Applications of Near-Infrared Raman Optical Activity to Biological Samples
| Project/Area Number |
23550019
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Physical chemistry
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| Research Institution | Saga University |
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Principal Investigator |
UNNO Masashi 佐賀大学, 工学(系)研究科(研究院), 准教授 (50255428)
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| Co-Investigator(Renkei-kenkyūsha) |
KAMO Naoki 松山大学, 薬学部, 教授 (10001976)
MASUDA Shinji 東京工業大学, バイオ研究基盤支援総合センター, 准教授 (30373369)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 分子分光 / 振動分光 / 生物物理 / 光受容タンパク質 / 量子化学計算 / 国際研究者交流（米国） / 分子分光学 / 生物物理学 / 国際情報交流（米国） |
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| Research Abstract |
Many biological cofactors, such as light-absorbing chromophores in photoreceptors, are intrinsically planar molecules. A protein environment, however, causes structural distortions of the cofactor, and such structural changes can lead to a modulation of chemical properties of the cofactor to maximize its biological activity. We recently applied the near-infrared ROA to bacteriorhodopsin and photoactive yellow protein. These studies demonstrated that the near-infrared excitation allows us to measure the ROA spectra of a chromophore within a protein environment. Furthermore, calculations of the ROA spectra utilizing DFT provide detailed structural information, such as data about out-of-plane distortions of the chromophore. These findings extend the applicability of ROA to many biological cofactors.
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Report
(4 results)
Research Products
(46 results)
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[Journal Article] Active Site Structure of Photoactive Yellow Protein with a Locked Chromophore Analogue Revealed by Near-Infrared Raman Optical Activity2013
Author(s)
Kubota, K., Shingae, T., Foster, N. D., Kumauchi, M., Hoff, W. D. & Unno, M
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Journal Title
J. Phys. Chem. Lett
Volume: 4
Pages: 3031-3038
Related Report
Peer Reviewed
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[Journal Article] Active Site Structure of Photoactive Yellow Protein with a Locked Chromophore Analogue Revealed by Near-Infrared Raman Optical Activity2013
Author(s)
Kubota, K., Shingae, T., Foster, N. D., Kumauchi, M., Hoff, W. D. & Unno, M.
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Journal Title
J. Phys. Chem. Lett.
Volume: 4
Issue: 18
Pages: 3031-3038
DOI
Related Report
Peer Reviewed
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[Journal Article] Spectroscopic Evidence for the Formation of an N Intermediate during the Photocycle of Sensory Rhodopsin II (Phoborhodopsin) from Natronobacterium pharaonis2011
Author(s)
Tateishi, Y., Abe, T., Tamogami, J., Nakao, Y., Kikukawa, T., Kamo, N., & Unno, M
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Journal Title
Biochemistry
Volume: 50
Pages: 2135-2143
Related Report
Peer Reviewed
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[Journal Article] Photochemistry of a Putative New Class of Sensory Rhodopsin (SRIII) coded by xop2 of Haloarcular marismortui2011
Author(s)
Nakao, Y., Kikukawa, T., Shimono1, K., Tamogami1, J., Kimitsuki, N., Nara, T., Unno, M., Ihara, K., & Kamo, N
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Journal Title
J. Photochem. Photobiol. B
Volume: 102
Pages: 45-54
Related Report
Peer Reviewed
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[Presentation] Oral, Active site structure of photoactive yellow protein with a locked chromophore analogue revealed by near infrared Raman optical activity2014
Author(s)
Shingae, T., Kubota, K., Foster, N. D., Kumauchi, M., Hoff, W., Unno, M
Organizer
1st International Picobiology Institute Symposium, Picometer- to Micrometer-Level Structural Analyses for Studies on Functional Mechanisms of Proteins
Place of Presentation
Hyogo
Related Report
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[Presentation] Active site structure of photoactive yellow protein with a locked chromophore analogue revealed by near infrared Raman optical activity2014
Author(s)
Shingae, T., Kubota, K., Foster, N. D., Kumauchi, M., Hoff, W., Unno, M.
Organizer
1st International Picobiology Institute Symposium, Picometer- to Micrometer-Level Structural Analyses for Studies on Functional Mechanisms of Proteins
Place of Presentation
Hyogo
Related Report
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[Presentation] Active Site Structure of Photoactive Yellow Protein with a Locked Chromophore Analog Revealed by Near Infrared Raman Optical Activity2013
Author(s)
Shingae, T., Kubota, K., Foster, N. D., Kumauchi, M., Hoff, W. D., Unno, M
Organizer
7th International Conference on Advanced Vibrational Spectroscopy
Place of Presentation
Kobe
Related Report
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