Development of the technology for bone tissue regeneration using NELL1 protein
Project/Area Number |
23510255
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Living organism molecular science
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Research Institution | Nagoya University |
Principal Investigator |
NIIMI Tomoaki 名古屋大学, 生命農学研究科, 助教 (30377791)
|
Co-Investigator(Renkei-kenkyūsha) |
KURODA Shun'ichi 大阪大学, 産業科学研究所, 教授 (60263406)
|
Project Period (FY) |
2011-04-28 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
|
Keywords | 骨分化 / 細胞接着 / 再生医療 / 骨芽細胞 |
Outline of Final Research Achievements |
The NELL1 gene was originally identified in craniosynostosis patients as being specifically upregulated within prematurely fusing sutures. Because of its potent osteoinductive activity, NELL1 protein may be useful for bone regeneration therapy. However, there is little knowledge regarding NELL1 receptors and NELL1-mediated signaling pathways. In this study, we demonstrated that NELL1 promoted osteoblastic cell adhesion through cell-binding sites localized to the C-terminal region of NELL1. We also identified integrin β1 as the major cellular receptor necessary for cell adhesion onto NELL1 and the focal adhesion kinase (FAK)-mitogen-activated protein kinase (MAPK) pathway is involved in NELL1 signaling.
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Report
(5 results)
Research Products
(11 results)
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[Journal Article] Oligomerization-induced conformational change in the C-terminal region of Nel-like molecule 1 (NELL1) protein is necessary for the efficient mediation of murine MC3T3-E1 cell adhesion and spreading2014
Author(s)
Nakamura, Y., Hasebe, A., Takahashi, K., Iijima, M., Yoshimoto, N., Maturana, A. D., Ting, K., Kuroda, S., and Niimi T.
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Journal Title
Journal of Biological Chemistry
Volume: 289
Issue: 14
Pages: 9781-9794
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] The C-terminal region of NELL1 mediates osteoblastic cell adhesion through integrin α3β1.2012
Author(s)
Hasebe, A., Nakamura, Y., Tashima, H., Takahashi, K., Iijima, M., Yoshimoto, N., Ting, K., Kuroda, S., and Niimi, T.
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Journal Title
FEBS Letters
Volume: 586
Issue: 16
Pages: 2500-2506
DOI
Related Report
Peer Reviewed
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[Journal Article] NELL-1 promotes cell adhesion and differentiation via Integrin β1.2012
Author(s)
Shen, J., James, A. W., Chung, J., Lee, K., Zhang, J. B., Ho, S., Lee, K. S., Kim, T. M., Niimi, T., Kuroda, S., Ting, K., and Soo, C.
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Journal Title
Journal of Cellular Biochemistry
Volume: 113
Issue: 12
Pages: 3620-3628
DOI
Related Report
Peer Reviewed
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[Presentation] Characterization of bone-inducing protein NELL1 produced by HEK293-F cells.2012
Author(s)
Hasebe, A., Mayahara, M., Narita, K., Oda, M., Nakamura, M., Kuroda, S. and Niimi, T.
Organizer
The 25th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2012)
Place of Presentation
名古屋国際会議場
Related Report
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