura, M., Kandori, H., Furutani, Y.
    • Organizer
      第50回日本生物物理学会年会
    • Place of Presentation
      名古屋大学(名古屋市)
    • Related Report
      2012 Research-status Report
  • [Presentation] 部分フッ素化ホスファチジルコリン小胞に再構成されたバクテリオロドプシンの液晶相における分子集合体2012

    • Author(s)
      Yoshino, M., Kanayama, K., Kikukawa, T., Takagi, T., Takahashi, H., Baba, T., Kanamori, T., Sonoyama, M.
    • Organizer
      第50回日本生物物理学会年会
    • Place of Presentation
      名古屋大学(名古屋市)
    • Related Report
      2012 Research-status Report
  • [Presentation] Laser flash photolysis study on the decay kinetics of the Mphotointermediate of bacteriorhodopsin in diheptanoylphosphocholine micelles2012

    • Author(s)
      Sonoyama, M., Kuwabara, Y., Kikukawa, T.
    • Organizer
      第50回日本生物物理学会年会
    • Place of Presentation
      名古屋大学(名古屋市)
    • Related Report
      2012 Research-status Report
  • [Presentation] 三次元固体NMR 法による膜タンパク質ハロロドプシンの研究2012

    • Author(s)
      田巻初, 江川文子, 神谷昌克, 菊川峰志, 相沢智康, 河野敬一, 藤原敏道, 出村誠
    • Organizer
      第12回日本蛋白質科学会年会
    • Place of Presentation
      名古屋国際会議場(名古屋市)
    • Related Report
      2012 Research-status Report
  • [Presentation] 短鎖リン脂質ジヘプタノイルホスファチジルコリンを用いた可溶化による膜タンパク質バクテリオロドプシンAsp96 のpKa の変化2012

    • Author(s)
      園山正史, 桑原由美子, 菊川峰志
    • Organizer
      第12回日本蛋白質科学会年会
    • Place of Presentation
      名古屋国際会議場(名古屋市)
    • Related Report
      2012 Research-status Report
  • [Presentation] ハロロドプシンの光反応サイクル中に起こるカロテノイドの吸光度変化2012

    • Author(s)
      菊川峰志,小久保麻実, 塚本卓, 井原邦夫, 加茂直樹, 出村誠
    • Organizer
      第12回日本蛋白質科学会年会
    • Place of Presentation
      名古屋国際会議場(名古屋市)
    • Related Report
      2012 Research-status Report
  • [Presentation] APPLICATION OF NOVEL PARTIALLY FLUORINATED PHOSPHOLIPID: RECONSTITUTION OF MEMBRANE PROTEIN BACTERIORHODOPSIN INTO LIPOSOME2012

    • Author(s)
      Sonoyama, M., Yoshino, M., Takagi, T., Kikukawa, T., Takahashi, H., Baba, T., Kanamori, T.
    • Organizer
      20th International symposium on fluorine chemistry
    • Place of Presentation
      京都テルサ(京都市)
    • Related Report
      2012 Research-status Report
  • [Presentation] Volume change during the Cl- transport of Natronomonas pharaonis halorhodopsin2011

    • Author(s)
      Shibasaki, K
    • Organizer
      第49回日本生物物理学会年会
    • Place of Presentation
      兵庫県立大学(姫路市)
    • Year and Date
      2011-09-16
    • Related Report
      2013 Final Research Report
  • [Presentation] 海洋細菌由来のロドプシン様タンパク質プロテオロドプシンの低pH条件下における速いプロトン放出機構

    • Author(s)
      田母神淳,菊川峰志,下野和実,奈良敏文,宗行英朗,加茂直樹
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      北海道大学(札幌市)
    • Related Report
      2011 Research-status Report
  • [Presentation] 非線形サンプリングを用いた膜タンパク質の固体NMR測定

    • Author(s)
      田巻初,江川文子,松木陽,神谷昌克,菊川峰志,相沢智康,河野敬一,藤原敏道,出村誠
    • Organizer
      第11回蛋白質科学会年会
    • Place of Presentation
      ホテル阪急エキスポパーク(大阪府
    • Related Report
      2011 Research-status Report
  • [Presentation] キサントロドプシンの光誘起プロトン移動の解析

