Molecular sensing using model membranes

• Project/Area Number: 23560940
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Japan Advanced Institute of Science and Technology
• Principal Investigator: VESTERGAARD Catherine 北陸先端科学技術大学院大学, 大学院教育イニシアティブセンター, 准教授 (20582943)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 免疫センサー / 流動性 / 毒性 / 神経保護 / アミロイド-β / アルツハイマー病 / 電気化学 / 生体模倣センサー / アミロイドβ / 生体模倣膜 / 膜ダイナミクス

Research Abstract

We have (i) shown that amyloid beta (Ab) interacts with membranes in a species-dependent and membrane composition manner. They cause membrane transformation, breach and viscosity. These may contribute towards understanding toxicity mechanisms; (ii) studied how oxidation of cholesterol influences membrane dynamics, demonstrating that temperature increase renders oxidized membranes more fluid. We have similar findings using biological cells. The findings are useful for clarifying the impact of cholesterol in Ab-induced toxicity; (iv) oxidized membranes enhance Ab's association with membranes; (v) potential neuro-protective agents interact with membranes in a structure-dependent manner as does their ability to inhibit Ab aggregation. Unraveling the importance of these compounds, at a functional group level opens the key to tailored design of potential bioactive drug candidates. We have developed an immunosensor chip for Ab.

Research Products

• [Journal Article] Endo- and exocytic budding transformation of slow-diffusing membrane domains induced by Alzheimer's amyloid beta (2014)
  • Author(s): Morita M., Hamada T., Vestergaard M. C., Takagi M.
  • Journal Title: Phys.Chem.Chem.Phys. Volume: 16 Pages: 8773-8773
  • Peer Reviewed
• [Journal Article] Endo- and Exocytic Budding Transformation of Slow-Diffusing Membrane Domains Induced by Alzheimer’s Amyloid Beta (2014)
  • Author(s): M.Morita, T.Hamada, M. C. Vestergaard, M. Takagi
  • Journal Title: Phys. Chem. Chem. Phys. Volume: 16 Issue: 19 Pages: 8773-8777
  • DOI: 10.1039/c4cp00434e
  • NAID: 120005572722
  • Peer Reviewed / Open Access
• [Journal Article] The effect of oxysterols on the interaction of Alzheimer's amyloid beta with model membranes (2013)
  • Author(s): Phan H. T. T., Hata T., Morita M., Yoda T., Hamada T., Vestergaard M. C., Takagi M.
  • Journal Title: Biochim. Biophys. Acta Biomembr. Volume: 1828 Pages: 2487-2487
  • Peer Reviewed
• [Journal Article] Membrane transformation induced by amyloid beta-peptides depend on peptide length, and oligomeric species (2013)
  • Author(s): Vestergaard M. C., Masamune M., Hamada T., Takagi M.
  • Journal Title: Biochim. Biophys. Acta Biomembr. Volume: 1828 Pages: 1314-1314
  • Peer Reviewed
• [Journal Article] Dynamic morphological changes induced by GM1 and protein interactions on the surface of cell-sized liposomes (2013)
  • Author(s): Dhingra S., Morita M., Yoda T., Vestergaard M. C., Hamada T., Takagi M.
  • Journal Title: Materials. Volume: 6 Pages: 2522-2522
  • Peer Reviewed
• [Journal Article] The effect of oxysterols on the interaction of Alzheimer's amyloid beta with model membranes (2013)
  • Author(s): Phan, H.T.T., Hata, T., Morita, M., Yoda, T., Hamada, T., Vestergaard, M.C., Takagi, M.*
  • Journal Title: Biochimica Biophysica Acta, Biomembranes Volume: 1828 Pages: 2487-2495
• [Journal Article] Membrane transformation induced by amyloid beta-peptides depend on peptide length, and oligomeric species, (2013)
  • Author(s): Vestergaard, M.C., Masamune, M., Hamada T., Takagi M*.
