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A convenient proteomic system for kinase substrate identification

Research Project

Project/Area Number 23510261
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Living organism molecular science
Research InstitutionKitasato University

Principal Investigator

HATTORI Seisuke  北里大学, 薬学部, 教授 (50143508)

Co-Investigator(Kenkyū-buntansha) SATO Tatsuhiro  北里大学, 薬学部, 助教 (70547893)
Co-Investigator(Renkei-kenkyūsha) IIDA Naoyuki  北里大学, 薬学部, 講師 (10376618)
SHIBAGAKI Yoshio  北里大学, 薬学部, 講師 (90235565)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsプロテオミクス / キナーゼ / 二次元ゲル電気泳動 / リン酸化 / ERK / mTOR / p38 MAPキナーゼ
Research Abstract

We established an in vitro dephosphorylation-phosphorylation system, by which we identified 7 novel p38 MAP kinase substrates. We also identified novel ERK and mTOR substrates as kinase-bound proteins. ERK phosphorylation of adducin attenuated adducin-actin filament interaction. Furthermore, we identified Filamin A as a novel mTOR substrate. mTOR phosphorylation of Filaimin A controls focal adhesions.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (43 results)

All 2014 2013 2012 2011 Other

All Journal Article (12 results) (of which Peer Reviewed: 8 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (27 results) Book (2 results) Remarks (2 results)

  • [Journal Article] Proteomic identification of p38 MAP kinase substrates using in vitro phosphorylation2014

    • Author(s)
      Iida N, Fujita M, Miyazawa K, Kobayashi M, Hattori S
    • Journal Title

      Electrophoresis

      Volume: 35 Issue: 4 Pages: 554-562

    • DOI

      10.1002/elps.201300392

    • NAID

      130006999187

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Journal Article] An efficient screening system for influenza virus cap-dependent endonuclease inhibitors2014

    • Author(s)
      Shibagaki Y, Ikuta N, Iguchi S, Takaki K, Watanabe S, Kaihotsu M, Masuda C, Maeyama K, Mizumoto K, Hattori S
    • Journal Title

      J Virol Methods

      Volume: S0166-0934(14) Pages: 48-2

    • DOI

      10.1016/j.jviromet.2014.02.005

    • Related Report
      2013 Final Research Report
  • [Journal Article] An adaptor protein complex of FRS2beta and CIN85/CD2AP provides a novel mechanism for ErbB2/HER2 protein downregulation2013

    • Author(s)
      後藤 典子
    • Journal Title

      Cancer Sci

      Volume: 104 Issue: 3 Pages: 345-352

    • DOI

      10.1111/cas.12086

    • Related Report
      2013 Final Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] proteomic approach for the elucidation of stimulus- and substrate-specificity of ectodomain shedding.2013

    • Author(s)
      Shirakabe K., Shibagaki Y., Yoshimura A., Koyasu S., Hattori S.
    • Journal Title

      J Proteomics

      Volume: 98 Pages: 233-243

    • DOI

      10.1016/j.jprot.2014.01.012

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] SIRPα interacts with nephrin at the podocyte slit diaphragm.2012

    • Author(s)
      Kajiho Y, Harita Y, Kurihara H, Horita S, Matsunaga A, Tsurumi H, Kanda S, Sugawara N, Miura K, Sekine T, Hattori S, Hattori M, Igarashi T.
    • Journal Title

      FEBS J.

      Volume: 279 Issue: 17 Pages: 3010-3021

    • DOI

      10.1111/j.1742-4658.2012.08682.x

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] VIP36 protein is a target of ectodomain shedding and regulates phagocytosis in macrophage Raw 264.7 cells.2011

    • Author(s)
      Shirakabe K, Hattori S, Seiki M. Koyasu S, Okada Y.
    • Journal Title

      J Biol Chem.

      Volume: 16;286(50) Issue: 50 Pages: 43154-63

    • DOI

      10.1074/jbc.m111.275586

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Tyrosine phosphorylation-dependent activation of TRPC6 regulated by PLC-γ1 and nephrin: effect of mutations associated with focal segmental glomerulosclerosis2011

    • Author(s)
      Kanda S, Harita Y, Shibagaki Y, Sekine T, Igarashi T, Inoue T, Hattori S
    • Journal Title

      Mol Biol Cell

      Volume: 22 Issue: 11 Pages: 1824-1835

    • DOI

      10.1091/mbc.e10-12-0929

    • Related Report
      2013 Final Research Report
  • [Journal Article] VIP36 protein is a target of ectodomain shedding and regulates phagocytosis in macrophage Raw 264.7 cells.2011

    • Author(s)
      Shirakabe K, Hattori S, Seiki M, Koyasu S, Okada Y
    • Journal Title

      J Biol Chem

      Volume: 286 Pages: 43154-43163

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Tyrosine phosphorylation-dependent activation of TRPC6 regulated by PLC-γ1 and nephrin: effect of mutations associated with focal segmental glomerulosclerosis.2011

    • Author(s)
      Mol Biol Cell.
    • Journal Title

      Kanda S, Harita Y, Shibagaki Y, Sekine T, Igarashi T, Inoue T, Hattori S.

