| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Research Abstract |
Motion of neutrophil-like HL60 cells under a pressing fore on HUVECs cultured on a flat glass plate was observed using the inclined centrifuge microscope. It was observed that the cells move along marginal regions of convex HUVECs. This suggests that the surface morphology of endothelium enhances possibility of adhesion between neutrophils and the endothelium because P-selectin which is necessary for neutrophils' rolling is extensively expressed on the marginal region of the endothelial cells. The HL60 cells move more straight on oriented HUVECs which had been exposed to a shear stress than on not-oriented HUVECs, but their mean velocity was not changed with the orientation. Since the mean velocity of undifferentiated HL60 cells increased on the oriented HUVECs, adhesivity of the differentiated HL60 cells to the HUVECs is considered to be a dominant factor to regulate the mean velocity.
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