  • Journal Title: Biochimica Biophysica Acta, Biomembranes Volume: 1828 Pages: 1314-1321
• [Journal Article] Dynamic morphological changes induced by GM1 and protein interactions on the surface of cell-sized liposomes (2013)
  • Author(s): Dhingra, S., Morita, M., Yoda, T., Vestergaard, M.C., Hamada, T., Takagi, M.*
  • Journal Title: Materials Volume: 6 Pages: 2522-2533
• [Journal Article] Optimization of Functionalized Carbon Nanotube Matrices for Enhanced Ethanol Oxidation Reaction (2013)
  • Author(s): Quynh Hoa, L.Q., Vestergaard, C.M., Yoshikawa, H., Saito, M., Tamiya, E
  • Journal Title: J. Electrochemical Society Volume: 160
• [Journal Article] Membrane fusion and vesicular transformation induced by Alzheimer's amyloid beta (2013)
  • Author(s): Vestergaard, C.M., Morita, M., Hamada, T., Takagi, M.
  • Journal Title: Biophysica et Biochemica Acta (Biomembranes) Volume: 1828
• [Journal Article] Selective localization of Alzheimer's amyloid beta in membrane lateral compartments (2012)
  • Author(s): Morita M., Hamada T., Tendo Y., Hata T. Vestergaard M. C., Takagi M.
  • Journal Title: Soft Matter Volume: 8 Pages: 2816-2816
  • Peer Reviewed
• [Journal Article] Physicochemical profiling of surfactant-induced membrane dynamics in a cell-sized liposome (2012)
  • Author(s): Hamada T., Hagihara H., Morita M., Vestergaard M. C., Tsujino Y., Takagi M.
  • Journal Title: J. Physic. Chem. Lett. Volume: 3 Pages: 430-430
  • Peer Reviewed
• [Journal Article] Size-dependent partitioning of nanoparticles mediated by membrane lateral heterogeneity (2012)
  • Author(s): Hamada T., Morita Miyakawa M., Sugimoto R., Vestergaard M. C., Takagi M.
  • Journal Title: J. Ame. Chem Soc. Volume: 134 Pages: 13990-13990
  • Peer Reviewed
• [Journal Article] Impact of non-covalent interactions within functionalized CNT-grafted catalytic metal electrodes on ethanol oxidation reaction (2012)
  • Author(s): Hoa L. Q., Vestergaard M. C., Yoshikawa H., Saito M., Tamiya E.
  • Journal Title: J. Materials Chem. Volume: 22 Pages: 14705-14705
  • Peer Reviewed
• [Journal Article] Thermo induce dynamics of membranes containing cholesterol derivatives (2012)
  • Author(s): Yoda T., Vestergaard M.C., Hamada T., Le Phuc, T. M., Takagi M.
  • Journal Title: Lipids Volume: 8 Pages: 813-813
  • Peer Reviewed
• [Journal Article] Thermo-induced dynamics of membranes containing cholesterol derivatives (2012)
  • Author(s): Yoda, T., Vestergaard, C.M.*, Hamada, T., Le P.T., Takagi, M.
  • Journal Title: Lipids Volume: 8 Pages: 813-820
• [Journal Article] Size-dependent partitioning of nano/micro-particles mediated by membrane lateral heterogeneity，T (2012)
  • Author(s): Hamada, T., Morita, M., Miyakawa, M., Sugimoto, R., Vestergaard, C.M., Takagi，M.
  • Journal Title: J. Am. Chem. Soc. Volume: 134 Pages: 13990-13996
• [Journal Article] Enhancing catalytic performance of Pt-based electrodes with a noncovalent interaction-induced functionalized carbon nanotube-grafted matrix (2012)
  • Author(s): Quynh Hoa, L.Q., Vestergaard, C.M., Yoshikawa, H., Saito, M., Tamiya, E
  • Journal Title: J. Materials Chemistry Volume: 22 Pages: 14705-14714
• [Journal Article] Selective localization of Alzheimer's amyloid beta in membrane lateral compartments (2012)
  • Author(s): M.Morita、T.Hamada, Y.Tendo, T.Hata, M.C.Vestergaard, M.Takagi
  • Journal Title: Soft Matter Volume: 8 Issue: 10 Pages: 2816-2819
  • DOI: 10.1039/c2sm07185a
  • Peer Reviewed
• [Journal Article] Physicochemical Profiling of Surfactant-Induced Membrane Dynamics in a Cell-Sized Liposome (2012)
  • Author(s): T.Hamada, H.Hagihara, M.Morita, M.C.Vestergaard, Y.Tsujino, M.Takagi
  • Journal Title: The Journal of Physical Chemistry Letters Volume: 3 Issue: 3 Pages: 430-435
  • DOI: 10.1021/jz2016044
  • Peer Reviewed
• [Journal Article] The Effect of oxycholesterols on thermo-sensitivity of lipid vesicles (2011)
  • Author(s): Vestergaard M. C., Yoda T., Hamada T., Ogawa Y., Yoshida Y., Takagi M.
