| Project/Area Number |
23550195
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
TERUYA Kenta 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (30372288)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | リポソーム / 縮合 / 蛋白質 / 細胞表面 / α-チオエステル / ファチジルエタノール / C末端修飾 / 緑色蛍光蛋白質 / チオエステル / コレステロール |
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| Research Abstract |
In our previous study, semi-synthesis of protein specifically modified with lipo-philic compounds was carry out, but not effective. Denaturing protein during the specific condensation, purification of coupling product, and toxicity to live cell are to be adapted for improving effectively. To overcome these difficulties, liposome was employed in each step. As results, separation of coupling product from reaction mixture of condensation, and condensation avoiding denature of protein were carried out smoothly. However, use of liposome in coupling without detergent was not effective. Andmore, release of protein from the liposeme was not effective when liposeme horboring modified protein was applied to living cell.
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