The study of specific reactions of hydrogen radical under ambient conditions

• Project/Area Number: 23550101
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Yokohama City University
• Principal Investigator: TAKAYAMA Mitsuo 横浜市立大学, その他の研究科, 教授 (10328635)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: 水素ラジカル / オゾン / 質量分析 / ビラジカル / 活性化学種 / 大気圧微小プラズマ

Research Abstract

To examine the reaction characteristics of hydrogen radicals under ambient conditions, we have made a generator of hydrogen radicals and estimated its performance. The hydrogen radical generator consists of a reaction cell of hydrogen gas and an ultraviolet lump of 160-400nm (3.8-7.7eV) in wavelength. The generation of hydrogen fluoride (HF) was confirmed with a mass spectrometer combined with an atmospheric pressure corona discharged ionization source. The mass spectrum showed the peaks corresponding to negative cluster ions F-(HF)n originating from the reactions of hydrogen radicals with Teflon wall of the reaction cell.

Research Products

• [Journal Article] Ionization characteristics of amino acids in direct analysis in real time mass spectrometry (2014)
  • Author(s): K. Sekimoto, M. Sakakura, T. Kawamukai, H. Hike, T. Shiota, F. Usui, Y. Bando and M. Takayama
  • Journal Title: Analyst Volume: 139 Pages: 2589-2599
  • Peer Reviewed
• [Journal Article] Analysis of Flexibility of Proteins by means of Positive and Negative Ion MALDI In-Source Decay Mass Spectrometry (2014)
  • Author(s): R. Iimuro and M. Takayama
  • Journal Title: Mass Spectrometry Volume: 3 Issue: Special_Issue Pages: S0023-S0023
  • DOI: 10.5702/massspectrometry.S0023
  • NAID: 130004727167
  • ISSN: 2186-5116, 2187-137X
  • Peer Reviewed
• [Journal Article] Selective and Nonselective Cleavages in Positive and Negative CID of the Fragments generated from In-Source Decay of Intact Proteins in MALDI-MS (2014)
  • Author(s): M. Takayama, S. Sekiya, R. Iimuro, S. Iwamoto, and K. Tanaka
  • Journal Title: J. Am. Soc. Mass Spectrom (Tokyo) Volume: 25 Pages: 120-131
  • Peer Reviewed
• [Journal Article] Selective and Nonselective Cleavages in Positive and Negative CID of the Fragments generated from In-Source Decay of Intact Proteins in MALDI-MS (2014)
  • Author(s): M. Takayama, S. Sekiya, R. Iimuro, S. Iwamoto, and K. Tanaka
  • Journal Title: J. Am. Soc. Mass Spectrom. Volume: 25 Pages: 120-131
  • Peer Reviewed
• [Journal Article] Influence of Initial Velocity of Analytes on In-source Decay Products in MALDI Mass Spectrometry using Salicylic Acid Derivative Matrices (2014)
  • Author(s): D. Asakawa, M. Sakakura, and M. Takayama
  • Journal Title: Int. J. Mass Spectrom. Volume: 337 Pages: 29-33
  • Peer Reviewed
• [Journal Article] Influence of Initial Velocity of Analytes on In-source Decay Products in MALDI Mass Spectrometry using Salicylic Acid Derivative Matrices (2013)
  • Author(s): D. Asakawa, M. Sakakura, and M. Takayama
  • Journal Title: Int. J. Mass Spectrom Volume: 337 Pages: 29-33
  • Peer Reviewed
• [Journal Article] Collision-Induced Dissociation Study of the Adduct Ions Produced in NO₃⁻-Free Area of Atmospheric Pressure Negative Corona Discharges under Ambient Air Conditions (2013)
  • Author(s): K. Sekimoto, N. Matsuda, M. Takayama
  • Journal Title: Mass Spectrometry Volume: 2 Issue: 1 Pages: A0020-A0020
  • DOI: 10.5702/massspectrometry.A0020
  • NAID: 130003380161
  • ISSN: 2186-5116, 2187-137X
  • Peer Reviewed
• [Journal Article] Collision-induced dissociation analysis of negative atmospheric ion adducts in atmospheric pressure corona discharge ionization mass spectrometry (2013)
