Fundamental Research on MEMS-based Bacterial Sensor
| Project/Area Number |
23560394
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Electron device/Electronic equipment
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| Research Institution | Toyohashi University of Technology |
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Principal Investigator |
ISHII Hiromu 豊橋技術科学大学, テーラーメイド・バトンゾーン教育推進本部, 特任教授 (20506175)
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| Co-Investigator(Renkei-kenkyūsha) |
KATSUYUKI Machida 東京工業大学, 総合理工学研究科, 連携教授 (90597676)
SHIN-ICHI Yoshida 九州大学, 医学研究院, 教授 (60128113)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Keywords | 集積化MEMS / 細菌 / レジオネラ / マイクロ流路 / マイクロビーズ / 蛍光 / MEMS / PDMS / 葉酸 |
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| Research Abstract |
Sensing bacteria is attracting a great deal of interest to avoid an outbreak of bacterial infection. The sensors used for this purpose should integrate at least two functions in a small volume: trapping bacteria and detecting them. We developed a PDMS-based microfluidic chip for trapping and detecting L. pneumophila cells. By using our finding that L. pneumophila might emit fluorescence when they are forced to limit their motion in a narrow space, we can observe fluorescence from L. pneumophila cells introduced into the chip together with microbeads: We previously make a mixed suspension of L. pneumophila cells and the beads and then introduce them into the microfluidic chip with funnel-shaped protrusions which work as stoppers both for the cells and the beads. The cells are densely packed at the inlet side of the stoppers and restricted their motion confined by microbeads. This method enables us to expect the observation of fluorescence from the L. pneumophila cells introduced.
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Report
(4 results)
Research Products
(25 results)
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[Presentation] PDMS-BASED MICROFLUIDIC CHIP FOR DETECTION OF LEGIONELLA PNEUMOPHILA BY MIXING WITH MICROBEAD2014
Author(s)
R. Hayashi, Y. Nishimura, H. Nakazawa, Ishida, K. Sawada, H.Ishii, K.Machida, Ishihara, K. Masu, C. Wang, K. Iida, M. Saito, S. Yoshida
Organizer
The 7th Asia-Pacific Conference on Transducers and Micro/Nano Technologies (APCOT)
Place of Presentation
Daegu, Korea(accepted for presentation)
Related Report
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[Presentation] PDMS-BASED MICROFLUIDIC CHIP FOR DETECTION OF LEGIONELLA PNEUMOPHILA BY MIXING WITH MICROBEADS2014
Author(s)
R. Hayashi, Y. Nishimura, H. Nakazawa, M. Ishida, K. Sawada, H. Ishii, K. Machida, N. Ishihara, K. Masu, C. Wang, K. Iida, M. Saito, and S. Yoshida
Organizer
The 7th Asia-Pacific Conference on Transducers and Micro/Nano Technologies (APCOT 2014)
Place of Presentation
Daegu Korea
Related Report
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[Presentation] Bacterial Diagnostic Chip by the Detection of Fluorescence from Legionella pneumophila in a Microbeads Suspension2014
Author(s)
R. Hayashi, H. Nakazawa, K. Sawada, M. Ishida, H. Ishii, K. Machida, C. Wang, K. Iida, M. Saito, and S. Yoshida
Organizer
225ｔｈ ECS Meeting, The Electrochemical Sciety
Place of Presentation
Orland USA
Related Report
Invited
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[Presentation] Bacterial Diagnostic Chip by the Detection of Fluorescence from Legionella pneumophila in a Microbeads Suspension2013
Author(s)
R. Hayashi, H. Nakazawa, K. Sawada, M. Ishida, H. Ishii, K. Machida, C. Wang, K. Iida, M. Saito, S. Yoshida
Organizer
Abs. Q2-1485, The 225th Electrochemical Society Spring Meeting
Place of Presentation
Orland USA
Related Report
Invited
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[Presentation] MEMS型細菌チップに捕獲されたレジオネラの 蛍光スペクトルと培養条件2013
Author(s)
林隆平, 中澤寛一, 石田誠, 澤田和明, 石井仁, 町田克之, 石原昇, 益一哉, 王上楽, 飯田健一郎, 齋藤光正, 藤井潤, 吉田眞一
Organizer
第30回センサ・マイクロマシンとその応用システム, 電気学会
Place of Presentation
仙台国際センター 仙台
Related Report
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[Presentation] Fabrication of Bio-MEMS Device for on-chip Testing of the Bacteria Behavior2012
Author(s)
Youngshik Shin, Kyohei Katsube, Kazuaki Sawada, Makoto Ishida, Hiromu Ishii, Katsuyuki Machida, Kazuya Masu, Ken-ichiro Iida, Mitsumasa Saito, Shin-ichi Yoshida
Organizer
Abstract No. B 5.3, Heterogeneous Integration Challenges of MEMS, Sensor, and CMOS LSI, Symp. B, 2012 MRS Spring Meeting, JSAP-MRS
Place of Presentation
San Francisco, USA
Related Report
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[Presentation] Bio-MEMES Chip for Trapping Bacteria by Si-pillar Structure and Its Application to Legionella2012
Author(s)
Kyohei Katsube, Ryuhei Hayashi, Youngshik Shin, Hirokazu Nakazawa, Makoto Ishida, Kazuaki Sawada, Hiromu Ishii, Katsuyuki Machida, Noboru Ishihara, Kazuya Masu, Yuta Takekawa, Changle Wang, Ken-ichiro Iida, Mitsumasa Saito, Jun Fujii, Shin-ichi Yoshida
Organizer
Abstract No. ac12000224, The 6th Asia-Pacific Conference on Transducers and Micro/Nano Technologies (APCOT)
Place of Presentation
Nanjing, China
Related Report
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[Presentation] Fabrication of Bio-MEMS Device for on-chip Testing of the Bacteria Behavior2012
Author(s)
Youngshik Shin, Kyohei Katsube, Kazuaki Sawada, Makoto Ishida, Hiromu Ishii, Katsuyuki Machida, Kazuya Masu, Ken-ichiro Iida, Mitsumasa Saito, and Shin-ichi Yoshida
Organizer
2012 MRS Spring Meeting, JSAP-MRS
Place of Presentation
San Francisco, USA
Related Report
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[Presentation] Bio-MEMES Chip for Trapping Bacteria by Si-pillar Structure and Its Application to Legionella2012
Author(s)
K. Katsube, R. Hayashi, Y. Shin, H. Nakazawa, M. Ishida, K. Sawada, H. Ishii, K. Machida, N. Ishihara, K. Masu, Y. Takekawa, C. Wang, K. Iida, M. Saito, J. Fujii, S. Yoshida
Organizer
The 6th Asia-Pacific Conference on Transducers and Micro/Nano Technologies (APCOT), IEEE
Place of Presentation
南京、中国
Related Report
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