  • Journal Title: Biochim. Biophys. Acta Biomembr. Volume: 1808 Pages: 2245-2245
  • Peer Reviewed
• [Journal Article] A rapid gel electrophoretic chip for serum cholesterol determination (2011)
  • Author(s): Kaminikado K., Ikeda R., Idegami K., Nagatani N., Vestergaard M. C., Saito M., Tamiya E.
  • Journal Title: Analyst Volume: 136 Pages: 1826-1826
  • Peer Reviewed
• [Journal Article] Carbon nanotube structured biomimetic catalyst for polysaccharide degradation (2011)
  • Author(s): Sugano Y., Vestergaard M. C., Saito M., Tamiya E.
  • Journal Title: Chem. Commun. Volume: 47 Pages: 7176-7176
  • Peer Reviewed
• [Journal Article] The Effect of oxycholesterols on thermo-sensitivity of lipid vesicles (2011)
  • Author(s): Vestergaard, CM, Yoda, T., Hamada, T., Ogawa, Y., Yoshida, Y., Takagi, M
  • Journal Title: Biophysica et Biochemica Acta (Biomembranes) Volume: 1808 Issue: 9 Pages: 2245-2251
  • DOI: 10.1016/j.bbamem.2011.05.002
• [Journal Article] A rapid gel electrophoretic chip for serum cholesterol determination. Analyst, 136, 1826 (2011)
  • Author(s): Kaminikado, K., Ikeda, R., Idegami, K., Nagatani, N., Vestergaard, M.C., Saito, M., Tamiya, E
  • Journal Title: Analyst Volume: 136 Issue: 9 Pages: 1826-1830
  • DOI: 10.1039/c0an00992j
• [Journal Article] Functionalized multi-walled carbon nanotubes as supporting matrix for enhanced ethanol oxidation on Pt-based catalysts (2011)
  • Author(s): Hoa,L.Q., Vestergaard, M.C., Yoshikawa, H., Saito, M., Tamiya, E.
  • Journal Title: Electrochemistry Communications Volume: 13 Issue: 7 Pages: 746-750
  • DOI: 10.1016/j.elecom.2011.03.041
• [Journal Article] Dynamic Response of a Cholesterol-containing Model Membrane to Oxidative Stress (2010)
  • Author(s): Yoda T., Vestergaard M. C., Akazawa-Ogawa Y., Yoshida Y., Hamada T., Takagi M.
  • Journal Title: Chem. Lett. Volume: 39 Pages: 1273-1273
  • Peer Reviewed
• [Presentation] A label-free impedimetric Au-NPs immunosensor for amyloid beta peptides (2013)
  • Author(s): Vestergaard M. C., Lien T. T. N., Takamura Y.
  • Organizer: Electrochem 2013
  • Place of Presentation: Southampton, UK
• [Presentation] Lipid bilayer re-organization induced by polyphenols (2013)
  • Author(s): Vestergaard M. C., Chahal B., Phan H. T. T., Yoda T., Takagi M.
  • Organizer: Challenges in Organic Materials and Supramolecular Chemistry (ISACS10)
  • Place of Presentation: Kyoto, Japan
• [Presentation] Amyloid beta mediates membrane fusion (2013)
  • Author(s): Vestergaard M. C., Morita M., Hamada T., Takagi M.
  • Organizer: Alzheimer's Disease and Parkinson's Disease Conference
  • Place of Presentation: Florence, Italy
• [Presentation] A label-free impedimetric Au-NPs immunosensor for amyloid beta peptides. (2013)
  • Author(s): Mun’delanji C. Vestergaard*, Truong T.N. Lien, and Yuzuru Takamura
  • Organizer: Electrochem 2013
  • Place of Presentation: Southampton, UK
• [Presentation] Interaction of Alzheimer’s amyloid beta with oxysterol-containing membranes (2013)
  • Author(s): Huong T.T. Phan, Takahiro Hata, Masamune Morita, Tsuyoshi Yoda, Tsutomu Hamada, Mun'delanji C. Vestergaard, Masahiro Takagi
  • Organizer: International Symposium of the German Society for Biochemstry and Molecular Biology: Molecular Life Sciences
  • Place of Presentation: Frankfurt, Germany
• [Presentation] Amyloid beta mediates membrane fusion. (2013)
  • Author(s): Vestergaard, C.M., Morita, M., Hamada, T., Takagi, M.
