Phenotyping of cell through the glycolipid metabolic analyssis

• Project/Area Number: 23550185
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Chemistry related to living body
• Research Institution: Hokkaido University
• Principal Investigator: FUJITANI Naoki 北海道大学, 先端生命科学研究科(研究院), 特任助教 (10374191)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 糖脂質 / 質量分析 / メタボローム解析 / 質量分析法 / 核磁気共鳴法

Research Abstract

Characterization of cells was performed by the comprehensive and quantitative analyses of glycosphingolipids which are ubiquitously expressed in all kinds of cell. Since glycolipids are the representative of secondary metabolites, the comprehensive analysis is intended to indicate briefly the state of the cell. Quantitative structural analyses were performed using mass spectrometry. In order to simplify the distribution of molecular masses of glycosphingolipids (elimination of structural diversities), enzymatic reaction system to digest efficiently of ceramide moieties in glycosphingolipids was established, enabling to analyze the glycan parts only. As a result, the expression level and structure of glycosphingolipids of each cell is highly cell-specific, and statistical analysis of the quantitative data showed that it makes it possible to classify and delineate the cells efficiently.

Research Products

• [Journal Article] Total cellular glycomics allows characterizing cells and streamlining the discovery process for cellular biomarkers (2013)
  • Author(s): Fujitani, N., Furukawa, J., 他10名
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 110 Issue: 6 Pages: 2105-2110
  • DOI: 10.1073/pnas.1214233110
  • NAID: 120005326735
  • Peer Reviewed
• [Journal Article] Recent advances in cellular glycomic analyses (2013)
  • Author(s): Furukawa, J., Fujitani, N., Shinohara, Y.
  • Journal Title: Biomolecules Volume: 3 Issue: 1 Pages: 198-225
  • DOI: 10.3390/biom3010198
  • Peer Reviewed
• [Journal Article] QualitativeandQuantitative Cellular Glycomics of Glycosphingolipids Based on Rhodococcal Endoglycosylceramidase-assisted Glycan Cleavage, Glycoblotting-assisted Sample Preparation, and Matrix-assisted Laser Desorption Ionization Tandem Time-of-flight Mass Spectrometry Analysis (2011)
  • Author(s): Naoki Fujitani, Yasuhiro Takegawa, Yohei Ishibashi, Kayo Araki, Jun-ichi Furukawa, Susumu Mitsutake, Yasuyuki Igarashi, Makoto Ito, Yasuro Shinohara
  • Journal Title: THE JOURNAL OF BIOLOGICAL CHEMISTRY Volume: 286 Issue: 48 Pages: 41669-41679
  • DOI: 10.1074/jbc.m111.301796
  • Peer Reviewed
• [Presentation] 総合的な複合糖質糖鎖プロファイリングによる細胞の分類とバイオマーカー探索 (2012)
  • Author(s): 藤谷直樹、古川潤一, 他5名
  • Organizer: 第31回日本糖質学会年会
  • Place of Presentation: 鹿児島市民文化ホール(鹿児島市)
• [Presentation] 総合的な複合糖質糖鎖プロファイリングによる細胞の分類とバイオマーカー探索 (2012)
  • Author(s): 藤谷直樹、古川潤一、荒木香代、 藤岡剛、伊東信、中村幸夫、篠原康郎
  • Organizer: 第31回日本糖質学会年会
  • Place of Presentation: 鹿児島市民文化ホール（鹿児島市）
• [Presentation] スフィンゴ糖脂質のグライコミクスによる細胞の解析・定義 (2011)
  • Author(s): 藤谷直樹、石橋洋平, 他6名
  • Organizer: 第4回セラミド研究会
  • Place of Presentation: 北海道大学学術交流会館(札幌市)
• [Presentation] スフィンゴ糖脂質のグライコミクスによる細胞の解析・定義 (2011)
  • Author(s): 藤谷直樹、荒木香代, 他3名
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都国際会議場(京都市)
• [Presentation] スフィンゴ糖脂質のグライコミクスによる細胞の解析・定義 (2011)
  • Author(s): 藤谷直樹、石橋洋平、荒木香代、古川潤一、光武進、五十嵐靖之、伊東信、篠原康郎
  • Organizer: 第4回セラミド研究会
  • Place of Presentation: 北海道大学（札幌市）
• [Presentation] スフィンゴ糖脂質のグライコミクスによる細胞の解析・定義 (2011)
  • Author(s): 藤谷直樹、荒木香代、古川潤一、伊東信、篠原康郎
  • Organizer: 第84回日本生化学会大会 2011/9/21~24
  • Place of Presentation: 京都国際会議場（京都市）
• [Patent(Industrial Property Rights)] Method for selecting pluripotent stem cell (2012)
  • Inventor(s): Shinohara, T., Furukawa, J., Fujitani, N., Araki, K., Nakamura, Y.
  • Industrial Property Rights Holder: Shinohara, T., Furukawa, J., Fujitani, N., Araki, K., Nakamura, Y.
  • Industrial Property Rights Type: 特許
  • Filing Date: 2012-11-05
  • Overseas