Development of real sample preparation method for biosensing using magnetic separation

• Project/Area Number: 23550109
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Okayama University of Science
• Principal Investigator: NAGATANI Naoki 岡山理科大学, 工学部, 准教授 (90351072)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: バイオテクノロジー / バイオセンサー / 試料処理 / 分離分析 / 磁性微粒子 / 磁気分離 / バイオチップ / バイオセンサ / 電気化学測定 / イムノクロマト法

Research Abstract

The purpose of this research aims for high sensitivity biosensing by pretreatment of the measurement sample using magnetic particles. The pretreatment using magnetic particles is effective in separation and concentration for protein and genomic DNA. Separation and concentration are possible without using a centrifuge by biochip technology and magnetic microparticles, and the pretreatment processes of the measurement sample can be reduced. Furthermore, rapid and sensitive detection can be possible by using immunochromatography method for biochip technology and magnetic particles.

Research Products

• [Journal Article] Detection of influenza virus using a lateral flow immunoassay for amplified DNA by a microfluidic RT-PCR chip (2012)
  • Author(s): Naoki Nagatani, Keiichiro Yamanaka, Hiromi Ushijima, Ritsuko Koetsu, Tadahiro Sasaki, Kazuyoshi Ikuta, Masato Saito, Toshiro Miyahara, Eiichi Tamiya
  • Journal Title: Analyst Volume: 137 Pages: 3422-3426
  • Peer Reviewed
• [Presentation] Gold nanoparticles based-biosensor for amplified DNA, The 4th Asian Symposium on Advanced Materials-Chemistry (2013)
  • Author(s): Naoki Nagatani, Yutaka Ogido, Keiichiro Yamanaka, Masato Saito, Eiichi Tamiya, Seiichi Katayama, Toshiro Miyahara
  • Organizer: Physics & Biomedicine of Functional and Novel Material
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2013-10-24
• [Presentation] 磁性微粒子による唾液中タンパク質の分離と検出 (2013)
  • Author(s): 永谷尚紀, 味能直弘, 荻堂 裕, 牛島ひろみ, 宮原敏郎
  • Organizer: 電気化学会第79回大会
  • Place of Presentation: 浜松
  • Year and Date: 2013-03-31
• [Presentation] Gold nanoparticles based-biosensor for amplified DNA (2013)
  • Author(s): Naoki Nagatani, Yutaka Ogido, Keiichiro Yamanaka, Masato Saito, Eiichi Tamiya, Seiichi Katayama, Toshiro Miyahara
  • Organizer: The 4th Asian Symposium on Advanced Materials: Chemistry, Physics & Biomedicine of Functional and Novel materials (ASAM-4)
  • Place of Presentation: Taiwan, Taipei
• [Presentation] Detection of Amplified DNA Using Gold Nanoparticles Based-Biosensor (2013)
  • Author(s): Naoki Nagatani, Keiichiro Yamanaka, Masato Saito, Eiichi Tamiya, Toshiro Miyahara
  • Organizer: Bio4Apps 2013
  • Place of Presentation: 東京、東京医科歯科大学
• [Presentation] Detection of E.coli using electrochemical and immunochromatographic assay for amplified gene by PCR (2012)
  • Author(s): Yutaka Ogido, Hiromi Ushijima, Keiichiro Yamanak, Masato Saito, Eiichi Tamiya, Toshiro Miyahara, Naoki Nagatani
  • Organizer: Pacific Rim Meeting on Electrochemical and Solid-State Science 2012
  • Place of Presentation: Honolulu, Hawaii, USA
• [Presentation] 磁性微粒子による唾液中タンパク質の分離と検出
  • Author(s): 永谷尚紀、味能直弘、荻堂裕、牛島ひろみ、宮原敏郎
  • Organizer: 電気化学会７９回大会
  • Place of Presentation: アクトシティ浜松（浜松市中区板屋町111-1)