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Fundamental Research on MEMS-based Bacterial Sensor

Research Project

Project/Area Number 23560394
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Electron device/Electronic equipment
Research InstitutionToyohashi University of Technology

Principal Investigator

ISHII Hiromu  豊橋技術科学大学, テーラーメイド・バトンゾーン教育推進本部, 特任教授 (20506175)

Co-Investigator(Renkei-kenkyūsha) KATSUYUKI Machida  東京工業大学, 総合理工学研究科, 連携教授 (90597676)
SHIN-ICHI Yoshida  九州大学, 医学研究院, 教授 (60128113)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Keywords集積化MEMS / 細菌 / レジオネラ / マイクロ流路 / マイクロビーズ / 蛍光 / MEMS / PDMS / 葉酸
Research Abstract

Sensing bacteria is attracting a great deal of interest to avoid an outbreak of bacterial infection. The sensors used for this purpose should integrate at least two functions in a small volume: trapping bacteria and detecting them. We developed a PDMS-based microfluidic chip for trapping and detecting L. pneumophila cells. By using our finding that L. pneumophila might emit fluorescence when they are forced to limit their motion in a narrow space, we can observe fluorescence from L. pneumophila cells introduced into the chip together with microbeads: We previously make a mixed suspension of L. pneumophila cells and the beads and then introduce them into the microfluidic chip with funnel-shaped protrusions which work as stoppers both for the cells and the beads. The cells are densely packed at the inlet side of the stoppers and restricted their motion confined by microbeads. This method enables us to expect the observation of fluorescence from the L. pneumophila cells introduced.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (25 results)

All 2014 2013 2012 2011 Other

All Journal Article (1 results) Presentation (21 results) (of which Invited: 6 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 1 results)

  • [Journal Article] Bio-MEMS Chip for Bacteria Detection -A Challenge of Si Technology to Biomedical Field-2013

    • Author(s)
      Hiromu Ishii, Kazuaki Sawada, Makoto Ishida, Katsuyuki Machida, Ken-Ichiro Iida, Mitsumasa Saito, Shin-ichi Yoshida
    • Journal Title

      ECS Trans.

      Volume: Vol. 58, No.9 Pages: 125-133

    • Related Report
      2013 Final Research Report
  • [Presentation] PDMS-BASED MICROFLUIDIC CHIP FOR DETECTION OF LEGIONELLA PNEUMOPHILA BY MIXING WITH MICROBEAD2014

    • Author(s)
      R. Hayashi, Y. Nishimura, H. Nakazawa, Ishida, K. Sawada, H.Ishii, K.Machida, Ishihara, K. Masu, C. Wang, K. Iida, M. Saito, S. Yoshida
    • Organizer
      The 7th Asia-Pacific Conference on Transducers and Micro/Nano Technologies (APCOT)
    • Place of Presentation
      Daegu, Korea(accepted for presentation)
    • Related Report
      2013 Final Research Report
  • [Presentation] Bacterial diagnostic microfluidic chip for detecting Legionella pneumophila2014

    • Author(s)
      Hiromu Ishii, Makoto Ishida, Kazuaki Sawada, Katsuyuki Machida, Kazuya Masu, Ken-ichiro Iida, Mitsumasa Saito, and Shin-ichi Yoshida
    • Organizer
      IEEE INEC2014
    • Place of Presentation
      Sapporo, Japan
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Bacterial diagnostic microfluidic chip for detecting Legionella pneumophila2014

    • Author(s)
      Hiromu Ishii, Makoto Ishida, Kazuaki Sawada, Katsuyuki Machida, Kazuya Masu, Ken-ichiro Iida, Mitsumasa Saito, and Shin-ichi Yoshida
    • Organizer
      The 6th IEEE International Nanoelectronics Conference (INEC 2014), IEEE
    • Place of Presentation
      北海道大学 札幌
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] PDMS-BASED MICROFLUIDIC CHIP FOR DETECTION OF LEGIONELLA PNEUMOPHILA BY MIXING WITH MICROBEADS2014

    • Author(s)
      R. Hayashi, Y. Nishimura, H. Nakazawa, M. Ishida, K. Sawada, H. Ishii, K. Machida, N. Ishihara, K. Masu, C. Wang, K. Iida, M. Saito, and S. Yoshida
    • Organizer
      The 7th Asia-Pacific Conference on Transducers and Micro/Nano Technologies (APCOT 2014)
    • Place of Presentation
      Daegu Korea
    • Related Report
      2013 Annual Research Report
  • [Presentation] Bacterial Diagnostic Chip by the Detection of Fluorescence from Legionella pneumophila in a Microbeads Suspension2014

    • Author(s)
      R. Hayashi, H. Nakazawa, K. Sawada, M. Ishida, H. Ishii, K. Machida, C. Wang, K. Iida, M. Saito, and S. Yoshida
    • Organizer
      225th ECS Meeting, The Electrochemical Sciety
    • Place of Presentation
      Orland USA
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] ビーズを用いたPDMS製細菌捕獲チップの検討2014

