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Structure analysis and characterization of the LH1-RC complex induced by metal ions

Research Project

Project/Area Number 23550188
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Chemistry related to living body
Research InstitutionIbaraki University

Principal Investigator

OTOMO Seiu  茨城大学, 理学部, 教授 (10213612)

Co-Investigator(Kenkyū-buntansha) MIKI Kunio  京都大学, 理学研究科, 教授 (10116105)
KIMURA Yukihiro  神戸大学, 農学研究科, 助教 (20321755)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords光合成 / 光捕集 / 反応中心 / 光電変換 / 色素膜タンパク質 / 金属イオン / 生体分子認識 / 生体機能 / 光捕集複合体
Research Abstract

The crystal structure of a bacterial photosynthetic light-harvesting-reaction center core complex (LH1-RC) has been determined. The structure reveals a closed arrangement of LH1 complex around the RC, and the LH1 BChl a molecules form a partially overlapping ring with a shorter Mg-Mg spacing compared with that of B850 in LH2. Structural evidence is for first time provided for the possible ubiquinone pathway in the closed LH1 complex. The Ca2+-binding sites are identified. Molecular mechanisms of quinone transport, Ca2+-regulation and interaction between LH1 and RC are described below. The result has been published in Nature.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (27 results)

All 2014 2013 2012 2011 Other

All Journal Article (11 results) (of which Peer Reviewed: 11 results) Presentation (14 results) Remarks (2 results)

  • [Journal Article] Structure of the LH1-RC complex from Thermochromatium tepidum at 3.0 Å2014

    • Author(s)
      Niwa, S., Yu, L.-J., Takeda, K., Hirano, Y., Kawakami, T., Wang-Otomo, Z.-Y. & Miki, K.
    • Journal Title

      Nature

      Volume: 508 Pages: 228-232

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structure of the LH1-RC complex from Thermochromatium tepidum at 3.0 A2014

    • Author(s)
      Niwa, S., Yu, L.-J., Takeda, K., Hirano, Y., Kawakami, T., Wang-Otomo, Z.-Y. & Miki, K.
    • Journal Title

      Nature

      Volume: 508 Issue: 7495 Pages: 228-232

    • DOI

      10.1038/nature13197

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ATR-FTIR detection of the metal-sensitive structural changes in the light-harvesting 1 reaction center complex from the thermophilic purple sulfur bacterium Thermochromatium tepidum2013

    • Author(s)
      Li, Y., Kimura, Y., Arikawa, T., Wang-Otomo, Z.-Y. & Ohno, T.
    • Journal Title

      Biochemistry

      Volume: 52 Pages: 9001-9008

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structure analysis and characterization of the cytochrome c-554 from thermophilic green sulfur photosynthetic bacterium Chloroba- culum tepidum2013

    • Author(s)
      Yu, L.-J., Unno, M., Kimura, Y., Yanagimoto, K., Oh-Oka, H., Wang-Otomo, Z.-Y.
    • Journal Title

      Photosynth. Res.

      Volume: 118 Pages: 249-258

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structure analysis and comparative characterization of the cytochrome c'and flavocytochrome c from thermophilic purple photosynthetic bacterium Thermochromatium tepidum2012

    • Author(s)
      Hirano, Y., Kimura, Y., Suzuki, H., Miki, K. & Wang(Otomo), Z.-Y.
    • Journal Title

      Biochemistry

      Volume: 51 Pages: 6556-6567

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Metal cations modulate the bacteriochlorophyll- protein interaction in the light-harvesting 1 core complex from Thermochromatium tepidum2012

    • Author(s)
      Kimura, Y., Inada, Y., Numata, T., Arikawa, T., Li, Y., Zhang, J.-P., Wang(Otomo), Z.-Y. & Ohno, T.
    • Journal Title

      Biochim. Biophys. Acta

      Volume: 1817 Pages: 1022-1029

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gene sequencing and characterization of the light-harvesting complex 2 from thermophilic purple sulfur bacterium Thermochromatium tepidum2012

    • Author(s)
      Sekine, F., Horiguchi, K., Kashino, Y., Shimizu, Y., Yu, L.-J., Kobayashi, M. & Wang(Otomo), Z.-Y.
    • Journal Title

      Photosynth. Res.

      Volume: 111 Pages: 9-18

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Metal cations modulate the bacteriochlorophyll-protein interaction in the light-harvesting 1 core complex from Thermochromatium tepidum2012

    • Author(s)
      Y. Kimura et al.
    • Journal Title

      Biochim. Biophys. Acta

      Volume: 1817 Issue: 7 Pages: 1022-1029

    • DOI

      10.1016/j.bbabio.2012.03.016

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Structure analysis and comparative characterization of the cytochrome c’ and flavocytochrome c from thermophilic purple photosynthetic bacterium Thermochromatium tepidum2012

    • Author(s)
      Y. Hirano, et al.
    • Journal Title

      Biochemistry

      Volume: 51 Issue: 33 Pages: 6556-6567

    • DOI

      10.1021/bi3005522

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] A spectroscopic variant of the light-harvesting 1 core complex from the thermophilic purple sulfur bacterium Thermochromatium tepidum2011

    • Author(s)
      Y. Kimura et al.
    • Journal Title

      Biochemistry

      Volume: 50 Pages: 3638-3648

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effect of aggregation on the excitation dynamics of LH2 from Thermochromatium tepidum in aqueous phase and in chromatophores2011

