Development of autofocusing system with video-rate camera for electron tomography
| Project/Area Number |
23560024
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Thin film/Surface and interfacial physical properties
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
NISHI Ryuji 大阪大学, 超高圧電子顕微鏡センター, 准教授 (40243183)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YOSHIDA Kiyokazu 超高圧電子顕微鏡センター, 特任研究員 (50263223)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Keywords | 電子線トモグラフィー / 超高圧電子顕微鏡 / オートフォーカス / 自動撮影 / 画像鮮鋭度 / 鮮鋭度 |
|---|
| Research Abstract |
It is useful to observe the three-dimensional structure of medical and biological samples by using ultrahigh voltage electron microscope tomography. Taking several tens or more images with slightly different tilt angle of sample is necessary, however, a mechanical rotation accuracy is worse than of a few micrometers. Therefore, we accomplished fast taking of the tilt image series with automatic focusing and field position correction. To capture the 61 CCD images with a resolution of 4000 X 4000 pixels is completed in 30 minutes of half of conventional method.
|
Report
(4 results)
Research Products
(15 results)
