Oligosaccharide degradation based on the reverse reaction by glycosyltrasnferase

• Project/Area Number: 23550099
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Saga University
• Principal Investigator: TEI Bungyoku (IKEDA Yoshitaka) 佐賀大学, 医学部, 教授 (60252657)
• Co-Investigator(Renkei-kenkyūsha): IHARA Hideyuki 佐賀大学, 医学部, 助教 (50452834)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 糖鎖 / フコース転移酵素 / 糖ヌクレオチド / 逆反応 / 糖鎖構造 / 構造決定 / 糖転移酵素

Research Abstract

It is generally known that the reactions catalyzed by glycosyltransferases are highly specific. The enzymes strictly discriminate their own substrates and properly act on the certain position of the substrate, thereby allowing assemble of specific structure of oligosaccharide. This study was conducted to investigate the reverse reactions in which glycosytransferases remove monosaccharide from sugar chains. The removal should be sufficiently specific comparably to the forward reaction, transfer of sugar. Using alpha1,6fucosyltransferase as a model, we examined effects of various factors on the equilibrium of the reversible reaction of the glycosyltrasferase.

Research Products

• [Journal Article] An assay for alpha1,6-fucosyltransferase (FUT8) activity based on the HPLC separation of a reaction product with fluorescence detection. (2013)
  • Author(s): Ihara H, Tsukamoto H, Taniguchi N, Ikeda Y
  • Journal Title: Methods Mol. Biol. Volume: 1022 Pages: 335-348
  • DOI: 10.1007/978-1-62703-465-4_25
  • ISBN: 9781627034647, 9781627034654
  • Peer Reviewed
• [Journal Article] MD-2-dependent human Toll-like receptor 4 monoclonal antibodies detect extracellular association of Toll-like receptor 4 with extrinsic soluble MD-2 on the cell surface. (2013)
  • Author(s): Tsukamoto H, Ihara H, Ito R, Ukai I, Suzuki N, Kimoto M, Tomioka Y, Ikeda Y
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 440 Issue: 1 Pages: 31-36
  • DOI: 10.1016/j.bbrc.2013.09.004
  • Peer Reviewed
• [Journal Article] Reduced surface expression of TLR4 by a V254I point mutation accounts for the low lipopolysaccharide responder phenotype of BALB/c B cells. (2013)
  • Author(s): Tsukamoto H, Fukudome K, Takao S, Tsuneyoshi N, Ohta S, Nagai Y, Ihara H, Miyake K, Ikeda Y, Kimoto M
  • Journal Title: J. Immunol. Volume: 190 Issue: 1 Pages: 195-204
  • DOI: 10.4049/jimmunol.1201047
  • Peer Reviewed
• [Journal Article] Suppression of heregulin beta signaling by single N-glycan deletion mutant of soluble ErbB3 (2013)
  • Author(s): Takahashi M, Hasegawa Y, Ikeda Y, Wada Y, Tajiri M, Ariki S, Takamiya R, Nishitani C, Araki M, Yamaguchi Y, Taniguchi N, Kuroki Y.
  • Journal Title: J Biol Chem Volume: 288 Issue: 46 Pages: 32910-32921
  • DOI: 10.1074/jbc.m113.491902
  • Peer Reviewed
• [Journal Article] Difucosylation of chitooligosaccharides by eukaryote and prokaryote alpha1,6-fucosyltransferases. (2013)
  • Author(s): Ihara H, Hanashima S, Tsukamoto H, Yamaguchi Y, Taniguchi N, Ikeda Y
  • Journal Title: Biochim. Biophys. Acta Volume: 1830 Issue: 10 Pages: 4482-4490
  • DOI: 10.1016/j.bbagen.2013.05.013
  • Peer Reviewed
• [Journal Article] Multiple potential regulatory sites of TLR4 activation induced by LPS as revealed by novel inhibitory human TLR4 mAbs. (2012)
  • Author(s): Tsukamoto H, Fukudome K, Takao S, Tsuneyoshi N, Ihara H, Ikeda Y, Kimoto M
  • Journal Title: Int. Immunol. Volume: 24 Issue: 8 Pages: 495-506
  • DOI: 10.1093/intimm/dxs053
  • Peer Reviewed
• [Journal Article] Measurement of Peroxiredoxin-4 in Rat Tissues and Implication of Its Serum Levels as a Potential Marker for Hepatic Disease. (2012)