    • Author(s)
      F. Yang, et al.
    • Journal Title

      J. Phys. Chem. B

      Volume: 115 Pages: 7906-7913

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Crystal structure of a LH1-RC core complex from Thermochromatium tepidum2013

    • Author(s)
      Z.-Y. Wang-Otomo, L.-J. Yu, S. Niwa, K. Takeda, Y. Hirano, T. Kawakami, K. Miki
    • Organizer
      16th International Congress on Photosynthesis Research(第16回国際光合成会議)
    • Place of Presentation
      St. Louis, MO, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] 好熱紅色細菌の光捕集反応中心複合体の機能解明と構造解析2013

    • Author(s)
      于龍江、竹田一旗、平野優、丹羽智美、川上知朗、大友征宇、三木邦夫
    • Organizer
      第21回光合成セミナー
    • Place of Presentation
      名工大
    • Related Report
      2013 Final Research Report
  • [Presentation] 好熱紅色細菌の光捕集反応中心複合体の機能解明と構造解析2013

    • Author(s)
      于龍江、竹田一旗、平野優、丹羽智美、川上知朗、○大友征宇、三木邦夫
    • Organizer
      第21回光合成セミナー
    • Place of Presentation
      名工大
    • Related Report
      2013 Annual Research Report
  • [Presentation] Crystal structure of a LH1-RC core complex from Thermochromatium tepidum2013

    • Author(s)
      ○大友征宇、于龍江、丹羽智美、竹田一旗、平野優、川上知朗、三木邦夫
    • Organizer
      第16回国際光合成会議
    • Place of Presentation
      米国St. Louis
    • Related Report
      2013 Annual Research Report
  • [Presentation] 紅色硫黄細菌由来光捕集1複合体における色素―蛋白質間相互作用の解析2012

    • Author(s)
      木村行宏、永麗、有川曜央、沼田朋子、大友征宇、大野隆
    • Organizer
      第20回光合成セミナー
    • Place of Presentation
      阪大
    • Year and Date
      2012-06-30
    • Related Report
      2013 Final Research Report
  • [Presentation] 紅色細菌の光捕集タンパク質のリン酸化2012

    • Author(s)
      日下部広和、清水佑記、于龍江、大友征宇
    • Organizer
      第20回光合成セミナー
    • Place of Presentation
      阪大
    • Year and Date
      2012-06-30
    • Related Report
      2013 Final Research Report
  • [Presentation] 好熱性紅色硫黄細菌Thermochromatium tepidum由来光捕集1複合体におけるBChl-aとTrp残基間の水素結合相互作用2012

    • Author(s)
      L. Yong, Y. Kimura, T. Numata, Y. Inada, T. Arikawa, S. Otomo, T. Ohno
    • Organizer
      日本生物物理学会
    • Place of Presentation
      名大
    • Related Report
      2013 Final Research Report
  • [Presentation] 紅色細菌の光捕集タンパク質のリン酸化2012

    • Author(s)
      大友征宇 et al.
    • Organizer
      光合成セミナー
    • Place of Presentation
      大阪大学豊中キャンパス
    • Related Report
      2012 Research-status Report
  • [Presentation] 紅色硫黄細菌由来光捕集1複合体における色素―蛋白質間相互作用の解析2012

    • Author(s)
      木村行宏 et al.
    • Organizer
      光合成セミナー
    • Place of Presentation
      大阪大学豊中キャンパス
    • Related Report
      2012 Research-status Report
  • [Presentation] Hydrogen-bonding interactions between BChl-a and Trp residues in the light-harvesting 1 complexes from Thermochromatium tepidum2012

    • Author(s)
      Y. Li et al.
    • Organizer
      生物物理学会
    • Place of Presentation
      名古屋大学
    • Related Report
      2012 Research-status Report
  • [Presentation] 好熱性光合成細菌の光捕集複合体LH1の単離精製と特性評価2012

    • Author(s)
      川上知朗 et al.
    • Organizer
      日本化学会関東支部茨城地区交流会
    • Place of Presentation
      日立シビックセンター
    • Related Report
      2012 Research-status Report
  • [Presentation] 紅色硫黄光合成細菌の光捕集複合体LH2の特性評価2011

    • Author(s)
      大友征宇 et al.
    • Organizer
      第19回光合成の色素系と反応中心に関するセミナー
    • Place of Presentation
      大阪大学豊中キャンパス
    • Related Report
      2011 Research-status Report
  • [Presentation] 光合成硫黄細菌の光捕集反応中心複合体の単離精製と結晶化2011

    • Author(s)
      藤田智史 et al.
    • Organizer
      日本化学会関東支部茨城地区交流会
    • Place of Presentation
      いばらき量子ビーム研究センター
    • Related Report
      2011 Research-status Report
  • [Presentation] 光合成細菌の光捕集複合体LH2の特性評価2011

    • Author(s)
      日下部広和 et al.
    • Organizer
      日本化学会関東支部茨城地区交流会
    • Place of Presentation
      いばらき量子ビーム研究センター
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://biophys.sci.ibaraki.ac.jp/

    • Related Report
      2013 Final Research Report
  • [Remarks] 大友研ホームページ

    • URL

      http://biophys.sci.ibaraki.ac.jp

    • Related Report
      2013 Annual Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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