| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Research Abstract |
It is generally known that the reactions catalyzed by glycosyltransferases are highly specific. The enzymes strictly discriminate their own substrates and properly act on the certain position of the substrate, thereby allowing assemble of specific structure of oligosaccharide. This study was conducted to investigate the reverse reactions in which glycosytransferases remove monosaccharide from sugar chains. The removal should be sufficiently specific comparably to the forward reaction, transfer of sugar. Using alpha1,6fucosyltransferase as a model, we examined effects of various factors on the equilibrium of the reversible reaction of the glycosyltrasferase.
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