  • Author(s): K. Sekimoto and M. Takayama
  • Journal Title: J. Am. Soc. Mass Spectrom Volume: 24 Pages: 780-788
  • Peer Reviewed
• [Journal Article] 5-Amino-1-naphthol, a novel 1, 5-naphthalene derivative matrix suitable for MALDI in-source decay of phosphorylated peptides (2013)
  • Author(s): I. Osaka, M. Sakai and M. Takayama
  • Journal Title: Rapid Commun. Mass Spectrom Volume: 27 Pages: 103-108
  • Peer Reviewed
• [Journal Article] 5-Amino-1-naphthol, a novel 1,5-naphthalene derivative matrix suitable for MALDI in-source decay of phosphorylated peptides (2013)
  • Author(s): I. Osaka, M. Sakai and M. Takayama
  • Journal Title: Rapid Commun. Mass Spectrom. Volume: 27 Pages: 103-108
  • Peer Reviewed
• [Journal Article] The pH Dependence of Product Ion Spectra Obtained from Precursor Ions with the Same Charge Number in ESI of Carbonic Anhydrase 2 (2013)
  • Author(s): Y. Nabuchi, K.Hirose and M.Takayama
  • Journal Title: Mass Spectrometry Volume: 2 Issue: 1 Pages: A0016-A0016
  • DOI: 10.5702/massspectrometry.A0016
  • NAID: 130003380158
  • ISSN: 2186-5116, 2187-137X
  • Peer Reviewed
• [Journal Article] Collision-induced dissociation analysis of negative atmospheric ion adducts in atmospheric pressure corona discharge ionization mass spectrometry (2013)
  • Author(s): K. Sekimoto and M. Takayama
  • Journal Title: J. Am. Soc. Mass Spectrom. Volume: 24 Issue: 5 Pages: 780-788
  • DOI: 10.1007/s13361-013-0576-2
  • Peer Reviewed
• [Journal Article] Influence of amino acid composition and phosphorylation on the ion yields of peptides in MALDI-MS (2012)
  • Author(s): D.Asakawa, S.Moriguchi and M.Takayama
  • Journal Title: J. Am. Soc. Mass Spectrom Volume: 23 Pages: 108-115
  • Peer Reviewed
• [Journal Article] Improved sonolytic hydrolysis of peptides in aqueous solution with addition of 1,4-benzenedithiol (2012)
  • Author(s): M.Sakakura and M.Takayama
  • Journal Title: Ultrasonics Sonochemistry Volume: 19 Pages: 615-620
  • Peer Reviewed
• [Journal Article] Mass spectrometric characterization of phosphorylated peptides using MALDI in-source decay via redox reactions (2012)
  • Author(s): D.Asakawa and M.Takayama
  • Journal Title: J. Mass Spectrom. Volume: 47 Pages: 180-187
  • Peer Reviewed
• [Journal Article] Fragmentation Processes of hydrogen-Deficient Peptide Radicals in Matrix-Assisted Laser Desorption/Ionization In-Source Decay Mass Spectrometry (2012)
  • Author(s): D.Asakawa and M.Takayama
  • Journal Title: J. Phys. Chem. B Volume: 116 Pages: 4016-4023
  • Peer Reviewed
• [Journal Article] Specific interaction between negative atmospheric ions and organic compounds in atmospheric pressure corona discharge ionization mass spectrometry (2012)
  • Author(s): K.Sekimoto, M.Sakai and M.Takayama
  • Journal Title: J. Am. Soc. Mass Spectrom. Volume: 23 Pages: 1109-1119
  • Peer Reviewed
• [Journal Article] Influence of Secondary Structure on In-Source Decay of Protein in Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (2012)
  • Author(s): M.Takayama, I.Osaka and M.Sakakura
  • Journal Title: Mass Spectrometry Volume: 1 Issue: 1 Pages: A0001-A0001
  • DOI: 10.5702/massspectrometry.A0001
  • NAID: 10031126337
  • ISSN: 2186-5116, 2187-137X
  • Peer Reviewed
• [Journal Article] Matrix Effect on In-Source Decay Products of Peptides in Matrix-Assisted Laser Desorption/Ionization (2012)
  • Author(s): D. Asakawa, M. Sakakura, M. Takayama
  • Journal Title: Mass Spectrometry Volume: 1 Issue: 1 Pages: A0002-A0002
  • DOI: 10.5702/massspectrometry.A0002
  • NAID: 130001937210
  • ISSN: 2186-5116, 2187-137X