    • Author(s)
      林隆平,中澤寛一,西村祐典,石田誠,澤田和明,石井仁,町田克之,益一哉,飯田健一郎,齋藤光正,吉田眞一
    • Organizer
      第61回応用物理学会春季学術講演会
    • Place of Presentation
      青山学院大学 淵野辺
    • Related Report
      2013 Annual Research Report
  • [Presentation] Bio-MEMS Chip for Bacteria Detection–A Challenge of Si Technology to Biomedical Field-2013

    • Author(s)
      Hiromu Ishii, Makoto Ishida, Kazuaki Sawada, Katsuyuki Machida, Ken-ichiro Iida, Mitsumasa Saito, Shin-ichi Yoshida
    • Organizer
      Abs. E12-2222, The 224th Electrochemical Society Fall Meeting
    • Place of Presentation
      San Francisco, USA
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Bacterial Diagnostic Chip by the Detection of Fluorescence from Legionella pneumophila in a Microbeads Suspension2013

    • Author(s)
      R. Hayashi, H. Nakazawa, K. Sawada, M. Ishida, H. Ishii, K. Machida, C. Wang, K. Iida, M. Saito, S. Yoshida
    • Organizer
      Abs. Q2-1485, The 225th Electrochemical Society Spring Meeting
    • Place of Presentation
      Orland USA
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Bio-MEMS Chip for Bacteria Detection -A Challenge of Si Technology to Biomedical Field-2013

    • Author(s)
      Hiromu Ishii, Makoto Ishida, Kazuaki Sawada, Katsuyuki Machida, Ken-ichiro Iida, Mitsumasa Saito, and Shin-ichi Yoshida
    • Organizer
      224th ECS Meeting, The Electrochemical Sciety
    • Place of Presentation
      San Francisco USA
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] MEMS型細菌チップに捕獲されたレジオネラの 蛍光スペクトルと培養条件2013

    • Author(s)
      林隆平, 中澤寛一, 石田誠, 澤田和明, 石井仁, 町田克之, 石原昇, 益一哉, 王上楽, 飯田健一郎, 齋藤光正, 藤井潤, 吉田眞一
    • Organizer
      第30回センサ・マイクロマシンとその応用システム, 電気学会
    • Place of Presentation
      仙台国際センター 仙台
    • Related Report
      2013 Annual Research Report
  • [Presentation] 細菌迅速検出用マイクロチップV ‐培養条件と蛍光特性‐2013

    • Author(s)
      林隆平,中澤寛一,石田誠,澤田和明,石井仁,町田克之,石原昇,益一哉,王常楽,飯田健一郎,齋藤光正,藤井潤,吉田眞一
    • Organizer
      第74回応用物理学会秋季学術講演会
    • Place of Presentation
      同志社大学 京都
    • Related Report
      2013 Annual Research Report
  • [Presentation] Fabrication of Bio-MEMS Device for on-chip Testing of the Bacteria Behavior2012

    • Author(s)
      Youngshik Shin, Kyohei Katsube, Kazuaki Sawada, Makoto Ishida, Hiromu Ishii, Katsuyuki Machida, Kazuya Masu, Ken-ichiro Iida, Mitsumasa Saito, Shin-ichi Yoshida
    • Organizer
      Abstract No. B 5.3, Heterogeneous Integration Challenges of MEMS, Sensor, and CMOS LSI, Symp. B, 2012 MRS Spring Meeting, JSAP-MRS
    • Place of Presentation
      San Francisco, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] Bio-MEMES Chip for Trapping Bacteria by Si-pillar Structure and Its Application to Legionella2012

    • Author(s)
      Kyohei Katsube, Ryuhei Hayashi, Youngshik Shin, Hirokazu Nakazawa, Makoto Ishida, Kazuaki Sawada, Hiromu Ishii, Katsuyuki Machida, Noboru Ishihara, Kazuya Masu, Yuta Takekawa, Changle Wang, Ken-ichiro Iida, Mitsumasa Saito, Jun Fujii, Shin-ichi Yoshida
    • Organizer
      Abstract No. ac12000224, The 6th Asia-Pacific Conference on Transducers and Micro/Nano Technologies (APCOT)
    • Place of Presentation
      Nanjing, China
    • Related Report
      2013 Final Research Report
  • [Presentation] MEMS型細菌捕獲チップの製作とレジオネラを用いた評価2012

    • Author(s)
      林隆平,勝部恭平,辛永式,中澤寛一,石田誠,澤田和明,石井仁,町田克之,石原昇,益一哉,飯田健一郎,齋藤光正,藤井潤,吉田眞一
    • Organizer
      第29回センサ・マイクロマシンとその応用システム,電気学会
    • Place of Presentation
      小倉
    • Related Report
      2012 Research-status Report
  • [Presentation] Fabrication of Microfluidic Device for On-chip Observation of Bacterial Behavior2012