    • Author(s)
      菊川峰志,佐藤由美,田母神淳,下野和実,神谷昌克,相沢智康,河野敬一,出村誠,加茂直樹
    • Organizer
      第11回蛋白質科学会年会
    • Place of Presentation
      ホテル阪急エキスポパーク(大阪府)
    • Related Report
      2011 Research-status Report
  • [Presentation] Volume change during the Cl- transport of Natronomonas pharaonis halorhodopsin

    • Author(s)
      柴崎宏介,菊川峰志,神谷昌克,相沢智康,河野敬一,加茂直樹,出村誠
    • Organizer
      第49回日本生物物理学会年会
    • Place of Presentation
      兵庫県立大学(姫路市)
    • Related Report
      2011 Research-status Report
  • [Presentation] The fast proton release of proteorhodopsin at low pH

    • Author(s)
      田母神淳,菊川峰志,下野和実,奈良敏文,宗行英朗,加茂直樹
    • Organizer
      第49回日本生物物理学会年会
    • Place of Presentation
      兵庫県立大学(姫路市)
    • Related Report
      2011 Research-status Report
  • [Presentation] Role of Ser171 for the stabilization of NpHR trimer

    • Author(s)
      塚本卓,寺田浩太,中川摩里恵,田巻初,神谷昌克,菊川峰志,相沢智康,河野敬一,出村 誠
    • Organizer
      第49回日本生物物理学会年会
    • Place of Presentation
      兵庫県立大学(姫路市)
    • Related Report
      2011 Research-status Report
  • [Presentation] Reconstitution of bacteriorhodopsin into partially fluorinated phosphatidylcholine liposomes

    • Author(s)
      吉野賢,菊川峰志,横山泰範,高木俊之,高橋浩,出村誠,馬場照彦,金森敏幸,園山正史
    • Organizer
      第49回日本生物物理学会年会
    • Place of Presentation
      兵庫県立大学(姫路市)
    • Related Report
      2011 Research-status Report
  • [Presentation] Decay kinetics of the M photointermediate of bacteriorhodopsin in short chain phosphatidylcholine micelles

    • Author(s)
      桑原由美子,福元美奈,菊川峰志,出村誠,園山正史
    • Organizer
      第49回日本生物物理学会年会
    • Place of Presentation
      兵庫県立大学(姫路市)
    • Related Report
      2011 Research-status Report
  • [Presentation] 短鎖リン脂質ジヘプタノイルホスファチジルコリンミセル中の膜タンパク質バクテリオロドプシンの不均一性

    • Author(s)
      桑原由美子,菊川峰志,出村誠,園山正史
    • Organizer
      日本化学会第92春季年会
    • Place of Presentation
      慶應義塾大学(横浜市)
    • Related Report
      2011 Research-status Report
  • [Presentation] STRUCTURE AND FUNCTION OF BACTERIORHODOPSIN RECONSTITUTED INTO PARTIALLY FLUORINATED PHOSPHATIDYLCHOLINE LIPOSOME

    • Author(s)
      Yoshino, M, Kikukawa, T., Takagi, T., Yokoyama, Y., Takahashi, H., Baba, T., Kanamori, T., Demura, M., Sonoyama, M.
    • Organizer
      「揺らぎと生体機能」第5回公開シンポジウム
    • Place of Presentation
      東大寺総合文化センター(奈良市)
    • Related Report
      2011 Research-status Report
  • [Book] -光工学と遺伝学による行動制御技術の最前線-2013

    • Author(s)
      出村誠, 菊川峰志, 加茂直樹
    • Publisher
      エヌ・ティー・エス, オプトジェネティクス
    • Related Report
      2013 Final Research Report
  • [Book] オプトジェネティクス-光工学と遺伝学による行動制御技術の最前線-2013

    • Author(s)
      出村誠, 菊川峰志, 加茂直樹
    • Publisher
      エヌ・ティー・エス
    • Related Report
      2012 Research-status Report
  • [Book] Photo-induced Proton Transfers of Microbial Rhodopsins, In Molecular Photochemistry - Various Aspects (Dr. Satyen Saha, Ed.)2012

    • Author(s)
      Kikukawa, T., Tamogami, J., Shimono, K., Demura, M., Nara, T., Kamo, N.
    • Publisher
      InTech
    • Related Report
      2011 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi