Functional analysis of isoflavonoid biosynthesis related transcription factors using gene co-expression network analysis
| Project/Area Number |
23510272
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Kazusa DNA Research Institute |
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Principal Investigator |
SUZUKI Hideyuki 公益財団法人かずさＤＮＡ研究所, 産業基盤開発研究部, 主席研究員 (80276162)
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| Co-Investigator(Renkei-kenkyūsha) |
OGATA Yoshiyuki 大阪府立大学, 大学院生命環境科学研究科, 准教授 (90446542)
AKASHI Tomoyoshi 日本大学, 生物資源科学部, 講師 (80328707)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 遺伝子共発現ネットワーク解析 / EST解析 / DNAマイクロアレイ / 次世代シークエンサー / 転写因子 / アヤメ科植物 / イソフラボノイド生合成 / 不定根培養 / 遺伝子共発現ネットワーク / DNA マイクロアレイ |
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| Research Abstract |
In order to isolate transcription factors involved in isoflavonoid biosynthesis, we established an EST (expressed sequence tag) data in Iris germanica root cultures, accumulating isoflavonoid, using next-generation sequencing instrument illumina HiSeq 1000 with 100 bp paired-end reads. Furthermore, we carrying out transcript expression analysis (using a custom Iris DNA array) in subculturing time-course experiment of Iris germanica root cultured cells for gene discovery in gene co-expression network analysis.
We established standalone Confeito Java-GUI (gene co-expression network analysis) software that extracted highly-interconnected gene modules. Using the public Arabidopsis DNA array resource data (more than 9 thousands), we have shown an application of our developed Confeito Java-GUI software to isolate gene co-expression network module in Arabidopsis aliphatic glucosinolate biosynthesis.
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Report
(4 results)
Research Products
(24 results)
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[Presentation] Metabolomics-oriented molecular cloning and functional characterization of flavonoid 8-hydroxylase from Lotus japonicas2012
Author(s)
Kota Kera, Norimoto Shimada, Tomoyoshi Akashi, Toshio Aoki, Tina Kanamori, Daisaku Ohta, Koh Aoki, Daisuke Shibata, Hideyuki Suzuki
Organizer
International Conference of Natural Products Biosynthesis(ICNPB)
Place of Presentation
Awaji Yumebutai, Awaji, Japan
Year and Date
2012-06-19
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[Book] Multi-Gene Transformation for Pathway Engineering of Secondary Metabolites. The Handbook of Plant Metabolomics. Metabolite Profiling and Networking, Chapter No122013
Author(s)
Suzuki H, Takita E, Ohyama K, Sawai S, Seki H, Sakurai N, Muranaka T, Ishimoto M, Sudo H, Saito K, Shibata D(W Weckwerth, Kahl G eds)
Related Report
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[Book] The Handbook of Plant Metabolomics. Metabolite Profiling and Networking2013
Author(s)
Suzuki H, Takita E, Ohyama K, Sawai S, Seki H, Sakurai N, Muranaka T, Ishimoto M, Sudo H, Saito K, Shibata D
Total Pages
18
Publisher
Wiley-VCH Verlag GmbH & Co.
Related Report
