| Budget Amount *help |
¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Research Abstract |
The ALA-induced accumulation of protoporphyrin and photodynamic therapy (PDT) have been used for the diagnosis of cancer. Several factors contribute to the high tumor specificity of ALA-induced accumulation of protoporphyrin as a detection marker for the photodiagnosis of tumors, and we gave evidences that protoporphyrin accumulates owing to the limited capacity for ferrochelatase reaction. Ferrochelatase consists of an iron-sulfur cluster and its expression in tumor cells was decreased due to the low expression of mitochondrial iron-chaperon frataxin unregulated by p53. Thus, mitochondrial dysfunctions, decreased utilization of iron in mitochondria and the recycling of heme-iron can be crucial factors for ALA-PDT. Additional factors including mitoferrin, siderflexin and ABCG2 are also involved in the accumulation of protoporphyrin. Tumor selectivity of ALA-PDT is due to the preferential uptake of ALA through neurotransmitter transporters from the blood stream.
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