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Development of rapid screening method for viable microbes using ATP analysis in the course of dialysis water production process

Research Project

Project/Area Number 23560656
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Civil and environmental engineering
Research InstitutionNational Institute of Public Health

Principal Investigator

SIMAZAKI Dai  国立保健医療科学院, 生活環境研究部, 上席主任研究官 (60322046)

Co-Investigator(Kenkyū-buntansha) AKIBA Michihiro  国立保健医療科学院, 統括研究官 (00159336)
Project Period (FY) 2011-04-28 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords透析用水 / 衛生管理 / 生菌 / ATP / エンドトキシン / 細菌
Outline of Final Research Achievements

Applicability of adenosine triphosphate (ATP) assay for rapid screening of viable microbes in raw water and in the course of dialysis water production process was examined in this study. Samples were collected from Hospital A, where water for medical care was supplied by both public water supply and private water supply from groundwater. Viable microbes were robustly detected when ATP exceeded 5x10^-13mol/L, however, there was not significant correlation between total viable microbial count (TVC) and ATP. Twenty-fold concentration of the samples could increase detection sensitivity of ATP. The ATP assay would be considered as complementary to conventional TVC monitoring in terms of rapid screening of the presence of viable microbial contamination for ensuring microbial safety of dialysis water on a daily basis.

Report

(5 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (6 results)

All 2014 2013 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (5 results)

  • [Journal Article] 透析用水製造過程および原水におけるATP 測定による生菌迅速スクリーニング手法の検討2014

    • Author(s)
      島﨑大,秋葉道宏
    • Journal Title

      土木学会論文集G(環境)

      Volume: 70

    • NAID

      130004962641

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 透析用水製造過程および原水におけるATP 測定による生菌迅速スクリーニング手法の検討2014

    • Author(s)
      島﨑大,秋葉道宏
    • Organizer
      第51回土木学会環境工学研究フォーラム
    • Place of Presentation
      山梨大学甲府キャンパス
    • Year and Date
      2014-12-20 – 2014-12-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] Rapid screening of viable microbes in the course of dialysis water production process using adenosine triphosphate (ATP) assay2014

    • Author(s)
      Dai SIMAZAKI, Michihiro AKIBA
    • Organizer
      7th Congress of the International Society for Hemodialysis
    • Place of Presentation
      沖縄コンベンションセンター
    • Year and Date
      2014-04-25 – 2014-04-27
    • Related Report
      2014 Annual Research Report 2013 Research-status Report
  • [Presentation] 透析用水製造過程におけるATP測定を用いた生菌数の迅速定量2014

    • Author(s)
      島﨑大、秋葉道宏
    • Organizer
      第48回日本水環境学会年会
    • Place of Presentation
      東北大学川内キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] 透析用水製造過程におけるATP測定による生菌迅速検査法の開発2013

    • Author(s)
      島﨑大、秋葉道宏
    • Organizer
      第58回日本透析医学会学術集会・総会
    • Place of Presentation
      マリンメッセ福岡
    • Related Report
      2013 Research-status Report
  • [Presentation] 透析用水製造過程におけるATP測定による生菌迅速検査法の開発

    • Author(s)
      島崎大、秋葉道宏
    • Organizer
      第58回日本透析医学会学術集会・総会
    • Place of Presentation
      マリンメッセ福岡
    • Related Report
      2012 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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