Enhancement by liposome of semi-synthesis of specifically modified protein at the C-terminal and application to living cell
Project/Area Number |
23550195
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Chemistry related to living body
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Research Institution | Kyoto Prefectural University of Medicine |
Principal Investigator |
TERUYA Kenta 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (30372288)
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Project Period (FY) |
2011 – 2013
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
|
Keywords | リポソーム / 縮合 / 蛋白質 / 細胞表面 / α-チオエステル / ファチジルエタノール / C末端修飾 / 緑色蛍光蛋白質 / チオエステル / コレステロール |
Research Abstract |
In our previous study, semi-synthesis of protein specifically modified with lipo-philic compounds was carry out, but not effective. Denaturing protein during the specific condensation, purification of coupling product, and toxicity to live cell are to be adapted for improving effectively. To overcome these difficulties, liposome was employed in each step. As results, separation of coupling product from reaction mixture of condensation, and condensation avoiding denature of protein were carried out smoothly. However, use of liposome in coupling without detergent was not effective. Andmore, release of protein from the liposeme was not effective when liposeme horboring modified protein was applied to living cell.
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Site-specific isotope labeling of long RNA for structural and mechanistic studies.2012
Author(s)
3. Kawahara I, Haruta K, Ashihara Y, Yamanaka D, Kuriyama M, Toki N, Kondo Y, Teruya K, Ishikawa J, Furuta H, Ikawa Y, Kojima C, Tanaka Y.
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Journal Title
Nucleic Acids Research
Volume: 40
Issue: 1
Pages: e7-e7
DOI
Related Report
Peer Reviewed
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[Journal Article] Binding and selectivity of the marine toxins neodysiherbaine A, and its synthetic analogues, to GluK1 and GIuK2 kainate receptors2011
Author(s)
M.Unno, M.Shinohara, K.Takayama, H.Tanaka, K.Teruya, K.Doh-ura, R.Sakai, M.Sasaki, M.Ikeda-Saito
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Journal Title
Journal of Molecular Biology
Volume: 413
Issue: 3
Pages: 667-683
DOI
Related Report
Peer Reviewed
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[Presentation] Evaluation of Hydroxymethylcarbonyl- and Hydroxyethylamine-type BACE1 Inhibiors Containing a Superior BACE1 Cleavage Sequence.2013
Author(s)
Kazuya Kobayashi, Tomomi Akiyama, Yukie Nohara, Saki Goto, Mami Okabe, Natsumi Sato, Miki Itoh, Kanami Ueno, Yasunao Hattori, Kenta Teruya, Akira Sanjho, Kenichi Akaji.
Organizer
4th Asia-Pacific International Peptide Symposium (APIPS)
Place of Presentation
大阪
Related Report
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[Presentation] Syntheses of Hydroxyethylamine Derivatives Containing Side-Chain Mimetic and Peptide Sequence for BACE1 inhibitor2012
Author(s)
Hattori, Y.; Akiyama, T.; Nohara, Y.; Nishizaki, A.; Deguchi, A.; Shimamoto, Y.; Teruya, K.; Konnno, H.; Akaji, K.
Organizer
AIMECS11
Place of Presentation
Tokyo, Japan
Related Report
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[Presentation] Evaluation of Hydroxymethylcarbonyl- and Hydroxyethylamine-type BACE1 Inhibiors Containing a Superior BACE1 Cleavage Sequence
Author(s)
Kazuya Kobayashi, Tomomi Akiyama, Yukie Nohara, Saki Goto, Mami Okabe, Natsumi Sato, Miki Itoh, Kanami Ueno, Yasunao Hattori, Kenta Teruya, Akira Sanjho, Kenichi Akaji
Organizer
4th Asia-Pacific International Peptide Symposium (APIPS)
Place of Presentation
Osaka
Related Report
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