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Development of polymer additive that prevents protein aggregation and inactivation

Research Project

Project/Area Number 23550189
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Chemistry related to living body
Research InstitutionUniversity of Tsukuba

Principal Investigator

SHIRAKI Kentaro  筑波大学, 数理物質系, 准教授 (90334797)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsタンパク質 / 凝集 / 高分子 / 溶液添加剤 / 失活 / 添加剤 / フォールディング
Research Abstract

Small additives have been developed that prevents protein aggregation and inactivation. Here, we have developed the polymers that prevent protein aggregation and protein stabilization, and also we have examined those molecular mechanisms. The molecular mechanism of polyethylene glycol as an aggregation suppressor was different mechanism from that of the well-used additive arginine hydrochloride. It was found that cationic polyallylamine and anionic polyacrylic acid bind on protein that stabilizes protein structure in aqueous solution. PEGylated polymer was more favorable as aggregation suppressor for aggregation-prone protein.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (15 results)

All 2014 2013 2012 2011

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (5 results)

  • [Journal Article] Cysteine inhibits the fibrillisation and cytotoxicity of amyloid-β 40 and 42 : implications for the contribution of the thiophilic interaction2014

    • Author(s)
      Eisuke Takai, Ken Uda, Tomonori Yoshida, Tamotsu Zako, Mizuo Maeda, Kentaro Shiraki
    • Journal Title

      Physical Chemistry Chemical Physics

      Volume: 16 Issue: 8 Pages: 3566-3572

    • DOI

      10.1039/c3cp54245a

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Degeneration of amyloid-β fibrils by low temperature atmospheric pressure plasma in solution2014

    • Author(s)
      Eisuke Takai, Gai Ohashi, Tomonori Yoshida, Karin Margareta Sorgjerd, Tamotsu Zako, Mizuo Maeda, Katsuhisa Kitano, Kentaro Shiraki
    • Journal Title

      Applied Physics Letters

      Volume: 104 Issue: 2 Pages: 23701-23701

    • DOI

      10.1063/1.4861842

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular Dynamics Simulation of Arginine-Assisted Solubilization of Caffeic Acid : Intervention in the Interaction2013

    • Author(s)
      Atsushi Hirano, Tomoshi Kameda, Daisuke Shinozaki, Tsutomu Arakawa, Kentaro Shiraki
    • Journal Title

      J. Phys. Chem

      Volume: B117 Issue: 25 Pages: 7518-7528

    • DOI

      10.1021/jp401609p

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Arginine inhibits adsorption of proteins on polystyrene surface.2013

    • Author(s)
      Yui Shikiya, Shunsuke Tomita, Tsutomu Arakawa, Kentaro Shiraki
    • Journal Title

      PLoS ONE

      Volume: 8 Issue: 8 Pages: e70762-e70762

    • DOI

      10.1371/journal.pone.0070762

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Oligoethylene glycols prevent thermal aggregation of α-chymotrypsin in a temperature-dependent manner : Implications for design guidelines.2013

    • Author(s)
      Shunsuke Tomita, Yumiko Tanabe, Kentaro Shiraki
    • Journal Title

      Biotechnology Progress

      Volume: 27 Issue: 5 Pages: 1325-1330

    • DOI

      10.1002/btpr.1762

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Synergistic solubilization of porcine myosin in physiological salt solution by arginine2013

    • Author(s)
      Eisuke Takai, Shunsuke Yoshizawa, Daisuke Ejima, Tsutomu Arakawa, Kentaro Shiraki
    • Journal Title

      International Journal of Biological Macromolecules

      Volume: 62 Pages: 647-651

    • DOI

      10.1016/j.ijbiomac.2013.09.035

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mechanistic insights into protein precipitation by alcohol2012

    • Author(s)
      Hiroki Yoshikawa, Atsushi Hirano, Tsutomu Arakawa, Kentaro Shiraki
    • Journal Title

      International Journal of Biological Macromolecules

      Volume: 50 Issue: 3 Pages: 865-871

    • DOI

      10.1016/j.ijbiomac.2011.11.005

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Adsorption and disruption of lipid bilayers by nanoscale protein aggregates2012

    • Author(s)
      Atsushi Hirano, Hiroki Yoshikawa, Shuhei Matsushita, Yoichi Yamada, Kentaro Shiraki
    • Journal Title

      Langmuir

      Volume: 28 Issue: 8 Pages: 3887-3895

    • DOI

      10.1021/la204717c

    • Related Report
      2013 Final Research Report 2012 Research-status Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Improved Complementary Polymer Pair System: Switching for Enzyme Activity by PEGylated Polymers2012

    • Author(s)
      Takaaki Kurinomaru, Shunsuke Tomita, Shinpei Kudo, Sumon Ganguli, Yukio Nagasaki, Kentaro Shiraki
    • Journal Title

      Langmuir

      Volume: Vol.28, No.9 Issue: 9 Pages: 4334-4338

    • DOI

      10.1021/la2043312

    • Related Report
      2013 Final Research Report 2012 Research-status Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Different mechanisms of action of poly(ethylene glycol) and arginine on thermal inactivation of lysozyme and ribonuclease A2012

    • Author(s)
      Shunsuke Tomita
    • Journal Title

      Biotechnology and Bioengineering

      Volume: 109 Issue: 10 Pages: 2543-2552

    • DOI

      10.1002/bit.24531

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] ポリスチレン表面へのタンパク質の吸着とアルギニンによる吸着の抑制2012

    • Author(s)
      志喜屋惟、冨田峻介、白木賢太郎
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2013 Final Research Report
  • [Presentation] 高分子電解質を利用したa-キモトリプシン超活性化系の作成2012

    • Author(s)
      栗之丸隆章、冨田峻介、白木賢太郎
    • Organizer
      第12回日本蛋白質科学会年会
    • Place of Presentation
      名古屋国際会議場
    • Related Report
      2013 Final Research Report
  • [Presentation] ポリスチレン表面へのタンパク質の吸着とアルギニンによる吸着の抑制2012

    • Author(s)
      志喜屋惟、冨田峻介、白木賢太郎
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡国際会議場(福岡)
    • Related Report
      2012 Research-status Report
  • [Presentation] Superactivation of α-chymotrypsin by poly(allylamine)2012

    • Author(s)
      Takaaki Kurinomaru, Shunsuke Tomita, and Kentaro Shiraki.
    • Organizer
      第12回日中韓酵素工学会議
    • Place of Presentation
      金沢エクセルホテル東急(石川)
    • Related Report
      2012 Research-status Report
  • [Presentation] オリゴエチレングリコール誘導体によるタンパク質凝集の抑制2011

    • Author(s)
      田邊裕美子、冨田峻介、白木賢太郎
    • Organizer
      高分子討論会第26回高分子学会関東支部茨城地区若手の会交流会
    • Place of Presentation
      つくばみらい市
    • Related Report
      2013 Final Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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