  • Organizer: Alzheimer’s and Parkinson’s Disease Congress
  • Place of Presentation: Florence, Italy
• [Presentation] Selective localization of Alzheimer’s amyloid beta in membrane lateral compartments (2013)
  • Author(s): Takagi, M., Morita, M., Vestergaard, C.M., Hamada
  • Organizer: Alzheimer’s and Parkinson’s Disease Congress
  • Place of Presentation: Florence, Italy
• [Presentation] Model Membrane Systems : Introduction and Applications (2012)
  • Author(s): Vestergaard M. C.
  • Place of Presentation: Osaka University, Japan
  • Invited
• [Presentation] Thermo-induced Dynamics of Membranes and Liquid Crystals Containing Cholesterol Derivatives. (2012)
  • Author(s): Yoda, T., Thanh, H.P.T., Vestergaard, C.M., Hamada, T., Takagi, M.
  • Organizer: Micro-NanoMechatronics and Human Science, Nagoya, Japan
  • Place of Presentation: Nagoya, Japan
• [Presentation] Structure-Dependent Membrane Interaction and bioactivity of Flavonoid with Lipid Bilayers. (2012)
  • Author(s): Chahal, B., Vestergaard, C.M., Yoda, T., Morita, M., Takagi, M.
  • Organizer: Micro-NanoMechatronics and Human Science, Nagoya, Japan
  • Place of Presentation: Nagoya, Japan
• [Presentation] The Effect of oxycholesterols on thermo-sensitivity of lipid vesicles (2011)
  • Author(s): Vestergaard M. C., Yoda T., Hamada T., AAkazawa-Ogawa Y., Yoshida Y., Takagi M.
  • Organizer: International Conference, on Micro-NanoMechatronics and Human Science
  • Place of Presentation: Nagoya, Japan
• [Presentation] Amyloid beta interactions with biomimetic membrane systems (2011)
  • Author(s): Vestergaard M. C.
  • Organizer: Nano_Biotechnology field, at Applied Microbiology Symposium, International Union of Microbiological Societies, 2011 Congress
  • Place of Presentation: Sapporo, Japan
• [Presentation] Amyloid beta interactions with biomimetic membrane systems (2011)
  • Author(s): Mun'delanji C. Vestergaard
  • Organizer: International Union of Microbiological Societies Congress(招待講演)
  • Place of Presentation: Sapporo, Japan
• [Book] Lipids, Cholesterol and Oxidized Cholesterol in Alzheimer's Amyloid Beta-mediated Neurotoxicity,"In Metabolic Syndrome and Neurological Disorders" (2013)
  • Author(s): Vestergaard M. C., Morita M., Hamada T., Takagi M.
  • Total Pages: 15
  • Publisher: Wiley-Blackwell Publishers
• [Book] Lipids, Cholesterol and Oxidized Cholesterol in Alzheimer’s Amyloid Beta-mediated Neurotoxicity Chapter in Metabolic Syndrome and Neurological Disorders Editors: Tahira Farooqui and Akhlaq A. Farooqui (2013)
  • Author(s): Vestergaard, C.M., Morita, M., Hamada, T., Takagi, M.
  • Total Pages: 20
  • Publisher: Wiley-Blackwell Publishers
• [Book] In Springer series on Chemical Sensors and Biosensor, "Applications of Nanomaterials in Sensors and Diagnostics" (In Press) (2012)
  • Author(s): Vestergaard, C.M., Saito, M., Yoshikawa, H., Tamiya, E.
  • Publisher: Gold nano-structure LSPR-based biosensor for biomedical diagnosis
• [Book] Gold nano-structure LSPR-based biosensor for biomedical diagnosis, In Springer series on Chemical Sensors and Biosensor, "Applications of Nanomaterials in Sensors and Diagnostics"
  • Author(s): Vestergaard M. C., Saito M., Yoshikawa H., Tamiya E.
  • Publisher: Springer publishers(In Press)
• [Book] Introduction to Nanobiosensors and nanobioanalyses,"In Nanobiosensors and Nanobioanalyses"
  • Author(s): Vestergaard M. C., Tamiya E.
  • Publisher: Springer publishers(In Press)