      Volume: 22 Pages: 1824-1835

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Identification of three new autoantibodies associated with systemic lupus erythematosus using two proteomic approaches.2011

    • Author(s)
      Katsumata Y, Kawaguchi Y, Baba S, Hattori S, Tahara K, Ito K, Iwasaki T, Yamaguchi N, Oyama M, Kozuka-Hata H, Hattori H, Nagata K, Yamanaka H, Hara M.
    • Journal Title

      Mol Cell Proteomics.

      Volume: 10

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Tyrosine kinase signaling in kidney glomerular podocytes.2011

    • Author(s)
      Kanda S, Harita Y, Hattori S.
    • Journal Title

      J Signal Transduct

      Volume: 05 Pages: 317852-317852

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] A novel CD3-specific antibody induces immunosuppression via impaired phosphorylation of LAT and PLC γ 1 following T-cell stimulation

    • Author(s)
      Shiheido H, Aoyama T, Takahashi H, Hanaoka K, Abe T, Nishida E, Chen C, Koga O, Hikida M, Shibagaki Y, Morita A, Nikawa T, Hattori S, Watanabe T, Shimizu J
    • Journal Title

      Eur J Immunol

      Volume: in press

    • Related Report
      2013 Final Research Report
  • [Presentation] mTORC2はfilamin Aを介して接着斑形成と細胞運動を制御する2013

    • Author(s)
      佐藤龍洋,石井淳子,太田悠貴,佐々木恵理,柴垣芳夫,服部成介
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド、神戸
    • Year and Date
      2013-12-05
    • Related Report
      2013 Final Research Report
  • [Presentation] EPLIN (Epithelialprotein lost in neoplasm)は接着斑を安定化し、PDGF刺激によるメサンギウム細胞の運動性を制御する2013

    • Author(s)
      鶴見晴子,張田豊,栗原秀剛,林健二,小迫英尊,服部元史,服部成介,岡明,五十嵐隆
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド、神戸
    • Year and Date
      2013-12-05
    • Related Report
      2013 Final Research Report
  • [Presentation] ERKによる adducinのリン酸化は、細胞の突起伸長や細胞間接着を調節する2013

    • Author(s)
      吉本真吾,小川瑞基,鳥井鈴子,根岸豊,飯田直幸,服部成介
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド、神戸
    • Year and Date
      2013-12-04
    • Related Report
      2013 Final Research Report
  • [Presentation] プロテオミクス解析によるシェディングの刺激・基質特異性の解明2013

    • Author(s)
      白壁恭子,柴垣芳夫,吉村昭彦,小安重夫,服部成介
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド、神戸
    • Year and Date
      2013-12-03
    • Related Report
      2013 Final Research Report
  • [Presentation] Identification of Novel p38 MAP Kinase Substrates Using In Vitro Phosphorylation2013

    • Author(s)
      Naoyuki Iida, Masayuki Fujita, Kohtaro Miyazawa, Michimoto Kobayashi, Seisuke Hattori
    • Organizer
      HUPO 12th annual world congress
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2013-09-17
    • Related Report
      2013 Final Research Report
  • [Presentation] mTOR Complex 2 Phosphorylates Filamin A to Regulate Cell Migration2013

    • Author(s)
      Tatsuhiro Sato, Yoshio Shibagaki, Seisuke Hattori
    • Organizer
      HUPO 12th annual world congress
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2013-09-17
    • Related Report
      2013 Final Research Report
  • [Presentation] ERKは adducinをリン酸化し、 F-actinとの結合を調節する2012

    • Author(s)
      飯田直幸 ,吉本真吾 ,根岸豊 ,吉村憲彦 ,柴垣芳夫 ,服部成介
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡マリンメッセ、福岡
    • Year and Date
      2012-12-14
    • Related Report
      2013 Final Research Report
  • [Presentation] 低分子量G蛋白質Rhebによるヌクレオチド生合成への関与2012

    • Author(s)
      赤須仁美 ,佐藤龍洋 ,柴垣芳夫 ,服部成介
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡マリンメッセ、福岡
    • Year and Date
      2012-12-12
    • Related Report
      2013 Final Research Report
  • [Presentation] mTORC2は Filamin Aを介して細胞運動を制御する2012

    • Author(s)
      佐藤龍洋 ,柴垣芳夫 ,服部成介
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡マリンメッセ、福岡
    • Year and Date
      2012-12-12
    • Related Report
      2013 Final Research Report
  • [Presentation] 神経分化におけるmTORの機能解析2012

    • Author(s)
      本屋勇樹 ,佐藤龍洋 ,服部成介
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡マリンメッセ、福岡
    • Year and Date
      2012-12-12
    • Related Report
      2013 Final Research Report
  • [Presentation] VIP36 is a target of ectodomain shedding and regulates phagocytosis in macrophage.2011

