| Project/Area Number |
23510253
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Saitama University |
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Principal Investigator |
NEMOTO Naoto 埼玉大学, 理工学研究科, 准教授 (60509727)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 進化分子工学 / ペプチド / アプタマー / アミノ基認識 / 分子間相互作用 / 試験管内淘汰 / ｃDNA display / mRNA display / ペプチドアプタマー / 分子認識 / 架橋構造 / ジスルフィド結合 / 進化工学 / In vitro selection / cDNA display / Peptide aptamer / mRNA/cDNA display / 抗体低分子化 / 低分子認識分子 / 分子間相互作用解析 / 表面プラズモン共鳴法 / プルダウン法 |
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| Research Abstract |
We have succeeded to improve the preparation of cDNA display molecule easily and efficiently with a one-pot preparational manner. Furthermore, we demonstrated the biotin binding capacity of streptavidin magnetic beads decreased by mRNA-binding ribosomes, resulted in the low yield of cDNA display molecules. Secondly, we developed an easy and rapid molecular-protein interaction analysis using a cell-free translation and a novel puromycin-linker by a pull-down manner. Finally, by using these techniques, in vitro selection using 30 amino acids random library containing cysteine against amino group on the solid-phase was performed. As a result, an amino group-binding peptide aptamer containing two disulfide bonds which have a unique structure has explored. This shows that a peptide has capability to recognize small molecules with a low-molecular weight. In the future, peptides will be more and more applicable for nanotechnology as a recogniton material of nano sturcture.
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