| Project/Area Number |
23510291
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Resource conservation science
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| Research Institution | University of Yamanashi |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YAMAMURA Hideki 山梨大学, 医学工学総合研究部, 助教 (70516939)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 遺伝資源 / アクチノバクテリア / 生態学 / 分類学 / 微生物 |
|---|
| Research Abstract |
Actinobacteria are a group of Gram-positive and high GC content bacteria. The aim of the present study was to establish an effective isolation method for actinobacteria from river basin and evaluate their taxonomic diversity. By using a dilution plating method with 1/5 NBRC 802 medium containing 5% NaCl, a total of 194 actinobacteria were selectively isolated from the river and seashore sediments of Iriomote Island, Okinawa, Japan. According to the 16S rDNA homology search, they classified into 6 families, 10 genera and 23 species. Microbacterium thalassium was recognized as the most predominant species. Of 194 isolates, 48 strains were considered as belonging to new species. These results suggest that the river basin is a promising genetic resource for diverse and novel actinobacteria.
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