  • Peer Reviewed
• [Journal Article] Influence of Charge State of Precursor Ions on Collision-induced Dissociation of Carbonic Anhydrase 2 (2012)
  • Author(s): Y.Nabuchi, K.Hirose and M.Takayama
  • Journal Title: J. Mass Spectrom. Soc. Jpn, Volume: 60 Pages: 47-50
  • NAID: 10031126335
  • Peer Reviewed
• [Journal Article] Surface analysis of traces stained with discharge on plane electrode in atmospheric pressure negative corona (2012)
  • Author(s): K.Sekimoto and M.Takayama
  • Journal Title: Journal of Electrostatics Volume: 70 Pages: 363-373
  • Peer Reviewed
• [Journal Article] Flexible Xxx&#8211;Asp/Asn and Gly&#8211;Xxx Residues of Equine Cytochrome <i>c</i> in Matrix-Assisted Laser Desorption/Ionization In-Source Decay Mass Spectrometry (2012)
  • Author(s): M. Takayama
  • Journal Title: Mass Spectrometry Volume: 1 Issue: 2 Pages: A0007-A0007
  • DOI: 10.5702/massspectrometry.A0007
  • NAID: 10031126344
  • ISSN: 2186-5116, 2187-137X
  • Peer Reviewed
• [Journal Article] Mass spectrometric characterization of phosphorylated peptides using MALDI in-source decay via redox reactions (2012)
  • Author(s): D.Asakawa and M.Takayama
  • Journal Title: J. Mass Spectrom Volume: 47 Pages: 180-187
  • Peer Reviewed
• [Journal Article] Temperature dependence of magic number and first hydrated shell of various core water cluster ions Y-(H2O)n (Y= O2, HOx, NOx, COx) in atmospheric pressure negative corona discharge mass spectrometry (2011)
  • Author(s): K.Sekimoto and M.Takayama
  • Journal Title: Int. J. Mass Spectrom Volume: 306 Pages: 44-50
  • Peer Reviewed
• [Journal Article] Specific cleavage at peptide backbone Cα-C and CO-N bonds during MALDI-ISD mass spectrometry with 5-nitrosalicylic acid as the matrix (2011)
  • Author(s): D.Asakawa and M.Takayama
  • Journal Title: Rapid Commun. Mass Spectrom. Volume: 25 Pages: 2379-2383
  • Peer Reviewed
• [Journal Article] Observation of different core water cluster ions Y-(H2O)n (Y=O2, HOx, NOx, COx) and magic number in atmospheric pressure corona discharge mass spectrometry (2011)
  • Author(s): K.Sekimoto and M.Takayama
  • Journal Title: J. Mass Spectrom Volume: 46 Pages: 50-60
  • Peer Reviewed
• [Journal Article] Cα-C bond cleavage of the peptide backbone in MALDI in-source decay using salicylic acid derivative matrices (2011)
  • Author(s): D.Asakawa and M.Takayama
  • Journal Title: J. Am. Soc. Mass Spectrom Volume: 22 Pages: 1224-1233
  • Peer Reviewed
• [Presentation] 大気圧下での負イオン付加体の生成と水素引き抜き反応 (2013)
  • Author(s): 松田菜津季, 関本奏子, 高山光男
  • Organizer: 第61回質量分析総合討論会
  • Place of Presentation: つくば
• [Presentation] 大気イオン生成に与えるオゾンの影響 (2013)
  • Author(s): 三舩千佳, 関本奏子, 高山光男
  • Organizer: 第61回質量分析総合討論会
  • Place of Presentation: つくば
• [Presentation] Molecular mass determination using collision-induced dissociation of negative atmospheric ion adducts generated in ambient corona discharges (2013)
  • Author(s): Kanako Sekimoto and Mitsuo Takayama
  • Organizer: American Mass Spectrometry Conference
  • Place of Presentation: Mineapollis
• [Presentation] 大気イオン生成に与えるオゾンの影響 (2013)
  • Author(s): 三舩千佳・関本奏子・高山光男
  • Organizer: 質量分析総合討論会
  • Place of Presentation: つくば
• [Presentation] 大気圧下での負イオン付加体の生成と水素引き抜き反応 (2013)
  • Author(s): 松田菜津季・関本奏子・高山光男
  • Organizer: 質量分析総合討論会
  • Place of Presentation: つくば
• [Presentation] Influence of Charge State of Precursor Ions on Collision-Induced Dissociation of Carbonic Anhydrase 2 (2012)
  • Author(s): Yoshiaki Nabuchi; Kenji Hirose ; Mitsuo Takayama
  • Organizer: the 60th ASMS Conference on Mass Spectrometry and Allied Topics
  • Place of Presentation: Vancouver, Canada
