Preparation and biofunctional evaluation of PNA-PEG conjugates that can be applied to the genetic diseases caused by single nucleotide mutations.
| Project/Area Number |
23550243
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Polymer/Textile materials
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| Research Institution | Tottori University |
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Principal Investigator |
SAKURAI Toshihiko 鳥取大学, 工学(系)研究科(研究院), 准教授 (10332868)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
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| Keywords | 人工核酸 / ペプチド核酸 / 遺伝子発現制御 / 1塩基認識 / 細胞内発現制御 / 細胞内輸送 / マクロピノサイトーシス / 細胞内輸送挙動 |
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| Research Abstract |
We synthesized the PNA-PEG conjugate and tried to regulate a gene expression using the cell free protein expression systems. As a result, the PNA-PEG conjugate inhibited the protein expression effectively and recognized the difference of the one base in vivo, compared with homo PNA oligomer. This was caused by the thermal stability at 30 degree, and this type of PNA-PEG conjugate was transported with macropinocytosis, but couldn't release to cytosol. with peptide signal (Q7-G-HSP), PNA-PEG conjugate was released to cytosol from mcropinosome, but it showed cytotoxicity that can not be ignored.
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Report
(4 results)
Research Products
(47 results)
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[Presentation] 櫻井 敏彦
Author(s)
遺伝子配列の１塩基の違いを見つけ出す人工遺伝子
Organizer
ＪＳＴ新技術説明会
Place of Presentation
JST東京別館ホール（東京）
Related Report
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