| Project/Area Number |
23550197
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
FUJII Teruo 東京大学, 生産技術研究所, 教授 (30251474)
YAMAMOTO Takatoki 東京工業大学, 大学院理工学研究科, 准教授 (20322688)
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| Project Period (FY) |
2011-04-28 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | DNA / PCR / 論理ゲート / GFP / 無細胞翻訳系 / プライマー / 論理演算 / 鋳型 |
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| Outline of Final Research Achievements |
Several PCR-based molecular logic gates were constructed. The logic gate was a PCR reaction mixture containing a linear DNA template (logic gate template) coding a green fluorescent protein (GFP) and a single stranded short DNA that hybridizes with the logic gate template (pre-mixed primer). Since the logic gate templates carry recognition sequences for transcriptional and translational machinery, the amplified GFP-coding sequence is translated to GFP in a coupled cell-free transcription-translation system, wired to the logic gates as a REPORT gate, that is, the output from the PCR-based logic gate is reported by green fluorescence. In this study, some PCR-based logic gates were designed to expand the variations in the logic operation pattern. Each gates designed and constructed on the study functioned properly.
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