| Project/Area Number |
23510261
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SATO Tatsuhiro 北里大学, 薬学部, 助教 (70547893)
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| Co-Investigator(Renkei-kenkyūsha) |
IIDA Naoyuki 北里大学, 薬学部, 講師 (10376618)
SHIBAGAKI Yoshio 北里大学, 薬学部, 講師 (90235565)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | プロテオミクス / キナーゼ / 二次元ゲル電気泳動 / リン酸化 / ERK / mTOR / p38 MAPキナーゼ |
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| Research Abstract |
We established an in vitro dephosphorylation-phosphorylation system, by which we identified 7 novel p38 MAP kinase substrates. We also identified novel ERK and mTOR substrates as kinase-bound proteins. ERK phosphorylation of adducin attenuated adducin-actin filament interaction. Furthermore, we identified Filamin A as a novel mTOR substrate. mTOR phosphorylation of Filaimin A controls focal adhesions.
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