  • Author(s): Ritsu Ito
  • Journal Title: Mol Med Report Volume: 6 Issue: 2 Pages: 379-384
  • DOI: 10.3892/mmr.2012.935
  • Peer Reviewed
• [Presentation] 糖転移酵素GnT-V の神経幹細胞における特異的発現と神経再生への関与 (2013)
  • Author(s): 浜之上誠, 池田義孝, 緒方 徹, 高松 研
  • Organizer: 第36回日本分子生物学会年会
• [Presentation] Cloning, expression and characterization of silkworm a1, 6-fucosyltransferase (2013)
  • Author(s): 井原秀之, 池田義孝
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] 分裂期神経幹細胞特異的に限定的に発現する糖転移酵素GnT-V (2013)
  • Author(s): 浜之上誠, 池田義孝, 高松 研
  • Organizer: Neuro 2013(第36回日本神経科学大会・第56回日本神経化学大会)
• [Presentation] Cloning, expression and characterization of silkworm a1,6-fucosyltransferase. (2013)
  • Author(s): 井原 秀之，池田 義孝
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] 分裂期神経幹細胞特異的に限定的に発現する糖転移酵素GnT-V． (2013)
  • Author(s): 浜之上 誠，池田 義孝，高松 研
  • Organizer: Neuro 2013（第36回日本神経科学大会・第56回日本神経化学大会）
  • Place of Presentation: 京都
• [Presentation] 糖転移酵素GnT-Vの神経幹細胞における特異的発現と神経再生への関与． (2013)
  • Author(s): 浜之上 誠，池田 義孝，緒方 徹，高松 研
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] Reduced surface expression of TLR4 by a V254I point mutation may account for the low LPS responder phenotype of BALB/c B cells (2012)
  • Author(s): Tsukamoto H, Fukudome K, Takao S, Tsuneyoshi N, Ohta S, Nagai Y, Ihara H, Miyake K, Ikeda Y, Kimoto M
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
• [Presentation] ヒト及び根瘤菌α 1, 6-fucosyltransferase を用いたジフコシル化キトオリゴ糖の合成 (2012)
  • Author(s): 井原秀之, 花島慎弥, 塚本宏樹, 山口芳樹, 谷口直之, 池田義孝
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
• [Presentation] ヒト及び根瘤菌α1,6-fucosyltransferaseを用いたジフコシル化キトオリゴ糖の合成 (2012)
  • Author(s): 井原秀之、花島慎弥、塚本宏樹、山口芳樹、谷口直之、池田義孝
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
• [Presentation] 骨粗鬆症スクリーニング検査としての尿中γ-GTP濃度およびFRAX の有用性 (2011)
  • Author(s): 田中伸哉, 森脇佐和子, 上西一弘, 濃沼信夫, 田中清, 池田義孝, 新飯田俊平
  • Organizer: 第13回日本骨粗鬆症学会
  • Place of Presentation: 神戸
  • Year and Date: 2011-11-03
• [Presentation] 神経幹細胞における糖転移酵素の発現と機能解析 (2011)
  • Author(s): 浜之上誠, 池田義孝, 高松研
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
• [Presentation] The effectiveness of urinary gamma-glutamyltranspeptidase as a screening for osteoporosis (2011)
  • Author(s): Tanaka S, Moriwaki S, Uenishi K, Koinuma N, Tanaka K, Ikeda Y, Niida S
  • Organizer: The American Society for Bone and Mineral Research, Annual Meeting
  • Place of Presentation: California, USA
• [Presentation] The availability of urinary gamma-Glutamyltransferase as a screening for osteoporosis (2011)
  • Author(s): Tanaka S, Moriwaki S, Uenishi K, Koinuma N, Tanaka K, Ikeda Y, Niida S
  • Organizer: The 3rd Joint Meeting of ECTS & IBMS
  • Place of Presentation: Athens, Greece
• [Presentation] 骨粗鬆症スクリーニング検査としての尿中γ-GTP濃度およびFRAXの有用性 (2011)
  • Author(s): 田中伸哉，森脇佐和子，上西一弘，濃沼信夫，田中清，池田義孝，新飯田俊平
  • Organizer: 第13回日本骨粗鬆症学会
  • Place of Presentation: 神戸
• [Book] Fucosyltransferase 8. GDP-fucose N-glycan core α 6-fucosyltransferase (FUT8). In : Handbook of Glycosyltransferases and Related Genes 2nd ed. Taniguchi, N., Honke, K., Fukuda, M., Narimatsu, H., Yamaguchi, Y., Angata, T. (Eds.) (2014)
  • Author(s): Ihara H, Tsukamoto H, Gu J, Miyoshi E, Taniguchi N, Ikeda Y
  • Publisher: Springer
• [Book] Mannosyl (beta-1, 4-)-glycoprotein beta-1, 4-Nacetylglucosaminyltransferase (MGAT3) ; β 1, 4-N- Acetylglucosaminyltransferase III (GnT-III, GlcNAc-T III). In : Handbook of Glycosyltransferases and Related Genes 2nd ed. Taniguchi, N., Honke, K., Fukuda, M., Narimatsu, H., Yamaguchi, Y., Angata, T. (Eds.) (2014)
  • Author(s): Ikeda Y, Ihara H, Tsukamoto H, Gu J, Taniguchi N
  • Publisher: Springer