| Project/Area Number |
23550019
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Physical chemistry
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| Research Institution | Saga University |
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Principal Investigator |
UNNO Masashi 佐賀大学, 工学(系)研究科(研究院), 准教授 (50255428)
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| Co-Investigator(Renkei-kenkyūsha) |
KAMO Naoki 松山大学, 薬学部, 教授 (10001976)
MASUDA Shinji 東京工業大学, バイオ研究基盤支援総合センター, 准教授 (30373369)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 分子分光 / 振動分光 / 生物物理 / 光受容タンパク質 / 量子化学計算 / 国際研究者交流（米国） / 分子分光学 / 生物物理学 / 国際情報交流（米国） |
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| Research Abstract |
Many biological cofactors, such as light-absorbing chromophores in photoreceptors, are intrinsically planar molecules. A protein environment, however, causes structural distortions of the cofactor, and such structural changes can lead to a modulation of chemical properties of the cofactor to maximize its biological activity. We recently applied the near-infrared ROA to bacteriorhodopsin and photoactive yellow protein. These studies demonstrated that the near-infrared excitation allows us to measure the ROA spectra of a chromophore within a protein environment. Furthermore, calculations of the ROA spectra utilizing DFT provide detailed structural information, such as data about out-of-plane distortions of the chromophore. These findings extend the applicability of ROA to many biological cofactors.
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