| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Research Abstract |
We developed a binuclear Terbium(III) complexe which selectively responded to phosphotyrosine (pTyr) and emitted luminescence. With the use of the complex, phosphorylation of tyrosine and dephosphorylation of phosphotyrosine in peptides by protein tyrosine kinase(PTK) and by protein tyrosine phosphatase(PTP), respectively, were monitored in real-time. Furthermore, highly complicated systems, where a PTK and a PTP coexisted together, were analyzed by the present system. A straightforward and visual method to assess inhibitors on these enzymes was also developed.
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