| Project/Area Number |
23560394
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Electron device/Electronic equipment
|
|---|
| Research Institution | Toyohashi University of Technology |
|---|
Principal Investigator |
ISHII Hiromu 豊橋技術科学大学, テーラーメイド・バトンゾーン教育推進本部, 特任教授 (20506175)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
KATSUYUKI Machida 東京工業大学, 総合理工学研究科, 連携教授 (90597676)
SHIN-ICHI Yoshida 九州大学, 医学研究院, 教授 (60128113)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
|
|---|
| Keywords | 集積化MEMS / 細菌 / レジオネラ / マイクロ流路 / マイクロビーズ / 蛍光 / MEMS / PDMS / 葉酸 |
|---|
| Research Abstract |
Sensing bacteria is attracting a great deal of interest to avoid an outbreak of bacterial infection. The sensors used for this purpose should integrate at least two functions in a small volume: trapping bacteria and detecting them. We developed a PDMS-based microfluidic chip for trapping and detecting L. pneumophila cells. By using our finding that L. pneumophila might emit fluorescence when they are forced to limit their motion in a narrow space, we can observe fluorescence from L. pneumophila cells introduced into the chip together with microbeads: We previously make a mixed suspension of L. pneumophila cells and the beads and then introduce them into the microfluidic chip with funnel-shaped protrusions which work as stoppers both for the cells and the beads. The cells are densely packed at the inlet side of the stoppers and restricted their motion confined by microbeads. This method enables us to expect the observation of fluorescence from the L. pneumophila cells introduced.
|
