The improvement effect of xenon gas on cellular viability for cryopreservation of biological tissue
| Project/Area Number |
23560241
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Thermal engineering
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 低温保存 / 凍結保存 / キセノンガス / 細胞障害 / 保護効果 / 細胞損傷 / ヒト皮膚繊維芽細胞 |
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| Research Abstract |
The purpose of this task was to clarify the efficiency of a xenon gas in order to reduce the damage by the intercellular stress during freezing and the cellular damage by the low temperature itself under an unfrozen condition in cryopreservation of a sample with a level of thick biological tissue. First, the enhanced effect of reduction of cellular damage in freezing process of a cell with a combination of dimethylsulfoxide as a typical cryoprotectant and pressurized dissolution and decompression of xenon gas was examined. And then the protective effect to the cell against the stress of the low temperature itself at 4 degree Celsius was examined. As a result, when a sample was frozen at a low cooling rate, it was suggested that dissolution of xenon gas was effective in reduction of damage by the intercellular stress, and it was clear that pressurized addition of xenon gas was effective in reduction of the cellular damage by the low temperature itself under an unfrozen condition.
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Report
(4 results)
Research Products
(10 results)