    • Author(s)
      Shirakabe, K., Hattori, S., Seiki, M., Koyasu, S., Okada, Y.
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2011 Research-status Report
  • [Presentation] 低分子量Gタンパク質Rhebの新規シグナル伝達の解析2011

    • Author(s)
      赤須仁美、佐藤龍洋、柴垣芳夫、服部成介
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2011 Research-status Report
  • [Presentation] 持地保徳、柴垣芳夫、服部成介、太田安隆2011

    • Author(s)
      キネシンファミリータンパク質KIF11はFilGAP結合タンパク質である
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2011 Research-status Report
  • [Presentation] 飯田直幸、吉村憲彦、村越水香、武田彩香、高橋沙弥加、種五敦史、柴垣芳夫、服部成介2011

    • Author(s)
      Proteomic identification of novel ERK substrates using in vitro phosphorylation: ERK phosphorylates adducin and regulates its interaction with F-actin.
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2011 Research-status Report
  • [Presentation] Ishido, N., Nagai, H., Kobayashi, H., Sako, Y., Arai, T., Hattori, S., Matsuda, M., Fukuda, M., Nakamura, T.2011

    • Author(s)
      Analysis of Rab35 function in neurite outgrowth using FRET biosensors.
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2011 Research-status Report
  • [Presentation] 柴垣芳夫、生田尚子、井口幸子、水本清久、服部成介2011

    • Author(s)
      抗インフルエンザウイルス創薬のためのCapped RNAの合成法の開発.
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2011 Research-status Report
  • [Presentation] Identification of novel p38 MAP kinase substrates using in vitro phosphorylation

    • Author(s)
      Iida N, Fujita M, Miyazawa K, Kobayashi M, Hattori S
    • Organizer
      HUPO 12th annual world congress
    • Place of Presentation
      Yokohama
    • Related Report
      2013 Annual Research Report
  • [Presentation] mTOR complex 2 phosphorylates Filamin A to regulate cell migration.

    • Author(s)
      Sato T, Shibagaki Y, Hattori S
    • Organizer
      HUPO 12th annual world congress
    • Place of Presentation
      Yokohama
    • Related Report
      2013 Annual Research Report
  • [Presentation] ERKによるadducinのリン酸化は、細胞の突起伸長や細胞間接着を調節する

    • Author(s)
      吉本 真吾,小川 瑞基,鳥井 鈴子,根岸 豊,飯田 直幸,服部 成介
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
  • [Presentation] プロテオミクス解析によるシェディングの刺激・基質特異性の解明

    • Author(s)
      白壁 恭子,柴垣 芳夫,吉村 昭彦,小安 重夫,服部 成介
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
  • [Presentation] mTORC2 はfilamin A を介して接着斑形成と細胞運動を制御する

    • Author(s)
      佐藤 龍洋,石井 淳子,太田 悠貴,佐々木 恵理,柴垣 芳夫,服部 成介
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
  • [Presentation] ERKはadducinをリン酸化し、F-actinとの結合を調節する

    • Author(s)
      飯田 直幸, 吉本 真吾, 根岸 豊, 吉村 憲彦, 柴垣 芳夫, 服部 成介
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] RacGAP因子FilGAPはAfadinと結合し、腎糸球体上皮細胞におけるアクチン細胞骨格形成を制御する

    • Author(s)
      椎野 達大, 斉藤 康二, 望月 優加, 栗原 秀剛2, 張田 豊, 柴垣 芳夫, 服部 成介, 太田 安隆
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] FilGAPとAGAP1の相互作用によるRac1シグナルとArf1シグナルのクロストーク

    • Author(s)
      中村 遥, 柴垣 芳夫, 服部 成介, 太田 安隆
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] 低分子量G蛋白質Rhebによるヌクレオチド生合成への関与

    • Author(s)
      赤須 仁美, 佐藤 龍洋, 柴垣 芳夫, 服部 成介
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] mTORC2はFilamin Aを介して細胞運動を制御する

    • Author(s)
      佐藤 龍洋, 柴垣 芳夫, 服部 成介
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] 神経分化におけるmTORの機能解析

    • Author(s)
      本屋 勇樹, 佐藤 龍洋, 服部 成介
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report
  • [Book] 新細胞生物学2013

    • Author(s)
      服部成介、竹鼻眞、高橋悟、野尻久雄他
    • Total Pages
      265
    • Publisher
      廣川書店
    • Related Report
      2012 Research-status Report
  • [Book] よくわかるゲノム医学2011

    • Author(s)
      服部成介、菅野-水島純子、菅野純夫
    • Total Pages
      203
    • Publisher
      羊土社
    • Related Report
      2011 Research-status Report
  • [Remarks] ホームページ

    • URL

      http://www.pharm.kitasato-u.ac.jp/biochemistry/

    • Related Report
      2013 Final Research Report
  • [Remarks] 北里大学薬学部生化学教室

    • URL

      http://www.pharm.kitasato-u.ac.jp/biochemistry/

    • Related Report
      2013 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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