Studies on the molecular mechanism of 5-fluorouracil response proteins using a proteomic approach
Project/Area Number |
23510267
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Living organism molecular science
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Research Institution | Osaka Medical College |
Principal Investigator |
SAKAI Akiko 大阪医科大学, 医学部, 講師 (30225750)
|
Co-Investigator(Kenkyū-buntansha) |
TANAKA Satoru 大阪医科大学, 医学部, 助教 (50595741)
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Project Period (FY) |
2011-04-28 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 抗癌剤耐性 / プロテオミクス / HSPB1 / オリゴマー化 / サイトケラチン / リン酸化 / 耐性獲得マーカー / 翻訳後修飾 / オリゴマー構造 |
Outline of Final Research Achievements |
The heat-shock protein (HSP) B1 expression increased with the acquisition of 5-fluorouracil resistance. We analyzed the phosphorylation and oligomeric structure of HSPB1 by one- and two-dimensional western blotting via isoelectric focusing and blue native polyacrylamide gel electrophoresis. The oligomeric structures of HSPB1 in resistant cells differed in molecular mass from those in sensitive cells, suggesting that drug resistance alters the phosphorylation and dissociability of HSPB1 oligomers. Further, cytokeratin 8, 18, 19 were identified the proteins that interact with HSPB1 by co-immunoprecipitation.
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Report
(5 results)
Research Products
(15 results)
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[Journal Article] Phase II Study of Neoadjuvant Anthracycline-Based Regimens Combined With Nanoparticle Albumin-Bound Paclitaxel and Trastuzumab for Human Epidermal Growth Factor Receptor 2-Positive Operable Breast Cancer.2015
Author(s)
Tanaka S, Iwamoto M, Kimura K, Matsunami N, Morishima H, Yoshidome K, Nomura T, Morimoto T, Yamamoto D, Tsubota Y, Kobayashi T, Uchiyama K.
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Journal Title
Clin Breast Cancer
Volume: 15
Issue: 3
Pages: 191-196
DOI
Related Report
Peer Reviewed / Open Access
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