| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
Development of the coenzyme regeneration system for reduction of azo dyes by azoreductase on the electrode, NAD modificated by ferrocene moiety was synthesized and was active for four other NAD needed enzymes without inhibitions. Unfortunately, obtained ferrocene modified NAD was indicated low electrode activity. It was found that the coenzyme regeneration system for reduction of azo dyes by azoreductase with coupling to another reductase were promising for new biosensor of dramatic color change.
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