| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
In this study, we have examined novel type reactions of enzymatic heme degradation. A recent in vitro study has revealed that a canonical heme degrading enzyme, heme oxygenase (HO), produces a new heme catabolite under special reaction condition. The new catabolite was successfully detected from mammalian cell culture, suggesting that the novel HO reaction can proceed in living organisms. On the other hand, newly-found IsdG-type enzymes degraded heme into unique reaction products through distinct reaction mechanisms. The mechanistic difference from the well-studied HO reaction appears to be caused by unusual non-planarity of heme bound to the IsdG-type enzymes. These diversity shed new light on enzymatic heme degradation to stimulate biological research exploring new physiological functions as well as its mechanistic studies.
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