• [Presentation] Application of negative atmospheric ions formed in corona discharges to specific analyte ionization under ambient conditions (2012)
  • Author(s): Kanako Sekimoto and Mitsuo Takayama
  • Organizer: the 60th ASMS Conference on Mass Spectrometry and Allied Topics
  • Place of Presentation: Vancouver, Canada
  • Invited
• [Presentation] Optimization of matrix preparation methods for MALDI-ISD of protein (2012)
  • Author(s): Ryunosuke Iimuro, Mitsuo Takayama
  • Organizer: the 19th International Mass Spectrometry Conference
  • Place of Presentation: Kyoto
• [Presentation] Positive and negative ion formation of amino acid and its hydrated clusters using ambient corona discharge with micro-devices (2011)
  • Author(s): Mami Sakai; Mitsuo Takayama
  • Organizer: the 59th ASMS Conference on Mass Spectrometry and Allied Topics
  • Place of Presentation: Denver, USA
• [Presentation] Ion formation and evolution in atmospheric pressure corona discharges between arbitrary point-to-plane electrode conditions (2011)
  • Author(s): Kanako Sekimoto and Mitsuo Takayama
  • Organizer: the 59th ASMS Conference on Mass Spectrometry and Allied Topics
  • Place of Presentation: Denver, USA
• [Presentation] Cα-C bond cleavage of the peptide backbone in MALDI in-source decay using salicylic acid derivative matrices (2011)
  • Author(s): Daiki Asakawa; Mitsuo Takayama
  • Organizer: the 59th ASMS Conference on Mass Spectrometry and Allied Topics
  • Place of Presentation: Denver, USA
• [Presentation] Negative ion formation and evolution in atmospheric pressure corona discharges between point-to-plane electrodes with arbitrary configurations (2011)
  • Author(s): Kanako Sekimoto and Mitsuo Takayama
  • Organizer: VI International Workshop on Microplasmas
  • Place of Presentation: Paris, France
• [Presentation] Negative ion formation and evolution in atmospheric pressure corona discharges between point-to-plane electrodes with arbitrary configurations (2011)
  • Author(s): Kanako Sekimoto and Mitsuo Takayama
  • Organizer: the 30th International Conference on Phenomena in Ionized Gases
  • Place of Presentation: Belfast, Northern Ireland
• [Presentation] Radical-induced fragmentation of phosphorylated-peptides in MALDI MS (2011)
  • Author(s): Mitsuo Takayama, Motoshi Sakakura, Daiki Asakawa
  • Organizer: 2nd Asia and Oceanic Mass Spectrometry Conference(招待講演)
  • Place of Presentation: Busan, Korea
• [Presentation] ハードイオン化からソフトイオン化まで-その特性と応用の実際- (2011)
  • Author(s): 高山光男
  • Organizer: 第５回日本化学会関東支部大会(招待講演)
  • Place of Presentation: 東京農工大学・小金井キャンパス
• [Presentation] 水素引き抜き反応によるMALDIインソース分解のメカニズムおよびリン酸化プロテオームへの応用 (2011)
  • Author(s): 浅川大樹・高山光男
  • Organizer: 第59回質量分析総合討論会
  • Place of Presentation: ホテル阪急エキスポパーク・大坂
• [Book] 現代質量分析学 (2013)
  • Author(s): 高山光男, 他編
  • Publisher: 化学同人
• [Book] 現代質量分析学 (2013)
  • Author(s): 高山光男 他編
  • Total Pages: 514
  • Publisher: 化学同人
• [Book] 現代質量分析学 (2013)
  • Author(s): 高山光男、早川滋雄、瀧浪欣彦、和田芳直
  • Total Pages: 514
  • Publisher: 化学同人
• [Book] 翻訳後修飾のプロテオミクス (2011)
  • Author(s): 平野久・大野茂男編著 高山光男（分担）
  • Total Pages: 233
  • Publisher: 講談社
• [Remarks]
  • URL: http://masspecs.sci.yokohama-cu.ac.jp/
• [Remarks]
  • URL: http://masspecs.sci.yokohama-cu.ac.jp/
• [Patent(Industrial Property Rights)] 大気圧コロナ放電イオン化システム及びイオン化方法 (2011)
  • Inventor(s): 高山光男・関本奏子
  • Industrial Property Rights Holder: 高山光男・関本奏子
  • Filing Date: 2011
• [Patent(Industrial Property Rights)] タンパク質とリン酸化ペプチドのペプチド主鎖のN-Ca結合又はCa-C結合の特異的切断方法 (2011)
  • Inventor(s): 高山光男・大坂一生
  • Industrial Property Rights Holder: 高山光男・大坂一生
  • Filing Date: 2011