    • Author(s)
      Youngshik Shin, Kyohei Katsube, Makoto Ishida, Kazuaki Sawada, Hiromu Ishii, Katsuyuki Machida, Kazuya Masu, Ken-ichiro Iida, Mitsumasa Saito, and Shin-ichi Yoshida
    • Organizer
      第29回センサ・マイクロマシンとその応用システム,電気学会
    • Place of Presentation
      小倉
    • Related Report
      2012 Research-status Report
  • [Presentation] Fabrication of Bio-MEMS Device for on-chip Testing of the Bacteria Behavior2012

    • Author(s)
      Youngshik Shin, Kyohei Katsube, Kazuaki Sawada, Makoto Ishida, Hiromu Ishii, Katsuyuki Machida, Kazuya Masu, Ken-ichiro Iida, Mitsumasa Saito, and Shin-ichi Yoshida
    • Organizer
      2012 MRS Spring Meeting, JSAP-MRS
    • Place of Presentation
      San Francisco, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] Bio-MEMES Chip for Trapping Bacteria by Si-pillar Structure and Its Application to Legionella2012

    • Author(s)
      K. Katsube, R. Hayashi, Y. Shin, H. Nakazawa, M. Ishida, K. Sawada, H. Ishii, K. Machida, N. Ishihara, K. Masu, Y. Takekawa, C. Wang, K. Iida, M. Saito, J. Fujii, S. Yoshida
    • Organizer
      The 6th Asia-Pacific Conference on Transducers and Micro/Nano Technologies (APCOT), IEEE
    • Place of Presentation
      南京、中国
    • Related Report
      2012 Research-status Report
  • [Presentation] 細菌迅速検出用マイクロチップIII ‐細菌の捕獲状態と蛍光強度‐2012

    • Author(s)
      勝部 恭平,林 隆平,辛 永式,中澤 寛一,石田 誠,澤田 和明,石井 仁, 町田 克之,石原 昇,益 一哉,飯田 健一郎,齋藤 光正,藤井 潤,吉田 眞一
    • Organizer
      第59回応用物理学関係連合講演会
    • Place of Presentation
      早稲田大学
    • Related Report
      2011 Research-status Report
  • [Presentation] 細菌迅速検出用マイクロチップ IV ‐蛍光色素を用いた細菌検出条件の検討‐2012

    • Author(s)
      林 隆平,勝部恭平,中澤寛一,石田 誠,澤田和明,石井 仁,町田克之,石原 昇,益 一哉,飯田健一郎,齋藤光正,藤井 潤,吉田眞一
    • Organizer
      第59回応用物理学関係連合講演会
    • Place of Presentation
      早稲田大学
    • Related Report
      2011 Research-status Report
  • [Presentation] Fabrication of microfluidic device for the bacteria observation2012

    • Author(s)
      Youngshik Shin,Kyohei Katsube,Makoto Ishida,Kazuaki Sawada,Hiromu Ishii,Katsuyuki Machida,Noboru Ishihara,Kazuya Masu,Ken-ichiro Iida,Mistumasa Saito,Jun Fujii,Shin-ichi Yoshida
    • Organizer
      第59回応用物理学関係連合講演会
    • Place of Presentation
      早稲田大学
    • Related Report
      2011 Research-status Report
  • [Presentation] 細菌迅速検出用マイクロチップII ‐蛍光強度の細菌濃度依存性‐2011

    • Author(s)
      勝部 恭平,辛 永式,石田 誠,澤田 和明,石井 仁, 町田 克之,石原 昇,益 一哉, 飯田 健一郎,齋藤 光正,藤井 潤,吉田 眞一
    • Organizer
      第72回応用物理学会学術講演会
    • Place of Presentation
      山形大学
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.vbl.tut.ac.jp/top/houkoku/201106_report.pdf

    • Related Report
      2011 Research-status Report
  • [Patent(Industrial Property Rights)] 細菌検知方法, 細菌検査用デバイスおよび細菌検査装置2014

    • Inventor(s)
      中沢寛一, 石井仁, 林隆平, 澤田和明, 吉田眞一, 齋藤光正
    • Industrial Property Rights Holder
      国立大学法人 豊橋技術科学大学 国立大学法人 九州大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2014-026939
    • Filing Date
      2014-02-14
    • Related Report
      2013 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 細菌検知方法,細菌検査用デバイスおよび細菌検査装置2014

    • Inventor(s)
      中澤寛一,石井仁,林隆平,澤田和明,吉田眞一,齋藤光正
    • Industrial Property Rights Holder
      豊橋技術科学大学,九州大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2014-026939
    • Filing Date
      2014-02-14
    • Related Report
      2013 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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