System-level analysis of cell signaling perturbation by high-resolution mass spectrometry
| Project/Area Number |
23510254
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
OYAMA Masaaki 東京大学, 医科学研究所, 准教授 (30422398)
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| Co-Investigator(Kenkyū-buntansha) |
HATA Hiroko 東京大学, 医科学研究所, 技術専門職員 (80401256)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | プロテオミクス / シグナル伝達 / システムバイオロジー / リン酸化 / 定量生物学 / 国際情報交換 / 国際情報交流 |
|---|
| Research Abstract |
The signal transduction system regulates fundamental biological events such as cell growth and differentiation. Dysregulation of this machinery is known to be closely associated with various types of diseases including cancer. In this research project, we performed a system-level analysis of signaling network perturbation in H. pylori-infected gastric epithelial cells using high resolution nanoLC-MS/MS system coupled with SILAC technology. Here, we report time-resolved description of the phosphoproteome dynamics upon H. pylori infection as well as the interactome of the major virulence factor, CagA.
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Report
(4 results)
Research Products
(50 results)
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[Journal Article] Newly identified minor phosphorylation site threonine-279 of measles virus nucleoprotein is a prerequisite for nucleocapsid formation.2014
Author(s)
Sugai, A., Sato, H., Hagiwara, K., Kozuka-Hata, H., Oyama, M., Yoneda, M., Kai, C.
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Journal Title
J Virol
Volume: 88(2)
Pages: 1140-1149
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Proteomic analysis of the royal jelly and characterization of the functions of its derivation glands in the honeybee2013
Author(s)
Fujita, T., Kozuka-Hata, H., Ao-Kondo, H., Kunieda, T., Oyama, M. and Kubo, T.
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Journal Title
J Proteome Res
Volume: 12
Pages: 404-411
DOI
Related Report
Peer Reviewed
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[Journal Article] The cancer stem cell marker CD133 interacts with plakoglobin and controls desmoglein-2 protein levels.2013
Author(s)
Koyama-Nasu R, Takahashi R, Yanagida S, Nasu-Nishimura Y, Oyama M, Kozuka-Hata H, Haruta R, Manabe E, Hoshino-Okubo A, Omi H, Yanaihara N, Okamoto A, Tanaka T, and Akiyama T.
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Journal Title
DOI
Related Report
Peer Reviewed
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[Journal Article] F1Fo-ATPase, F-type proton-translocating ATPase, at the plasma membrane is critical for efficient influenza virus budding2012
Author(s)
Gorai T, Goto H, Noda T, Watanabe T, Kozuka-Hata H, Oyama M, Takano R, Neumann G, Watanabe S, Kawaoka Y
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Journal Title
Proc Natl Acad Sci USA
Volume: 109(12)
Pages: 4615-20
DOI
Related Report
Peer Reviewed
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[Journal Article] Identification of three new autoantibodies associated with systemic lupus erythematosus using two proteomic approaches.2011
Author(s)
Katsumata Y, Kawaguchi Y, Baba S, Hattori S, Tahara K, Ito K, Iwasaki T, Yamaguchi N, Oyama M, Kozuka-Hata H, Hattori H, Nagata K, Yamanaka H, and Hara M.
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Journal Title
Mol Cell Proteomics
Volume: 10
DOI
Related Report
Peer Reviewed
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