Development of an immobilization method for cDNA on an electronic circuit and an application for a detection of mRNA
| Project/Area Number |
23560409
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Electron device/Electronic equipment
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| Research Institution | The University of Kitakyushu |
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Principal Investigator |
ISODA Takaaki 北九州市立大学, 国際環境工学部, 准教授 (70284544)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | DNA / 遺伝子 / タンパク / 電子回路 / バイオセンサー / DNAコンピューター / DANコンピューター / m-RNA / ｃ-DNA / 相互作用 / ｍ-RNA / c-DNA |
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| Research Abstract |
We have been established an electrochemical patterning method which is possible to control an immobilization of DNA or protein molecule by an electrochemical reaction on a microelectrode mounted on a circuit board. Patterning of protein on 4mm area has been achieved where 25 pair of microelectrodes of 200 micrometer was integrated. We also found that traditional cell-free protein synthesis method in a test tube using a template DNA could be progressed on a microelectrode mounted on a circuit board and an expression of a protein could be detected.
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Report
(4 results)
Research Products
(29 results)
