Imaging the activation behavior of ER stress sensors
| Project/Area Number |
23510283
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Chemical biology
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| Research Institution | Keio University |
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Principal Investigator |
TASHIRO ETSU 慶應義塾大学, 理工学部, 講師 (00365446)
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| Co-Investigator(Renkei-kenkyūsha) |
IMOTO Masaya 慶應義塾大学, 理工学部, 教授 (60213253)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | 小胞体ストレス / イメージング / センサータンパク質 / UPR / 比較解析 / PERK / IRE1α / BiFC / 小分子化合物 / ATF6 / IRE1 |
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| Research Abstract |
In this study, we compared the expression patterns of nine UPR target genes induced by seven UPR-inducing compounds with different modes of action. As a result, it was suggested that the existence of at least three types of ER stress sensors' activation profiles and UPR target gene expression profiles, which depend on the mode of actions of the compounds.
Moreover, we developed a new detection system for the activation of IRE1a by evaluating dimerization of it by bimolecular fluorescent complementation (BiFC) assay. By detecting the fluorescence derived from the reconstituted cerulean, this assay system enabled us to distinguish the activation behaviors of IRE1a as to ER stress-inducing compounds.
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Report
(4 results)
Research Products
(54 results)
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[Journal Article] Boronic Acid Inhibitors of Acyl Protein Thioesterase 1 and 22013
Author(s)
Zimmermann TJ, Burger M, Tashiro E, Kondoh Y, Martinez NE, Gormer K, Rosin-Steiner S, Shimizu T, Ozaki S, Mikoshiba K, Watanabe N, Hall D, Vetter IR, Osada H, Hedberg C, Waldmann H
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Journal Title
Chem Bio Chem
Volume: 14
Pages: 115-22
Related Report
Peer Reviewed
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[Journal Article] Identification of Toyocamycin, an agent cytotoxic for multiple myeloma cells, as a potent inhibitor of ER stress-induced XBP1 mRNA splicing2012
Author(s)
Ri M, Tashiro E, Oikawa D, Shinjo S, Tokuda M, Yokouchi Y, Narita T, Masaki A, Ito A, Ding J, Kusumoto S, Ishida T, Komatsu H, Shiotsu Y, Ueda R, Iwawaki T, Imoto M, Iida S
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Journal Title
Blood Cancer J.
Volume: e79
Related Report
Peer Reviewed
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[Journal Article] Antitumor effects of novel highly hydrophilic and non-ATP-competitive MEK1/2 inhibitor, SMK-172012
Author(s)
Kiga M, Tanzawa F, Iwasaki S, Inaba F, Fujiwara K, Iwadare H, Echigo T, Nakamura Y, Shibata T, Suzuki K, Yasumatsu, I Nakayama A, Sasazawa Y, Tashiro E, Imoto M , S. Kurakata S
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Journal Title
Anticancer Drugs
Volume: 23
Pages: 119-130
Related Report
Peer Reviewed
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[Journal Article] Identification of Toyocamycin, an agent cytotoxic for multiple myeloma cells, as a potent inhibitor of ER stress-induced XBP1 mRNA splicing.2012
Author(s)
Ri M, Tashiro E, Oikawa D, Shinjo S, Tokuda M, Yokouchi Y, Narita T, Masaki A, Ito A, Ding J, Kusumoto S, Ishida T, Komatsu H, Shiotsu Y, Ueda R, Iwawaki T, Imoto M, Iida S.
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Journal Title
Blood Cancer J.
Volume: 2
Issue: 7
Pages: e79-e79
DOI
Related Report
Peer Reviewed
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